Handoko, Andhy (2014) Karakterisasi Penyakit Penting pada Pembibitan Tanaman Durian di Desa Plangkrongan, Kabupaten Magetan dan Pengendalian Dengan Bakteri Antagonis secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Bibit tanaman durian yang dibudidayakan di Desa Plangkrongan beberapa yang terserang penyakit dan mengalami kematian bibit. Jenis patogen yang menyebabkan penyakit pada bibit tanaman durian belum diketahui. Pengendalian penyakit yang ramah lingkungan diperlukan untuk menjaga keseimbangan ekosistem. Salah satu pengendalian yang ramah lingkungan dengan cara menggunakan agen pengendali hayati seperti bakteri antagonis. Penelitian ini bertujuan untuk mengetahui jenis patogen yang menyebabkan kerusakan pada bibit tanaman durian di Desa Plangkrongan, dan mengetahui potensi bakteri antagonis untuk mengendalikan patogen tersebut secara in vitro. Penelitian ini dilakukan dengan beberapa tahap, yaitu tahap pengamatan gejala dan intensitas serangan, tahap isolasi patogen, uji patogenisitas, identifikasi patogen, dan uji antagonis bakteri Bacillus sp. (UB-ABL1), B. subtilis (UB-ABS1), dan Pseudomonas fluorencens (UB-APF1) terhadap pathogen secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), dengan 4 perlakuan, masing-masing perlakuan diulang sebanyak lima kali. Analisis data dilakukan dengan uji F dengan tingkat kesalahan sebesar 5%. Hasil penelitian menunjukkan penyakit penting yang ditemukan pada pembibitan tanaman durian di Plangkrongan adalah hawar daun yang disebabkan oleh patogen yang berupa jamur Fusarium sp. dan bercak daun yang belum diketahui penyebabnya. Bacillus sp. (UB-ABL1), dan B. subtilis (UB-ABS1) efektif menghambat pertumbuhan jamur Fusarium sp. dalam uji antagonis secara in vitro, sehingga berpotensi dikembangkan sebagai agen hayati untuk pengendalian Fusarium sp. penyebab penyakit hawar daun pada bibit tanaman durian.
English Abstract
Some durian seedlings cultured in the Plangkrongan village found infected by plant disease and could cause the seedlings dies. Types of pathogens that cause disease in durian seedlings were still unknown. Environmentally friendly disease control is needed to maintain the balance of the ecosystem. One of the controls that are environmental friendly is by using biological control agents such as antagonistic bacteria. This study aims to determine the type of pathogens that infects durian seedlings in the Plangkrongan village, and to determine the potential of bacterial antagonists for the control of the pathogens in vitro. The study was conducted in several stages, that is observation of symptoms and intensity of the disease, the isolation of the pathogens causing the disease, pathogenicity assay, identification of the pathigens, and in vitro antagonistic assay with antagonistic bacteria Bacillus sp. (UB-ABL1), B. subtilis (UB-ABS1), and Pseudomonas fluorescens (UB-APF1) against the pathogen. Antagonistic experiment used a Complete Randomized Design (CRD) with 4 treatments, each treatment was repeated five times. The data was analyzed with Ftest at with an error rate of 5%. The results showed the important disease found in durian seedling in Plangkrongan is durian leaf blight caused by a fungal pathogen Fusarium sp. and leaf spot disease with unknown causal agent. Bacillus sp. (UB-ABL1), and B. subtilis (UB-ABS1) effectively inhibited the growth of Fusarium sp. in vitro. The result indicated that those antagonistic bacteria were potential to be developed as a biological agent to control the fungus Fusarium sp. the causal agent of leaf blight disease on durian seedlings.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2014/29/051401122 |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 14 Feb 2014 16:06 |
| Last Modified: | 20 Oct 2021 06:51 |
| URI: | http://repository.ub.ac.id/id/eprint/129757 |
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