Aisyah, DewiNur (2015) Efektivitas Ekstrak Etanol Daun Ciplukan (Physalis minima) Sebagai Antifungi dalam Menghambat Pertumbuhan Candida albicans Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Kandidiasis vulvovaginal merupakan salah satu bentuk infeksi pada vagina, yang umumnya menyerang wanita usia subur sedikitnya satu kali selama masa hidupnya dan sekitar 40-50% mengalami kandidiasis vaginalis secara berulang. Infeksi ini sering ditimbulkan oleh jamur C.albicans. obat antifungi yang dipakai saat ini dapat menimbulkan efek samping yang merugikan seperti gangguan alat cerna, iritasi, reaksi alergi, hepatitis, dan gangguan endokrin. Oleh karena itu perlu dilakukan penelitian untuk mendapatkan antifungi alternatif, misalnya dari tanaman ciplukan. Daun ciplukan merupakan tanaman liar yang mengandung alkaloids, flavonoids, saponins, tanin dan terpenoid. Penelitian ini bertujuan untuk membuktikan efek antifungi ekstrak etanol daun ciplukan terhadap pertumbuhan C.albicans secara in vitro. Desain penelitian ini menggunakan eksperimental laboratorium dengan metode dilusi agar untuk menentukan Kadar Hambat Minimum (KHM) dari ekstrak etanol daun ciplukan. Konsentrasi ekstrak etanol daun ciplukan yang digunakan yaitu 5%,6%,7%,8%,9%,10% dan 11%, sedangkan kepadatan C.albicans adalah 104 CFU/ml. Kadar Hambat Minimum (KHM) dari penelitian ini adalah 10%. Hasil uji statistik menunjukkan bahwa terdapat perbedaan efek antifungi pada setiap pemberian konsentrasi ekstrak etanol daun ciplukan terhadap pertumbuhan koloni C.albicans. Hasil Uji Korelasi Spearman adalah R=-0,942 yang berarti semakin tinggi pemberian konsentrasi ekstrak etanol daun ciplukan maka semakin menurunkan pertumbuhan koloni C.albican. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa ekstrak etanol daun ciplukan mempunyai efek antifungi terhadap C.albicans.
English Abstract
Vulvovaginal candidiasis is one of vaginal infection, which affects women in reproductive age at least once during their lifetime, and about 40-50% of them suffered candidiasis vaginalis repeatedly. This infections are often caused by fungus C.albicans. The current antifungal medicine cause adverse side effects such as gastrointestinal disorders, irritation, allergic reactions, hepatitis, and endocrine disorders. Therefore, the study is necessary to acquired an alternative antifungal, example ciplukan plants. Ciplukan leaves are wild plants which containing alkaloids, flavonoids, saponins, tannins and terpenoids. The purpose of this study to prove the antifungal effects of ethanol extract from ciplukan leaves on growth of C.albicans in vitro. This study used an experimental design laboratory with dilution method to determine the minimum inhibitory concentration (MIC) of the ethanol extract from ciplukan leaves. The concentration of ethanol extract from ciplukan leaves that used were 5%, 6%, 7%, 8%, 9%, 10% and 11%, whereas the density of C.albicans was 104 CFU/ml. Minimum inhibitory concentration (MIC) of this study was 10%. The result of statistical test showed that there are differences in antifungal effect at any concentration of ethanol extract from ciplukan leaves on growth of C.albicans colony. The result of Spearman correlation test was R=-0.942, which mean the increase concentration of ethanol extract from ciplukan leaves have inhibited growth of C.albican colony. Based on this study, it can be concluded that the ethanol extract from ciplukan leaves have antifungal effect on C.albicans.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2015/188/051501631 |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.2 Obstetrics |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 26 Feb 2015 07:31 |
| Last Modified: | 22 Oct 2021 07:05 |
| URI: | http://repository.ub.ac.id/id/eprint/124935 |
Preview |
Text
BAB_3.pdf Download (1MB) | Preview |
Preview |
Text
BAB_4.pdf Download (1MB) | Preview |
Preview |
Text
BAB_5.pdf Download (1MB) | Preview |
Preview |
Text
BAB_6.pdf Download (1MB) | Preview |
Preview |
Text
BAB_7.pdf Download (1MB) | Preview |
Preview |
Text
COVER_+_DAFTAR_ISI.pdf Download (1MB) | Preview |
Preview |
Text
DAFTAR_PUSTAKA.pdf Download (1MB) | Preview |
Preview |
Text
LAMPIRAN.pdf Download (2MB) | Preview |
Preview |
Text
ABSTRAK.pdf Download (1MB) | Preview |
Preview |
Text
BAB_1.pdf Download (1MB) | Preview |
Preview |
Text
BAB_2.pdf Download (2MB) | Preview |
Actions (login required)
 |
View Item |