Prasetya, DarmawanDwi (2015) Suplementasi Chlorogenic Acid (Coffee Robusta Lindau) Pada Tikus (Rattus Norvegicus) Bunting Yang Dipapar Carbon Black Terhadap Penurunan Kadar Estrogen Dan Perbaikan Jaringan Korpus Luteum Ovarium. Sarjana thesis, Universitas Brawijaya.
Abstract
Carbon black merupakan partikulat matter yang bersifat sitotosik, genotoksik dan paling stabil berada di udara dalam bentuk senyawa carbon monoksida (CO). Metabolisme CO dalam tubuh akan mengakibatkan terbentuknya radikal bebas yang mengganggu organ dan produksi hormon. Coffee bean robusta Lindau memiliki senyawa antioksidan dominan dalam bentuk Chlorogenic acid. Tujuan penelitian untuk mengetahui efek suplementasi CGA terhadap kadar estrogen dan perbaikan jaringan korpus luteum ovarium pada tikus bunting yang dipapar carbon black. Penelitian ini merupakan studi eksperimental, post-test control design only menggunakan Rancangan Acak Lengkap. Hewan model yang digunakan adalah tikus (Rattus norvegicus) betina sehat umur 12 minggu dengan berat 100-150 gram. Tikus dibagi menjadi 5 kelompok, yaitu kelompok kontrol negatif, kelompok kontrol positif yang diberikan paparan carbon black 1064 mg/m3 selama 8 jam/hari, kelompok perlakuan yang diberi paparan carbon black 1064 mg/m3 dan suplementasi CGA dengan dosis masing –masing 4,5 ; 9 dan 13,5 mg/kg BB. Kadar estrogen diukur menggunakan ELISA dan dianalisa secara statistik ragam one-way ANOVA dengan uji lanjutan BNJ = 0,05. Pembuatan histopatologi jaringan korpus luteum ovarium menggunakan pewarnaan Hematoksilin Eosin (HE) dan dijelaskan secara deskriptif. Hasil penelitian menunjukkan suplementasi CGA menurunkan kadar estrogen secara signifikan (p<0,05), dengan dosis efektif 4,5 mg/kg BB. Histopatologi korpus luteum menunjukkan berkurangnya nekrosis pada sel luteal korpus luteum. Kesimpulan dari penelitian ini adalah Chlorogenic acid mampu menurunkan kadar estrogen dan mencegah kerusakan jaringan korpus luteum akibat paparan carbon black.
English Abstract
Carbon black is a particulate matter that has properties cytotoxic, genotoxic effects and most stable is in the air as carbon monoxide (CO). Metabolism of CO in the body will lead to the formation of free radical that interfere with organs and hormone production. Green coffee bean Robusta Lindau compound of dominant antioxidant in the form of Chlorogenic acid (CGA). The goal of this research to learn the effects of supplementation CGA toward estrogen level and decreased of ovarian corpus luteum tissue damage in pregnant rats esposured by carbon black. This research was a experimental study post-test control design only and used completed random design. The animal model were healthy female rats (Rattus norvegicus) at age of 12 weeks with 100-150 gram body weight. The rats were divided into five groups, namely Group of negative control, positive control group was given exposure to carbon black of 1064 mg/m3 for 8 hours/day, the treatment group was given the exposure to carbon black of 1064 mg/m3 and supplementation with doses of CGA 4,5 ; 9 and 13,5 mg/kg respectively. Estrogen level were measured by ELISA and statistically analyzed by one-way ANOVA followed by Tuckey test = 0.05. The histopathology of corpus luteum ovary tissue was stained by Hematoxilin Eosin (HE) and analyzed descriptively. The results showed that supplementation of CGA is decrease of estrogen level significantly (p < 0.05), with an effective dose of 4.5 mg/kg BW. Histopathology corpus luteum showed decrease luteal cell necrosis of the corpus luteum. The conclusion of this research is Chlorogenic acid is able to reduce levels of estrogen and prevent corpus luteum tissue damage of pregnant rats that exposured by carbon black.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2015/57/ 051509403 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Kustati |
| Date Deposited: | 22 Feb 2016 15:25 |
| Last Modified: | 22 Feb 2016 15:25 |
| URI: | http://repository.ub.ac.id/id/eprint/127064 |
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