Uji Efektifitas Ekstrak Etanol Akar Kemangi (Ocimum Sanctum L.) Terhadap Pertumbuhan Candida Albicans Secara In Vitro.

Sari, AriniNurIndah (2016) Uji Efektifitas Ekstrak Etanol Akar Kemangi (Ocimum Sanctum L.) Terhadap Pertumbuhan Candida Albicans Secara In Vitro. Sarjana thesis, Universitas Brawijaya.

Abstract

Infeksi masih menjadi masalah global, jumlah infeksi yang disebabkan oleh jamur semakin meningkat dan semakin banyak obat yang mengalami resistensi. Salah satu penyakit infeksi jamur adalah kandidiasis yang disebabkan oleh Candida albicans. Berdasarkan hal tersebut diperlukan penelitian untuk alternatif antifungi. Akar kemangi diduga memiliki kandungan senyawa aktif yang dapat digunakan sebagai alternatif antifungi. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol akar kemangi (Ocimum sanctum L.) terhadap pertumbuhan Candida albicans secara in vitro. Sampel Isolat Candida albicans yang diperoleh di Laboratorium Mikrobiologi FKUB. Konsentrasi ekstrak yang digunakan yaitu 8%, 10%, 12%,14%, 16%, 18% dan kontrol jamur. Metode yang digunakan adalah dilusi tabung. Hasil statistik one-way ANOVA menunjukkan perbedaan yang signifikan pada perubahan konsentrasi ekstrak etanol akar kemangi dengan jumlah koloni Candida albicans (p<0,05). Uji korelasi menunjukkan adanya hubungan yang kuat antara ekstrak etanol akar kemangi dengan jumlah koloni Candida albicans (Korelasi r= -9,35; p<0,05). Berdasarkan penelitian ini, dapat disimpulkan bahwa ekstrak etanol akar kemangi mempunyai efek antifungi terhadap pertumbuhan Candida albicans dengan Kadar Bunuh Minimal 18%, sedangkan Kadar Hambat Minimal tidak dapat dievaluasi dengan baik.

English Abstract

Infection is still a global problem, infections caused by fungi is increasing and many are experiencing drug resistance. One disease is fungal infection candidiasis caused by Candida albicans. Based on that research is needed to alternative antifungal. Basil roots suspected to have active compounds that can be used alternative antifungal. This study aims to determine the effectiveness of the ethanol extract of basil roots (Ocimum sanctum L.) on the growth of Candida albicans in vitro. Samples of Candida albicans isolates obtained in the Microbiology Laboratory Medical faculty of Brawijaya. The concentration of the extract used is 8%, 10%, 12%, 14%, 16%, 18% and mildew control. The method used is the dilution tubes. Statistical results of one-way ANOVA showed a significant difference in the change in concentration of the ethanol extract of basil roots the number of colonies of Candida albicans (p <0.05). Correlation test showed a strong relationship between the ethanol extract of basil roots with a number of colonies of Candida albicans (correlation r = -9.35; p <0.05). Based on this study, it can be concluded that the ethanol extract of the basil roots have antifungal effect on the growth of Candida albicans with minimum killing on the concentration 18%, minimum levels can not be evaluated properly.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2016/780/051700488
Subjects: 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.2 Obstetrics
Divisions: Fakultas Kedokteran > Kebidanan
Depositing User: Sugiantoro
Date Deposited: 22 Feb 2017 10:34
Last Modified: 19 Oct 2021 04:02
URI: http://repository.ub.ac.id/id/eprint/126493
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