Kajariyah, SitiNur (2015) Eksplorasi Actinomycetes Rhizosfer Dan Endofit Akar Tanaman Cabai (Capsicum Frutescens L.) Serta Uji Antagonismenya Terhadap Jamur Colletotrichum Capsici (Syd.) Bult Et Bisby. Sarjana thesis, Universitas Brawijaya.
Abstract
Actinomycetes dapat digunakan sebagai salah satu agens pengendali hayati untuk pengendalian penyakit tanaman karena kemampuannya dalam menghasilkan antibiotik dan enzim. Actinomycetes banyak ditemukan di tanah yang banyak mengandung bahan organik. Penelitian dilaksanakan di Laboratorium Sentral Ilmu Hayati Universitas Brawijaya Malang yang dilaksanakan pada Januari sampai Mei 2015. Penelitian terdiri dari 3 tahapan yaitu isolasi dan identifikasi C. capsici. Tahap kedua adalah eksplorasi Acinomycetes rhizoser dan endofit akar. Tahap ketiga adalah uji antagonisme isolat Actinomycetes yang ditemukan terhadap patogen C. capsici dan menggunakan Rancangan Acak Lengkap (RAL). Data yang diperoleh diuji dengan menggunakan uji F (5%) dan bila berbeda nyata dilanjutkan dengan uji BNT 5%. Hasil isolasi dan identifikasi didapatkan 3 isolat Actinomycetes yaitu 2 isolat dari rhizosfer dan 1 isolat dari endofit. Hasil penelitian dapat diketahui bahwa isolat Actinomycetes didapatkan termasuk dalam genus Streptomycetes. Berdasarkan hasil analisis isolat Actinomycetes memiliki daya hambat yang berbeda-beda terhadap C. capsici. Isolat Actinomycetes Rhizosfer1 memiliki daya hambat tertinggi yaitu 45,33%, Actinomyecetes Rhizosfer 2 memiliki daya hambat terendah yaitu 41,99% dan Actinomycetes Endofit memiliki daya hambat 44,66%.
English Abstract
Actinomycetes can be used as biological control for controlling plant disease because of it ability to produce antibiotics and enzyme. Actinomycetes found in soil containing large amounts of organic material. The research was conducted at The Center of Biological Sciences Laboratory, Brawijaya University, Malang in January until May 2015. The research comprised in the three stages, first isolation and identification of C. capsici. The second stages is exploration of rhizosphere and root endophytic Actinomycetes. The third stages is antagonism test of Actinomycetes against C. capsici. This test used a Completely Randomized Design. The data obtained were tested using F test 5% and if significantly different, continue by 5% Least Siginificance Different test. Results of isolation and identification obtained 3 isolates namely with details of two isolates of rhizosphere and one isolate of endophytic Actinomycetes. The results of the research showed that Actinomycetes isolates Actinomycetes included in the genus Streptomycetes. Analysis result showed that isolates of Actinomycetes have different inhibit against C. capsici. Isolate Actinomycetes Rhizosphere 1 have the highest inhibitory with 45.33%, Actinomycetes Rhizosphere 2 with 41.99% and Actinomycetes Endophytic is 44.66%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2015/339/ 051504644 |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Kustati |
| Date Deposited: | 09 Jul 2015 09:31 |
| Last Modified: | 09 Jul 2015 09:31 |
| URI: | http://repository.ub.ac.id/id/eprint/130337 |
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