Legatawa, Hendra (2013) Peran Yoghurt Terhadap Ekspresi Tumor Necrosis Factor Alpha (TNF-α) dan Gambaran Histopatologi Jaringan Tiroid Tikus Model Autoimmune Thyroiditis (AITD). Sarjana thesis, Universitas Brawijaya.
Abstract
Autoimmune thyroiditis (AITD) adalah penyakit autoimun yang menyerang organ spesifik kelenjar tiroid. Tujuan dari penelitian ini adalah mempelajari peran yoghurt terhadap ekspresi TNF-α dan perbaikan gambaran histopatologi jaringan tiroid tikus (Rattus norvegicus) model AITD. Penelitian dibagi dalam 3 kelompok yaitu kelompok kontrol (A), kelompok AITD yang diinduksi NaI dengan dosis 0,05% selama 5 minggu (B), dan kelompok AITD yang diinduksi NaI dengan dosis 0,05% selama 5 minggu dilanjutkan terapi dengan suplementasi yoghurt sebanyak 109 cfu/ml/hari selama 4 minggu (C). Ekspresi TNF-α diamati dengan menggunakan metode imunohistokimia dan gambaran histopatologi jaringan dengan menggunakan pewarnaan Hematoxilin- Eosin. Terapi yoghurt pada hewan model AITD menimbulkan ekspresi TNF-α yang berbeda nyata pada tiap perlakuan (p<0,05) yaitu 0,446±0,29 % (A); 3,411±1,28 % (B); 1,303±0,53 % (C). Selain itu, yoghurt dapat memperbaiki gambaran histopatologi jaringan tiroid.
English Abstract
Autoimmune thyroiditis (AITD) is a specific-organ diseases appearance in thyroid gland. The aim of this study was to learn the role of yogurt on reducing TNF-α expression and histopatological in thyroid tissue in animal model Autoimmune thyroiditis. In this study, rats were divided into 3 groups : control group, AITD group that induced with NaI 0.05% on five weeks (B), and AITD group that induced with NaI 0.05% in 5 weeks and group that induced with NaI 0.05% in 5 weeks and got the therapy yogurt of 109 cfu/ml/day in 4 weeks (C). In this study, TNF-α expression were observed by immunohistochemistry and the histopathology of thyroid were observed microscopically. Yogurt therapy in animal model of AITD caused different respons of TNF-α expression (p<0.05), were 0.446 ± 0.29% (A), 3.411 ± 1.28% (B), 1.303 ± 0.53 % (C). Besides, yogurt therapy could repair histopathology of thyroid tissue.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2013/23/051308950 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Hasbi |
| Date Deposited: | 16 Oct 2013 09:55 |
| Last Modified: | 19 Oct 2021 04:27 |
| URI: | http://repository.ub.ac.id/id/eprint/126873 |
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