Saputra., Eka Arif Wibawa Hadi (2018) Potensi Bakteri Serasah Tanaman Pinus di UB Forest sebagai Biodegradator Fungisida Mancozeb. Sarjana thesis, Universitas Brawijaya.
Abstract
Salah satu bahan aktif yang banyak terkandung didalam fungisida adalah mancozeb. Fungisida berbahan aktif mancozeb merupakan fungisida yang bekerja secara kontak dan berspektrum luas. Penggunaan mancozeb dikhawatirkan dapat mengganggu kesehatan tanah dan kelangsungan hidup organisme tanah.Salah satu upaya untuk menurunkan atau menghilangkan residu pestisida di lingkungan yaitu dengan melakukan remediasi. Proses remediasi yang menggunakan mikroorganisme dikenal sebagai bioremediasi. Tujuan penelitianuntuk mengetahui manfaat biodiversitas bakteri serasah tanaman pinus di UB Forest dan mengetahui jenis bakteri yang berpotensi sebagai biodegradator fungisida berbahan aktif mancozeb. Penelitian dilaksanakan di UB Forest, kawasan lereng Gunung Arjuno, Dusun Sumbersari, Desa Tawang Argo, Karangploso, Kabupaten Malang dan Laboratorium Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya, Malang mulai bulan Februari 2018 sampai Juni 2018.Penelitian dilakukan dalam5 tahap yaitu: (1) pengambilan sampel serasah(2) isolasi bakteri serasah (3) seleksi bakteri serasah (4) uji biodegradasi fungisida mancozebsecara in vitro (5) Identifikasi bakteri terpilih sampai tingkat genus. Hasil isolasi dari serasah tanaman pinus di UB Forest diperoleh 35 isolat bakteri. Kemudian dilakukan seleksi kemampuan hidup bakteri pada media NA+mancozeb dan uji hipersensitif sehingga diperoleh 9 isolat bakteri. Pada uji biodegradasi fungisida mancozeb secara in vitrohasil rerata diameter Trichoderma sp. setelah hari ke-7 pengamatan menunjukkan bahwa kontrol A (NB) lebih besar dibandingkan dengan perlakuan lain sebesar 8,27 cm dan berbeda nyata dengan kontrol B (NB+mancozeb). Pada perlakuan 9 isolat bakteri diketahui bahwa rerata diameter Trichoderma sp. pada isolat P2, P11, P23, dan P26 tidak berbeda nyata dengan perlakuan kontrol. Sedangkan pada isolat P27, P34, P37, P38, dan P43 menunjukkan hasil yang berbeda nyata dengan perlakuan kontrol. Berdasarkan hasil uji biodegradasi 9 isolat bakteri terdapat 4 isolat bakteri yang menunjukkan hasil rerata pertumbuhan Trichoderma sp. terbesar berturut-turut yaitu P26 sebesar 7,63 cm; P2 sebesar 7,53 cm; P11 sebesar 7,37 cm; dan P23 sebesar 5,27 cm. Hasil identifikasi 9isolat bakteri yang berpotensisebagai biodegardatormerupakan genus Erwinia yaitu isolat P2 dan P34; genus Xanthomonas yaitu isolat P11, P26, dan P27; dan genus Pseudomonas yaitu P23, P37, P38, dan P43.
English Abstract
One of the many active ingredients contained in the fungicide is mancozeb. Fungicide mancozeb is a fungicide that works in contact and broad spectrum. The use of mancozeb is feared to interfere with soil health and the survival of soil organisms. One attempt to reduce or eliminate pesticide residues in the environment is called remediation. The remediation process using microorganisms is known as bioremediation. This study aims to determine the benefits of biodiversity of pine plant litter in UB Forest and to know the type of bacteria potentially as biodegradator fungicide mancozeb. The research was conducted at UB Forest, Arjuno Mountain Slopes, Sumbersari Village, Tawang Argo Village, Karangploso, Malang Regency and Plant Disease Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Brawijaya University, Malang from February 2018 to June 2018. The study was conducted in 5 stages: (1) litter sampling (2) isolation of litter bacteria (3) litter bacterial selection (4) biodegradation test of mancozeb fungicide in vitro (5) Identification of selected bacteria to genus level. The isolation result from pine tree litter in UB Forest obtained 35 bacterial isolates. Then performed the selection of bacterial survival on NA + mancozeb media and hypersensitive test to obtain 9 bacterial isolates. In mancozeb fungicid biodegradation test in vitro the mean diameter of Trichoderma sp. after the 7th day observation showed that control A (NB) was greater than the other treatment of 8.27 cm and was significantly different from control B (NB + mancozeb). In the treatment of 9 bacterial isolates it was known that the average diameter of Trichoderma sp. in isolates P2, P11, P23, and P26 were not significantly different from control treatments. While in isolates P27, P34, P37, P38, and P43 showed significantly different results with control treatment. Based on the result of biodegradation test of 9 bacterial isolates there are 4 bacterial isolates showing the average growth rate of Trichoderma sp. the largest respectively, namely P26 of 7.63 cm; P2 of 7.53 cm; P11 of 7.37 cm; and P23 of 5.27 cm. The identification of 9 potential bacterial isolates as biodegardator were Erwinia genus, namely isolates P2 and P34; genus Xanthomonas ie isolates P11, P26, and P27; and Pseudomonas genus P23, P37, P38, and P43.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/597/051809828 |
| Uncontrolled Keywords: | Bakteri Serasah, Biodegradator, Fungisida Mancozeb, Mikroorganisme |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.9 General topics of pest and disease control > 632.96 Biological control |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 15 Oct 2018 01:59 |
| Last Modified: | 20 Oct 2021 11:30 |
| URI: | http://repository.ub.ac.id/id/eprint/12705 |
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