Artikawati, Desyana (2015) Pengaruh Pemberian Susu Kedelai Terhadap Penurunan Kadar C-Reactive Protein (CRP) Pada Serum Tikus (Rattus norvegicus Galur Wistar) Yang Diberi Diet Tinggi Lemak. Sarjana thesis, Universitas Brawijaya.
Abstract
Diet tinggi lemak menyebabkan peningkatan profil lipid dalam darah terutama Low Density Lipoprotein (LDL). Kolesterol LDL akan teroksidasi mengakibatkan disfungsi endotel sehingga terbentuk plak pembuluh darah. Aterosklerosis merupakan suatu proses inflamasi kronis yang ditandai peningkatan kadar CRP. Pemberian susu kedelai bersifat preventif, mengandung lesitin, sifat melarutkan kolesterol dalam darah, Isoflavon, antioksidan dan mampu meningkatkan High Density Lipoprotein (HDL), serta vitamin E yang mampu mencegah LDL teroksidasi. Penelitian ini adalah penelitian eksperimental laboratorik bertujuan untuk mengetahui pengaruh pemberian susu kedelai terhadap kadar CRP pada serum tikus putih yang diberi diet tinggi lemak. Hewan coba sebanyak 25 ekor tikus jantan, dibagi 5 kelompok yaitu kelompok diet normal (K-), kelompok diet tinggi lemak (K+), dan kelompok perlakuan yang diberi susu kedelai dosis 0,81, 1,62, dan 3,24 gram/ml/tikus/hari. Kadar CRP diukur pada hari ke-91 setelah perlakuan dengan metode imunoturbidimetric. Hasil uji One Way Anova, didapatkan nilai p<α (p=0,011, α=0.05), menunjukkan terdapat perbedaan bermakna antara 5 kelompok perlakuan. Rerata CRP paling tinggi pada kelompok diet tinggi lemak. Hasil uji Post Hoc Tukey tidak didapatkan beda CRP yang bermakna pada ketiga dosis susu kedelai. Kesimpulan penelitian adalah pemberian susu kedelai dapat menurunkan kadar CRP pada serum tikus yang diberi diet tinggi lemak. Disarankan peneliti selanjutnya untuk mengukur marker inflamasi lainnya.
English Abstract
High-fat diet eat to increment of lipid in blood, especially the Low Density Lipoprotein (LDL). The LDL cholesterol will oxidized and lead to endothelial dysfunction, forming a vascular plaque. Atherosclerosis is a chronic inflammatory process that marked increase in CRP levels. The distribution of soya milk was a preventive action. The ingredients of Soya milk include lecithin, has dissolving properties of cholesterol in the blood, isoflavones as an antioxidant and can increase HDL, and also vitamin E which is able to prevent LDL oxidation, antioxidants and can increase high density lipoprotein (HDL), and also contain vitamin E that can prevent oxidized LDL. This study was a laboratory experimental study aimed to determine the effect of soya milk on serum CRP levels in rats fed with a high-fat diet. Experimental animals as much as 25 male rats, divided 5 groups: normal diet (K), high-fat diet group (K +), and the treatment group were given a dose of soya milk 0.81, 1.62, and 3.24 g / ml / rat / day. CRP levels were measured on day 91 after treatment with imunoturbidimetric method. Results of One Way Anova, p-value <α (p = 0.011, α = 0.05), shows that there are significant differences between the 5 treatment groups. The mean CRP was highest in the group of high-fat diet. Post hoc Tukey test results are not obtained CRP were significantly different in all three doses of soya milk. Conclusion of the study is the provision of soya milk can reduce levels of CRP in the serum of mice fed a high-fat diet. It is recommended further research to measure other inflammatory markers.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2015/94/051500926 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel > 610.73 Nursing and services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Ilmu Keperawatan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 09 Feb 2015 22:40 |
| Last Modified: | 09 Feb 2015 22:40 |
| URI: | http://repository.ub.ac.id/id/eprint/125748 |
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