Ramadani, InggilPusvita (2013) Aktivitas Enzim Lipoprotein Lipase (LPL) Serum Dan Gambaran Histopatologi Duodenum Hewan Model Tikus (Rattus norvegicus) Hiperkolesterolemia Yang Mendapat Terapi Ekstrak Air Benalu Mangga (Dendrophto. Sarjana thesis, Universitas Brawijaya.
Abstract
Hiperkolesterolemia merupakan salah satu penyakit gangguan metabolisme kolesterol. Kondisi hiperkolesterolemia memicu terjadinya radikal bebas dan penurunan antioksidan endogen. Tujuan penelitian ini adalah untuk mengetahui pengaruh terapi ekstrak air benalu mangga (Dendrophtoe petandra) terhadap peningkatan aktivitas enzim Lipoprotein Lipase (LPL) dan perbaikan histopatologi duodenum tikus hiperkolesterolemia. Penelitian ini menggunakan empat kelompok tikus (Rattus norvegicus) jantan, yang masing-masing terdiri dari 5 ekor tikus yaitu kelompok kontrol, kelompok hiperkolesterolemia, dan 2 kelompok terapi dosis 400 mg/ekor/hari dan 800 mg/ekor/hari. Diet hiperkolesterol mengandung minyak babi 10%, asam kholat 0,1% dan kuning telur puyuh rebus 5% diberikan melalui metode force feeding. Diet hiperkolesterol diberikan selama 14 hari sebanyak 3,02 gr/2ml/ekor dengan metode sonde. Pembuatan terapi ekstrak air benalu mangga dengan metode infusa dan diberikan selama 14 hari. Penentuan aktivitas enzim LPL dilakukan dengan menggunakan metode titrasi enzimatik sedangkan gambaran histopatologi duodenum diamati secara kualitatif menggunakan mikroskop BX51. Hasil penelitian menunjukkan bahwa terapi ekstrak air benalu mangga (Dendrophtoe petandra) memperbaiki gambaran histopatologi duodenum. Aktivitas enzim LPL secara signifikan (p<0.05) berbeda antar kelompok. Kelompok kontrol memiliki aktivitas paling tinggi yaitu 115,87±3,89 unit. Aktivitas LPL menurun pada kelompok hiperkolesterolemia (33,39±2,06), sebaliknya terapi ekstrak air benalu mangga dengan dosis 400 mg/kg BB dan 800 mg/kg BB aktivitas enzim LPL meningkat berturut-turut 83,69±1,25 unit dan 107,47±1,71 unit. Dapat disimpulkan bahwa terapi ekstrak air benalu mangga (Dendrophtoe petandra) dapat digunakan sebagai alternatif terapi herbal terhadap penyakit hiperkolesterolemia.
English Abstract
Hypercholesterolemia is a disorder of cholesterol metabolism. Hypercholesterolemic conditions trigger free radicals and decrease in endogenous antioxidants. The aim of study was to determine the role of Dendrophtoe petandra extract in hypercholesterolemic rats due to increasing the LPL enzyme activity and improving histopathology condition. The male rats were devide into four groups : control group, hypercholesterolemic group, and 2 groups with therapy of Dendrophtoe petandra extract dose of 400 mg / rat / day and 800 mg / rat / day. Hypercholesterol diet contained 10% lard, 0.1% colic acid and boiled quail egg yolk 5% by force feeding method. Hypercholesterol diet given 14 days as much as 3.03 gram/ 2ml/ rat. Dendrophtoe petandra extract therapy were made by infusa method and given for 14 days. The LPL enzyme activity were measured by the titration method and histopatological of duodenum were determine microscopically by BX51 Olympus microscope. The results showed that the Dendrophtoe petandra extract improved duodenum histopathology. LPL enzyme activity were significantly (P <0.05) different between groups. The control group had the highest activity was 115.87 ± 3.89 units. LPL activity decreased in the hypercholesterolemic group (33.39 ± 2.06), whereas Dendrophtoe petandra extract therapy with a dose of 400 mg / kg and 800 mg / kg BW could increase LPL enzyme activity to be 83.69 ± 1.25 units and 107.47 ± 1.71 units, respectively. It could be conclude that Dendrophtoe petandra extract could use as alternative herbal therapy for hypercolesterolemic.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2013/12/051307311 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Hasbi |
| Date Deposited: | 21 Aug 2013 09:38 |
| Last Modified: | 19 Oct 2021 04:18 |
| URI: | http://repository.ub.ac.id/id/eprint/126862 |
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