Ahmada, Rizy (2014) Terapi Ekstrak Daun Putri Malu (Mimosa pudica) Pada Tikus (Rattus norvegicus) Model Asma Terhadap Kadar Malondialdehida (MDA) Dan Gambaran Histopatologi Epitel Bronkiolus. Sarjana thesis, Universitas Brawijaya.
Abstract
Asma merupakan penyakit kronik pada saluran pernafasan yang banyak dijumpai pada hewan dan manusia. Gejala asma yang mucul dapat diperparah oleh infeksi rongga mulut akibat paparan Lipopolisakarida (LPS) dari bakteri Gram negatif. Ekstrak daun Putri malu (Mimosa pudica) digunakan sebagai terapi pada hewan model tikus asma. Tujuan penelitian ini untuk mempelajari potensi terapi ekstrak daun Putri malu (Mimosa pudica) terhadap kadar Malondialdehida (MDA) dan perubahan gambaran sel epitel bronkiolus organ paru pada hewan tikus model asma. Tikus model asma disiapkan dengan pemberian sensitasi alergi dengan Ovalbumin (OVA) secara intraperitonial dan inhalasi serta pemberian LPS dari bakteri Phorphyromonas gingivalis secara intrasulkuler. Penelitian ini menggunakan empat kelompok tikus, yaitu kelompok kontrol negatif, kelompok asma, kelompok terapi daun putri malu dosis 500 mg/Kg BB dan dosis 1000 mg/Kg BB. Kadar MDA diukur menggunakan metode Thiobarbaturic Acid (TBA) dan pengamatan histopatologi epitel bronkiolus menggunakan mikroskop Olympus BX51. Hasil penelitian menunjukkan pemberian terapi daun Putri malu dengan dosis 500 mg/Kg BB dan 1000 mg/Kg BB secara signifikan (p<0,05) mampu menurunkan kadar MDA dan memperbaiki epitel bronkiolus pada tikus model asma. Kesimpulan dari penelitian ini adalah ekstrak daun Putri malu dapat digunakan sebagai terapi herbal pada tikus model asma.
English Abstract
Asthma is a chronic respiratory disease that often found in animals and humans. Asthma symptoms become more severe by oral infections due to exposure of Gram-negative bacterial lipopolysaccharide (LPS). The Mimosa pudica leaf extract were used as an therapy agent on asthma rats. The purpose of this research was to study the potential of Mimosa pudica leaf extract that could affect to Malondialdehyde (MDA) levels and bronchial epithelial histopathology appearance on asthma rats. Asthma rats were prepare by sensitization of allergent conducted by intraperitonial injection and nebulized of Ovalbumin (Ova) also intrasulcular injection of Lipopolysaccharide from Phorphyromonas gingivalis bacteria. Four groups of rats (Rattus norvegicus) were used in this research were control group, asthma group, and two groups with therapy of Mimosa pudica extract dose of 500 mg/Kg BW and 1000 mg/Kg BW. Malondialdehyde levels were determined using Thiobarbaturic Acid (TBA) assay and histophatological observations of the bronchial epithelial using Olympus BX51 microscope. The result showed that Mimosa pudica leaf extract therapy with both dose of 500 mg/Kg BW and 1000 mg/Kg BW significantly (P<0.05) could decrease MDA level and repaired bronchioles epithelial damage in asthma rats. It can be concluded that Mimosa pudica leaf extract have possibility as herbal therapy on asthma rats models.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2014/24/051404732 |
| Uncontrolled Keywords: | Putri malu, Asma, MDA dan Epitel Bronkiolus; Mimosa pudica, Asthma, MDA and Bronchioles epithel |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine > 636.089 5 Veterinary medicine (Pharmacology and therapeutics) > 636.089 532 1 Veterinary medicine (Drugs derived from plants) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Hasbi |
| Date Deposited: | 18 Aug 2014 13:56 |
| Last Modified: | 11 Dec 2021 05:56 |
| URI: | http://repository.ub.ac.id/id/eprint/126910 |
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