Wibowo, Adinda Tyas Utami (2018) Uji Efektivitas Media Selektif Untuk Isolasi Trichoderma Spp. Pada Lahan Bawang Prei (Allium Ampeloprasum L.) Organik Dan Konvensional. Sarjana thesis, Universitas Brawijaya.
Abstract
Trichoderma spp. merupakan jenis jamur yang tersebar luas (kosmopolitan) di tanah dan bersifat saprofit. Trichoderma spp. dapat ditemui di hampir semua jenis tanah dan pada berbagai habitat. Isolasi Trichoderma spp. dalam tanah seringkali sulit dilakukan karena pesatnya perkembangan jamur tanah lain pada media agar biasa dapat menghambat pertumbuhan spesies Trichoderma sp. yang berbeda. Sehingga, diperlukan media selektif yang mampu spesifik mengisolasi Trichoderma spp. dari tanah. Pengambilan sampel tanah uji dari UB Forest dan sampel tanah dari lahan bawang prei milik Kelompok Tani Organik di Kelurahan Temas Kota Batu . Pelaksanaan penelitian terdiri dari dua unit percobaan, yang pertama uji daya hambat media terhadap pertumbuhan jamur. Percobaan kedua yaitu Isolasi jamur tanah dari pengolahan lahan organik dan konvensional menggunakan media selektif yang terseleksi. Hasil Uji efektivitas media selektif menunjukkan bahwa media Rose Bengal Chloramphenicol (RBC) yang ditambahkan fungisida berbahan aktif Tembaga oksida 56% mampu menumbuhkan Trichoderma spp dibandingkan media RBC dengan penambahan fungisida berbahan aktif Kaptan, Klorotalonil, dan Karbendazim. Hasil percobaan 1 yaitu isolasi Trichoderma spp. pada lahan UB Forest didapatkan 5 isolat yang dapat tumbuh menggunakan media RBC. Isolat jamur yang didapatkan yaitu Fusarium sp., Trichoderma isolat 1, Trichoderma isolat 2, Trichoderma isolat 3, dan Penicillium sp. Hasil isolasi sampel tanah dari lahan bawang prei didapatkan 4 isolat yang dapat tumbuh. Dari ke empat jenis jamur tersebut, dapat diketahui bahwa pada lahan organik hanya jamur Aspergillus sp. yang tidak tumbuh pada perlakuan konsentrasi 0, sedangkan pertumbuhan jamur lainnya terhambat total pada
English Abstract
Trichoderma is a saprophytic fungi and belongs to the Ascomycota . This fungi also well known as a cosmopolitan genus that can be found in almost all types of soil and habitat. Trichoderma spp usually grow on rhizosphere, the meristematic roots area of plants. Isolation technique of Trichoderma spp from soil is relatively difficult and it has a slow growth type. Therefore, a selective media growth for Trichoderma is needed. Sampling of test soil from UB Forest and soil samples from onion fields owned by Organic Farmers Group in Temas Batu Village. The study consisted of two experimental units, the first was the test of the inhibitory of the media against fungal growth. The second experiment is the isolation of soil fungi from organic and conventional tillage using selective media. The results of the selective media effectiveness test showed that the medium of Rose Bengal Chloramphenicol (RBC) added by fungicide 56% copper oxide capable of growing Trichoderma spp. compared to RBC media with the addition of fungicides Kaptan, Klorotalonil, and Karbendazim. The results of experiment 1 were Trichoderma spp isolation on UB Forest, there were 5 isolates grow using RBC media. Fungal isolates obtained were Fusarium sp., Trichoderma isolate 1, Trichoderma isolate 2, Trichoderma isolate 3, and Penicillium sp. The results of isolation of soil samples from the onion soil obtained 4 isolates grow. From the four types of fungi, it can be seen that on organic land only fungus Aspergillus sp. which was not grow at a concentration of 0, while the growth of other fungi was totally
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/764/051810491 |
| Uncontrolled Keywords: | Trichoderma, Bawang Prei |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.5 Fungi > 579.56 Ascomycotina ( ascomycetes) > 579.567 Pyrenomycetes > 579.567 7 Hypocreales |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 11 Oct 2018 07:53 |
| Last Modified: | 20 Oct 2021 09:06 |
| URI: | http://repository.ub.ac.id/id/eprint/12633 |
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