Ardiyati, AlorisaTirta (2015) Uji Patogenisitas Jamur Entomopatogen Beauveria bassiana (Balsamo) Vuillemin pada Jangkrik (Gryllus sp.) (Orthoptera: Gryllidae). Sarjana thesis, Universitas Brawijaya.
Abstract
Jangkrik (Gryllus sp.) merupakan serangga omnivora, serangannya dapat mencapai 83% pada lahan bibit cabai dalam semalam. Pengendalian jangkrik masih relatif sulit. Pada umumnya pengendalian jangkrik dilakukan dengan menyemprotkan insektisida sintetis. Penggunaan insektisida sintetis ini dapat berdampak negatif pada lingkungan maupun organisme lain. Maka perlu adanya alternatif pengendalian yang ramah lingkungan, salah satunya dengan jamur yang bersifat pathogen terhadap serangga yaitu B. bassiana. Penelitian ini bertujuan untuk mengetahui tingkat patogenisitas jamur entomopatogen B. bassiana terhadap Jangkrik (Gryllus sp). Penelitian ini dilaksanakan di Laboratorium Hama Tumbuhan sub Laboratorium Pengembangan Agens Hayati Jurusan Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya Malang. Waktu pelaksanaan penelitian dimulai pada bulan Mei 2014 sampai dengan bulan Agustus 2014. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Metode aplikasi yang digunakan yaitu metode umpan pakan dan metode kontak langsung. Kerapatan konidia B. bassiana yang digunakan yaitu 0 (sebagai kontrol), 104, 106, 108, dan 1010 konidia/ml. Setiap perlakuan menggunakan Gryllus sp. 60 ekor fase nimfa. Pada metode umpan pakan, berupa caisim direndam kedalam suspensi kerapatan konidia B. bassiana kemudian diberikan pada Gryllus sp. sedangkan pada metode kontak langsung dengan cara menyemprotkan langsung suspensi kerapatan konidia B. bassiana ke tubuh Gryllus sp. Pengamatan yang dilakukan meliputi jumlah kematian Gryllus sp. dan gejala yang ditimbulkan Gryllus sp. setelah terinfeksi oleh B. bassiana. Hasil penelitian menunjukkan bahwa pada metode umpan pakan jamur B. bassiana patogenik terhadap Gryllus sp. Konsentrasi efektif (LC50) jamur B. bassiana yang dapat menyebabkan mortalitas 50% Gryllus sp. adalah 7,1 x 106 konidia/ml dengan waktu mematikan (LT50) Gryllus sp. mencapai 50% pada 3,1 hari. Pada metode kontak langsung jamur B. bassiana patogenik terhadap Gryllus sp. Konsentrasi efektif (LC50) jamur B. bassiana yang dapat menyebabkan mortalitas 50% Gryllus sp. adalah 6,2 x 108 konidia/ml dengan waktu mematikan (LT50) Gryllus sp. mencapai 50% pada 4,3 hari.
English Abstract
Crickets (Gryllus sp.) is an omnivora insect, its attack range was 83% on chilli seeds land in one night. Crickets control is still relatively difficult. In general, crickets control is done by spraying synthetic insecticides. The use of synthetic insecticides have a negative impact on the environment and other organisms.therefore, the environmentally friendly alternative control is need it. One of the alternative is using fungi that are pathogenic to insects, namely B. bassiana. The purpose of this research is to determine the pathogenicity degree of B. bassiana entomopathogenic fungi on the crickets (Gryllus sp.). This research was conducted at the Laboratory of Plant Pests sub Laboratory of Biological Agents Development Department of Plant Pests and Diseases Brawijaya University in Malang. The study was begun from May 2014 to August 2014. The research used Complete Randomized Design method with three replications. The used application method was the bait feed method and the direct contact method. The density of used B. Bassiana conidia were 0 (as control), 104, 106, 108, and 1010conidia/ml. Each treatment was using Gryllus sp. 60 nymph phase. In feed bait method, caisin was soaked into the B. Bassiana conidia suspension then it was given to the Gryllus sp. While in the direct contact method, it was by spraying the suspension of B. Bassiana conidia directly to the Gryllus sp. body. Observations were made including the number of Gryllus sp. deaths and symptoms caused by Gryllus sp. after infected by B. bassiana. The results showed that in the bait feeding method, B. Bassiana fungus was pathogenic to Gryllus sp. Effective concentration (LC50) of B. bassiana fungus that can cause mortality of 50% Gryllus sp. was 7,1x106 conidia/ml with lethal time (LT50) Gryllus sp. reached 50% at 3,1 days. In direct contact method, B. Bassiana fungus was pathogenic to Gryllus sp. Effective concentration (LC50) of Bassiana fungus that can cause mortality of 50% Gryllus sp. was 6,2x108 conidia/ml with lethal time (LT50) Gryllus sp. reached 50% at 4,3 days.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2015/149/051502615 |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 06 Apr 2015 10:58 |
| Last Modified: | 19 Oct 2021 06:19 |
| URI: | http://repository.ub.ac.id/id/eprint/130126 |
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I._PENDAHULUAN.pdf Download (1MB) | Preview |
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III._METODOLOGI.pdf Download (1MB) | Preview |
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