Rahmawati, RetnoDwi (2016) Pengaruh Terapi Ekstrak Cangkang Siput Air (Pila Ampullacea) Terhadap Gambaran Histopatologi Membran Sinovial Dan Tingkat Apoptosis Kondrosit Melalui Ekspresi Caspase 3 Pada Tikus (Rattus Novergicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Osteoartritis (OA) merupakan suatu gangguan persendian dimana terjadi perubahan berkurangnya tulang rawan sendi dan terjadi hipertropi tulang hingga terbentuk tonjolan tulang pada permukaan sendi (osteopit). Cangkang siput air (Pila ampullacea) memiliki kandungan glukosamin yang dapat digunakan sebagai obat OA. Penelitian ini bertujuan untuk mengetahui pengaruh terapi ekstrak cangkang siput air terhadap gambaran histopatologi membran sinovial dan tingkat apoptosis kondrosit melalui ekspresi caspase 3 pada tikus (Rattus novergicus) osteoartritis hasil induksi papain. Hewan coba dibagi menjadi empat kelompok perlakuan: (1) Kelompok kontrol negatif; (2) Kelompok kontrol positif; (3) Kelompok terapi dengan dosis ekstrak cangkang siput air sebanyak 1 g/kg BB; dan (4) 3 g/kg BB. Pengamatan histopatologi membran sinovial dilakukan dengan pewarnaan HE dan tingkat apoptosis kondrosit dengan teknik imunohistokimia melalui ekspresi caspase 3. Gambaran histopatologi membran sinovial dianalisa secara kualitatif. Sedangkan tingkat apoptosis kondrosit dianalisis secara kuantitatif dengan analisa one way ANOVA (Analysis of Variance) dan dilanjutkan dengan uji Beda Nyata Jujur (BNJ) atau Tukey’s Procedure untuk mengetahui ada tidaknya perbedaan yang nyata (p < 0,05) antar perlakuan menggunakan Statistical Package for the Social Science (SPSS) version 23 for windows. Hasil penelitian menunjukkan terapi ekstrak cangkang siput air secara signifikan (p < 0,05) mampu memperbaiki kerusakan pada membran sinovial yang ditunjukkan dengan penurunan vaskularisasi dan fibrosis, serta mengurangi tingkat apoptosis kondrosit melalui ekspresi caspase 3 dengan dosis terbaik sebesar 3 g/kg BB.
English Abstract
Osteoarthritis (OA) is a joint disorder that cause decrease on joint cartilage and bone hypertropi until formed bone bulge on the joint surface (osteopit). Water snail (Pila ampullacea) shells contains glucosamine which can be used as an OA drug. This research was aimed to determine the effect of water snail shells extract therapy on synovial membrane histopathology and chondrosytes apoptosis level through caspase 3 expression on osteoarthritis rats (Rattus novergicus) induced by papain. Animal model were divided into 4 treatment groups: (1) Negative control group; (2) Positive control group; (3) Therapy groups with dose of water snail shells extract 1 g/kg body weight; and (4) 3 g/kg body weight. Histopathological observation of synovial membrane performed with HE staining and the level of chondrocytes apoptosis by immunohistochemistry technique through caspase 3 expression. Histopathologic features of synovial membrane analyzed qualitatively. While the level of chondrocyte apoptosis were analyzed quantitatively by one way ANOVA (Analysis of Variance), followed by Honestly Significance Difference (HSD) test or Tukey’s Test to determine whether there is a real difference (p < 0,05) between treatments using Statistical Package for the Social Science (SPSS) version 23 for windows. The result showed that therapy of water snail shells extract was significantly (p < 0,05) able to repair damage of synovial membrane that is indicated by decrease in vascularity and fibrosis as well as reduce the level of chondrosytes apoptosis through caspase 3 expression with the best dosage is 3 g/kg body weight.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2016/132/051612867 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Kustati |
| Date Deposited: | 27 Dec 2016 14:40 |
| Last Modified: | 20 Oct 2021 02:32 |
| URI: | http://repository.ub.ac.id/id/eprint/127146 |
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