Dewi, Bella Kusuma (2018) Pengaruh Pemberian Sari Tempe Kedelai Hitam (Glycine Max (L.) Merr.) Hasil Fermentasi Rhizopus Oligosporus Pada Tikus (Rattus Norvegicus) Model Fibrosis Hepar Terhadap Kadar Malondialdehida Dan Aktifitas Alkaline Phosphatase. Sarjana thesis, Universitas Brawijaya.
Abstract
Fibrosis hepar merupakan respon penyakit hepar kronis yang ditandai oleh produksi berlebih matriks ekstraseluler, sehingga berdampak pada perubahan struktur jaringan hepar. CCl4 adalah senyawa hepatotoksik yang dapat menimbulkan stress oksidatif pada hepar sehingga memicu perubahan struktur dari membran sel hepatosit. Kedelai hitam yang terfermentasi oleh Rhizopus oligosporus akan menghasilkan isoflavon lebih tinggi karena terjadi reaksi metabolisme secara anaerob serta menghasilkan enzim fibrinolitik yang dapat menghidrolisis benang fibrin, sehingga berpotensi sebagai terapi alternatif fibrosis hepar. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sari tempe kedelai hitam (Glycine max (L.) Merr.) terhadap kadar MDA dan ALP pada tikus (Rattus novergicus) model fibrosis hepar hasil induksi CCl4. Penelitian ini menggunakan lima kelompok yaitu kelompok kontrol negatif, kelompok kontrol positif yang diinduksi CCl4 20% tiap dua kali perminggu selama dua minggu, kemudian CCl4 25% tiap dua kali sperminggu selama empat minggu sebanyak 0,2 mL/100gBB, kelompok terapi 1,2 dan 3 dengan sari tempe kedelai hitam dosis 200 mg/kgBB, 400 mg/kgBB, 800 mg/kgBB sebanyak 2 mL melalui per oral. Parameter yang diamati adalah kadar MDA dan ALP menggunakan metode spektrofotometer dan dianalisis secara statistik kuantitatif dengan Oneway ANOVA dilanjutkan uji Tukey. Hasil penelitian menunjukkan bahwa terapi sari tempe kedelai hitam secara sangat signifikan (p<0,01) menurunkan kadar MDA dan aktivitas ALP pada tikus model fibrosis hepar. Dosis efektif terapi adalah 800 mg/KgBB dapat menurunkan kadar MDA sebesar 56,3% dan aktivitas ALP sebesar 52,2% dari kontrol positif. Kesimpulan dari penelitian ini yaitu sari tempe kedelai hitam dapat dijadikan terapi untuk menurunkan kadar MDA dan aktivitas ALP pada tikus model fibrosis hepar.
English Abstract
Liver fibrosis is a response to chronic liver disease characterized overproduction of extracellular matrix, so the impact on changes in the structure of the liver tissue. CCl4 is a hepatotoxic model compound that can cause oxidative stress in the liver to trigger a structural change of the hepatocyte cell membrane. Fermented black soybean (Glycine max (L.) Merr.) derived from Rhizopus oligosporus will generate higher isoflavone because anaerobic metabolic reaction and produce fibrinolytic enzyme that can hydrolize fibrine subtrate, so liquid extract of fermented black soybean can be potential therapy of liver fibrosis. This research aimed to determine the effect of fermented black soybean extract on MDA and ALP in rats (Rattus norvegicus) liver fibrosis induced by CCl4. This study was conducted using completely randomized design (CRD), using a rat, which consists of five (5) groups: negative control group, positive control group 20% CCl4 induced twice weekly for 2 weeks, then CCl4 25% induced twice weekly for 4 weeks with volume 0.2 mL/100gBB every two times a week for two weeks later with a volume of 0.1 mL / 100gBW, and therapies group member 1,2 and 3 with given fermented black soybean extract dose 200 mg/kgBW, 400 mg/kgBW, 800 mg/kgBW with volume 2mL per-orally. Measurement of MDA and ALP levels was conducted using spectrophotometer methods and analyzed statistically quantitative with Oneway ANOVA then continued test of Tukey. The results showed that fermented black soybean significantly (p<0,01) decreased MDA levels and ALP activity in mouse hepatic fibrosis model. Efective dose for therapies of 800 mg/KgBW can decrease MDA level for 56,3% and ALP activity for 52,2% from positive control. The conclusion of the study was fermented black soybean extract can be used as therapy to decrease MDA level and ALP activity in mouse model of hepatic fibrosis.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/34/051801432 |
| Uncontrolled Keywords: | Tempe kedelai hitam, fibrosis hepar, MDA, ALP.- Fermented black soybean, liver fibrosis, MDA, ALP. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 74 Drugs derived from spesific plants (Fabales, Legumes, Soybeans) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 02 Nov 2018 07:22 |
| Last Modified: | 21 Oct 2021 08:06 |
| URI: | http://repository.ub.ac.id/id/eprint/12806 |
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