Martapura, Doma (2015) Pengaruh Pemberian Yoghurt Kacang Tunggak (Vigna Unguiculata) Terhadap Kadar Kolesterol LDL Serum Tikus Wistar (Rattus Norvegicus) Yang Diberi Diet Tinggi Lemak. Sarjana thesis, Universitas Brawijaya.
Abstract
Dislipidemia adalah gangguan metabolisme lipoprotein, salah satunya ditandai oleh peningkatan kadar kolesterol LDL. Kacang tunggak merupakan bahan makanan yang mengandung isoflavon yang dapat menghambat enzim HMG-KoA reduktase. Sehingga kacang tunggak mampu menurunkan kadar kolesterol LDL. Tujuan penelitian ini untuk membuktikan pemberian yoghurt kacang tunggak mencegah peningkatan kadar LDL tikus wistar yang diberi diet tinggi lemak. Desain penelitian yang digunakan adalah true eksperimental (Post Test Only Control). Penelitian dilakukan selama 8 minggu dengan menggunakan 30 ekor tikus jantan yang dibagi secara random menjadi 5 kelompok yaitu, kelompok Kontrol Negatif (diet normal), kelompok Kontrol Positif (diet tinggi lemak), kelompok Perlakuan 1 (diet tinggi lemak + yoghurt kacang tunggak dosis isoflavon 1,6 mM/ rata-rata gram BB tikus), kelompok Perlakuan 2 (diet tinggi lemak + yoghurt kacang tunggak dosis isoflavon 2,4 mM/ rata-rata gram BB tikus), dan kelompok Perlakuan 3 (diet tinggi lemak + yoghurt kacang tunggak dosis isoflavon 3,2 mM/ rata-rata gram BB tikus). Variabel yang diukur adalah kadar kolesterol LDL serum dengan menggunakan metode CHOD-PAP dengan sistem photometric. Hasil penelitian menunjukkan bahwa analisa data menggunakan One Way ANOVA yang dilanjutkan dengan uji Post Hoc Tukey HSD diketahui terdapat perbedaan yang signifikan (p=0.000) antar kelompok Kontrol Negatif (diet normal) dan kelompok Kontrol Positif (diet tinggi lemak), terdapat perbedaan yang signifikan antara kelompok Kontrol Positif (diet tinggi lemak) dengan kelompok Perlakuan (diet tinggi lemak + yoghurt kacang tunggak) 1, 2, dan 3. Dapat disimpulkan bahwa pemberian yoghurt kacang tunggak pada kelompok Perlakuan 1,2 dan 3 mampu menurunkan kadar kolesterol LDL pada tikus wistar Rattus Norveigicus yang diberi diet tinggi lemak dengan kadar terendah pada kelompok Perlakuan 3 (diet tinggi lemak + yoghurt kacang tunggak dosis isoflavon 3,2 mM/ rata-rata gram BB tikus).
English Abstract
Dyslipidemia is lipoprotein metabolism disorder which is marked by an increasing of LDL cholesterol level. Cowpea contains a lot of isoflavone that can inhibit the activity of the enzyme HMG-CoA reductase. So, cowpea can be able to decrease LDL cholesterol level. The objective of this study is to see the effects of giving cowpea yogurt on wistar rat’s LDL level which is given high fat diet. Further, this study used true experimental design (Post-test Only Control). The study was conducted for 8 weeks which used 30 male rats and divided randomly into 5 groups i.e. the Negative Control Group (normal diet), the Positive Control Group (high fat diet), the Experiment 1 Group (high fat diet + cowpea yogurt with isoflavone dose 1.6 mM/average of rat’s weight), the Experiment 2 Group (high fat diet + cowpea yogurt with isoflavone dose 2.4 mM/average of rat’s weight), and the Experiment 3 Group (high fat diet + cowpea yogurt with isoflavone dose 3.2 mM/average of rat’s weight). The variable measured was the LDL cholesterol level serum which utilized CHOP-PAP methods with photometric system. Based on the data analysis using One Way ANOVA and Post Hoc Tukey HSD test, it was shown that there were significant differences (p=0.000) between the Negative Control Group (normal diet) and the Positive Control group (high fat diet), and significant differences among the Positive Control Group (high fat diet) and the Experiment Groups (high fat diet + cowpea yogurt) 1, 2, and 3. Thus, it can be concluded that giving cowpea yogurt to the Experiment Groups 1, 2, and 3 was able to decrease LDL cholesterol level on wistar rats (Rattus norveigicus) which were given high fat diet with the lowest level on the Experiment 3 Group (high fat diet + cowpea yogurt with isoflavone dose 3.2 mM/average of rat’s weight).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2015/332/051502620 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel > 610.73 Nursing and services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Ilmu Keperawatan |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 08 Apr 2015 09:09 |
| Last Modified: | 19 Oct 2021 01:04 |
| URI: | http://repository.ub.ac.id/id/eprint/125094 |
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