Rochaniyah, Siti (2018) Potensi Bakteri Serasah Daun Kopi Di Ub Forest Sebagai Pengendali Penyakit Busuk Lunak Pada Umbi Kentang. Sarjana thesis, Universitas Brawijaya.
Abstract
Tanaman kentang (Solanum tuberosum L.) merupakan salah satu jenis tanaman hortikultura dengan nilai ekonomis tinggi, namun produksi masih belum optimal. Salah satu penyebab rendahnya produksi kentang yaitu adanya serangan penyakit busuk lunak disebabkan oleh patogen Erwinia sp. Pengendalian penyakit yang ramah lingkungan dapat menggunakan bakteri antagonis melalui eksplorasi. Sumber eksplorasi bakteri antagonis salah satunya serasah tanaman. UB Forest merupakan hutan pendidikan milik Universitas Brawijaya yang memiliki keanekaragaman hayati yang tinggi dan diperkirakan memiliki jumlah mikroba antagonis yang tinggi juga. Sehingga dilakukan penelitian untuk memperoleh bakteri serasah daun kopi dari kawasan UB Forest yang bersifat antagonis terhadap bakteri patogen Erwinia sp. pada umbi kentang dan mampu mengendalikan penyakit busuk lunak pada kentang. Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya mulai bulan Januari 2017 sampai dengan Juni 2018. Penelitian terdiri dari beberapa tahapan: (1) eksplorasi bakteri serasah daun kopi di kawasan UB Forest (2) uji patogenesitas bakteri Erwinia sp. (3) seleksi bakteri antagonis serasah daun kopi dari kawasan UB Forest terhadap patogen Erwinia sp. (4) uji hipersensitif (5) pengujian antagonis bakteri serasah daun kopi dari kawasan UB Forest terhadap patogen Erwinia sp. (6) uji penghambatan perkembangan penyakit busuk lunak oleh bakteri antagonis serasah daun kopi dari kawasan UB Forest (7) karakterisasi dan identifikasi bakteri serasah daun kopi dari kawasan UB Forest yang bersifat antagonis terhadap patogen Erwinia sp. Hasil seleksi 32 isolat bakteri serasah daun kopi diperoleh 11 isolat bakteri bersifat antagonis terhadap bakteri patogen Erwinia sp. dan empat isolat memiliki indeks penghambatan yang lebih tinggi yaitu isolat 13, 24, 31 dan 32. Dua isolat bakteri serasah daun kopi dari UB Forest yang bersifat antagonis Erwinia sp. memiliki indeks penghambatan lebih tinggi daripada perlakuan streptomisin sulfat 20% secara in vitro yaitu isolat 31 dan 32. Isolat bakteri 31 dan 32 yang menunjukkan kemampuan lebih baik dalam penekanan penyakit busuk lunak pada umbi kentang bila dibandingkan dengan perlakuan kontrol bakterisida streptomisin sulfat 20%. Hasil identifikasi secara morfologi, fisiologi dan biokimia menunjukkan isolat bakteri 13 dan 24 termasuk genus Pantoea sp. sedangkan isolat bakteri 31 dan 32 termasuk genus Erwinia sp.
English Abstract
Potato plant (Solanum tuberosum L.) is one type of horticultural crops with high economic value, but the production is still not optimal. One of the causes of low potato production is the presence of soft rot disease caused by Erwinia sp. The control can use antagonistic bacteria. An exploratory source of antagonistic bacteria is plant litter. UB Forest is an educational forest belonging to Brawijaya University which has high biodiversity and is estimated to have high antagonistic microbial. So the research’s goal to obtain litter bacteria from UB Forest which antagonist to Erwinia sp. on potato tubers and able to control soft rot disease in potatoes. The research was conducted at Plant Diseases Laboratory, Department of Plant Pest and Diseases, Faculty of Agriculture, University of Brawijaya from January 2017 until June 2018. The research consisted of several steps: (1) exploration of litter bacteria in UB Forest (2) pathogenecity test (3) selection of antagonistic of bacteria coffee leaf litter from UB Forest to Erwinia sp. (4) hypersensitive test (5) testing of bacteria antagonist of coffee leaf litter from UB Forest area against Erwinia sp. (6) test of inhibition of the development of soft rot disease by antagonistic bacteria of coffee leaf litter from UB Forest (7) characterization and identification of antagonist bacteria from UB Forest area which is antagonistic to Erwinia sp. Selection of 32 bacterial isolates of coffee leaf litter obtained 11 bacterial isolates were antagonistic to pathogenic bacteria Erwinia sp. and four isolates had a higher inhibition index of 13, 24, 31 and 32 isolates. Two isolates of UB Forest coffee litter bacteria were antagonistic Erwinia sp. has a higher inhibitory index than 20% in vitro streptomycin sulphate treatment are 31 and 32 isolates. Bacteria isolates 31 and 32 show better ability in suppression of soft rot disease in potato tubers when compared with 20% streptomycin sulfate bactericide control treatment. The results of morphological, physiological and biochemical identification showed bacterial isolates 13 and 24 including the genus Pantoea sp. whereas bacterial isolates 31 and 32 belong to the genus Erwinia sp.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/641/051809967 |
| Uncontrolled Keywords: | Bakteri Serasah, Daun Kopi, Penyakit Busuk Lunak, Umbi Kentang |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.9 General topics of pest and disease control |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 31 Oct 2018 02:07 |
| Last Modified: | 19 Oct 2021 14:13 |
| URI: | http://repository.ub.ac.id/id/eprint/13204 |
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