Ratnasari, Irmalia (2016) Terapi Pemberian Tepung Daun Mengkudu (Morinda Citrifolia Linn.) Terhadap Kadar Sod (Superoxidase Dismutase) Dan Gambaran Histopatologi Caecum Ayam Broiler Yang Diinfeksi E.Coli. Sarjana thesis, Universitas Brawijaya.
Abstract
Colibacillosis adalah suatu penyakit pencernaan yang disebabkan oleh Avian Pathogenic Escherichia coli (APEC). E. coli menghasilkan endotoksin yang menyebabkan enteritis. Daun mengkudu (Morinda citrifolia L.) mengandung senyawa kimia antraquinone yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui potensi terapi tepung daun mengkudu (Morinda citrifolia linn.) dalam meningkatkan kadar SOD (Superoxide Dismutase) dan memperbaiki gambaran histopatologi caecum ayam broiler yang diinfeksi E.coli. Ayam broiler yang digunakan dalam penelitian ini adalah ayam broiler jantan strain Hubbard umur 21 hari yang dibagi menjadi 5 kelompok perlakuan yaitu kontrol negatif, kontrol positif, dan dosis terapi masing-masing yaitu 3,25 g/ekor/hari, 6,5 g/ekor/hari, 9,75 g/ekor/hari yang diinfeksi bakteri E.coli dosis 0,5 mLx108 CFU/mL/oral. Parameter yang diamati kadar SOD (Superoxide Dismutase) caecum dengan tekhnik spektrofotometri dan histopatologi caecum melalui pewarnaan HE (Hematoksilin Eosin). Hasil penelitian menunjukkan bahwa setelah diterapi tepung daun mengkudu dosis 3,25g/ekor/hari, 6,5 g/ekor/hari dan dosis 9,75 g/ekor/hari secara signifikan (P<0,05) meningkatkan kadar SOD dan memperbaiki gambaran histopatologi caecum ayam broiler yang terinfeksi colibacillosis, ditandai dengan berkurangnya erosi sel epitel silindris, nekrosis sel epitel dan hiperplasia sel goblet. Kesimpulan dari penelitian ini menunjukkan bahwa terapi tepung daun mengkudu (Morinda citrifolia linn.) dengan dosis 9,75 g/ekor/hari merupakan dosis efektif untuk pengobatan colibacillosis pada ayam.
English Abstract
Colibacillosis is a gastrointestinal illness caused by Avian Pathogenic Escherichia coli (APEC). E. coli produced endotoxin which caused enteritis. Noni leaf flour (Morinda citrifolia L.) contain a chemical compound of antraquinon that acts as an antibacterial. This study was aimed to determine the therapeutic effects of noni leaf flour (Morinda citrifolia Linn.) in the increasing level of SOD (Superoxide Dismutase) and repairing caecum histophatological picture of E.coli infected broiler chickens. Broiler chickens used in this study is male Hubbard strain broiler aged of 21 days divided into 5 groups of negative control, positive control, and with theraphy dose 3.25 g/chicken/day, 6.5 g/chicken/day , 9.75 g/ chicken/day. The chicken models were infected by E. coli bacteria 0.5 mLx108 CFU/mL/oral. SOD level caecum were measured using spectrophotometry techniques and the histopathological of caecum observed through HE (Hematoxylin Eosin) staining. The results showed that treatment noni leaf powder dose of 3,25 g/chicken/day, 6,5 g/chicken/day and 9.75 g/chicken/day significantly (P<0.05) increased the levels of SOD and repairing caecum histopathological picture of E.coli infected broiler chicken, measured by erosion cylindrical epithelial cells reduction, necrosis of epithelial cells and hyperplasia of goblet cells. The conclusion of this study indicated that treatment noni leaf flour (Morinda citrifolia Linn.) with theraphy dose of 9.75 g/chicken/day is an effective dose for the treatment of colibacillosis in chicken.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2016/19/ 051602841 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Kustati |
| Date Deposited: | 23 May 2016 10:18 |
| Last Modified: | 23 May 2016 10:18 |
| URI: | http://repository.ub.ac.id/id/eprint/127159 |
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