Harsono, Dimas Levin Yustian (2021) Uji Toksisitas Kombinasi Curcumin Dengan Vitamin E Terhadap Ekspresi Inducible Nitric Oxide Synthase (Inos) Dan Gambaran Histopatologi Ginjal Kelinci (Oryctolagus Cuniculus). Sarjana thesis, Universitas Brawijaya.
Abstract
Pengembangan dan pemanfaatan obat herbal di Indonesia perlu mendapatkan substansi ilmiah yang lebih kuat, terutama melalui penelitian dan standarisasi sehingga obat herbal Indonesia dapat diintegrasikan dalam sistem pelayanan kesehatan nasional. Curcumin dan vitamin E merupakan obat herbal yang dipercaya dapat menghambat pertumbuhan sel kanker karena mengandung antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kombinasi curcumin dan vitamin E terhadap ekspresi iNOS dan histopatologi ginjal. Penelitian ini menggunakan 20 ekor kelinci (Oryctolagus cuniculus) jantan dan betina berumur 3 bulan yang dibagi dalam 5 kelompok yaitu kelompok kontrol negatif (P1), kelompok kelinci jantan diberikan kombinasi curcumin dan vitamin E selama 14 hari (P2), kelompok kelinci betina diberikan kombinasi curcumin dan vitamin E selama 14 hari (P3), kelompok kelinci jantan diberikan kombinasi curcumin dan vitamin E selama 28 hari (P4) dan kelompok kelinci betina diberikan kombinasi curcumin dan vitamin E selama 28 hari (P5). Kelinci diberi kombinasi curcumin dosis 72 mg/kgBB dan vitamin E dosis 200 IU secara per oral. Ekspresi iNOS diamati dengan metode imunohistokimia dan pembuatan preparat histopatologi ginjal dengan metode pewarnaan Hematoksilin-Eosin (HE). Hasil penelitian menunjukkan bahwa pemberian kombinasi curcumin dan vitamin E selama 14 hari pada kelinci jantan maupun betina dapat meningkatkan ekspresi iNOS sebesar 0,065% dan 0,11%. Sedangkan pemberian kombinasi curcumin dan vitamin E selama 28 hari pada kelinci jantan maupun betina menunjukkan terjadinya peningkatan ekspresi iNOS sebesar 0,146% dan 0,087%. Gambaran histopatologi menunjukkan tidak terjadi perubahan gambaran histopatologi pada glomerulus dan tubulus organ ginjal kelinci jantan dan kelinci betina. Kesimpulan dari penelitian ini adalah terapi kombinasi curcumin dan vitamin E selama 14 hari dan 28 hari tidak bersifat toksik dan tidak merusak organ ginjal kelinci jantan dan betina.
English Abstract
The development and utilization of herbal medicine in Indonesia needs to get a stronger scientific substance, especially through research and standardization so that Indonesian herbal medicine can be integrated in the national health service system. Curcumin and vitamin E are herbal remedies that are believed to inhibit the growth of cancer cells because they have contain antioxidants. This study aimed to examine the effect of curcumin combination with vitamin E on iNOS expression and renal histopathology. This study used 20 tail males and females of rabbits (Oryctolagus cuniculus) with 3 old month and divided into 5 groups: negative control group (P1), male rabbit group was given combination of curcumin and vitamin E for 14 days (P2), female rabbit group was given combination curcumin and vitamin E for 14 days (P3), male rabbit group was given combination of curcumin and vitamin E for 28 days (P4) and female rabbit group was given combination of curcumin and vitamin E for 28 days (P5). Rabbits were given a combination of curcumin dose of 72 mg/kgBW and vitamin E doses of 200 IU. The therapy conducted per-oral. Expression of iNOS was observed with immunohistochemical methods and the preparation of renal histopathology preparations using the Hematoxylin-Eosin (HE) staining method. The results showed that combination of curcumin and vitamin E for 14 days in both male and female rabbits increased iNOS expression by 0.065% and 0.11%. While combination of curcumin and vitamin E for 28 days in both male and female rabbits showed an increase in iNOS expression of 0.146% and 0.087. The histopathologic view shows no change in histopathologic features of the glomerulus and tubular renal organs of male and female rabbits. The conclusion of this research is combination therapy of curcumin and vitamin E for 14 days and 28 days is not toxic and does not damage the renal organ of male and female rabbits.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/5/051800972 |
| Uncontrolled Keywords: | Curcumin, Vitamin E, Toksisitas, iNOS, Ginjal. Curcumin, Vitamin E, Toxicity, iNOS, Renal. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.324 39 Drugs derived from specific plants (Ginger, Musaceae, Zingiberales) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 01 Nov 2018 02:27 |
| Last Modified: | 24 Feb 2022 04:28 |
| URI: | http://repository.ub.ac.id/id/eprint/12742 |
![[thumbnail of DALAM MASA EMBARGO]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text (DALAM MASA EMBARGO)
Dimas Levin Yustian Harsono.pdf Restricted to Registered users only until 31 December 2023. Download (3MB) |
Actions (login required)
 |
View Item |