Izza, LinaNurul (2015) Uji Efektifitas Ekstrak Etanol Batang Ciplukan (Physalis minima) Terhadap Pertumbuhan Candida albicans Isolat 1093-SV Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Candida albicans merupakan jamur yang biasa hidup pada populasi manusia tanpa efek berbahaya namun akibat pertumbuhan yang terlalu cepat menyebabkan kandidiasis. Terapi antifungi yang tersedia saat ini memiliki resistensi yang cukup tinggi dan efek samping terapi. Oleh karena itu perlu dilakukan penelitian untuk mendapatkan antifungi alternatif. Salah satunya batang ciplukan yang memiliki kandungan Alkaloid, Flavonoid, Saponin, Tannin, dan Terpenoid. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak etanol batang ciplukan mempunyai efek antifungi terhadap pertumbuhan Candida albicans secara in vitro. Desain penelitian ini menggunakan ekspiremental laboratorium dengan metode dilusi agar yang digunakan untuk menentukan Kadar Hambat Minimum (KHM). Konsentrasi ekstrak etanol batang ciplukan yang digunakan yaitu 15%, 17,5%, 20%, 22,5%, 25% dan 0%, sedangkan konsentrasi Candida albicans adalah 104CFU/ml. Hasil statistik Kruskal wallis p=0,000, kemudian uji dilanjutkan dengan Mann-Whitney dan uji spearman terdapat hubungan antara kenaikan ekstrak etanol batang ciplukan dengan pengurangan jumlah koloni Candida albicans (R=-0,927). Berdasarkan penelitian ini, dapat disimpulkan bahwa ekstrak etanol batang ciplukan mempunyai efek antifungi terhadap Candida albicans dengan Kadar Hambat Minimal (KHM) pada konsentrasi 25%. Perlu dilakukan penelitian lebih lanjut efektifitas ekstrak etanol batang ciplukan dalam menghambat pertumbuhan Candida albicans secara in vivo.
English Abstract
Candida albicans is a fungi that usually grows in the human population without any dangerous effects, however as a consequence to its rapid growth causes candidiasis. The available antifungi therapy has a quite strong resistance and therapy side effects. Thus, a research to find an alternative antifungi needs to be conducted. One of them is stem native gooseberry that contains Alkaloid, Flavonoid, Saponin, Tannin, and Terpenoid. This research aims to prove that the etanol extract of native gooseberry has an antifungi effect towards the growth of Candida albicans in vitro. The research design was laboratorium experimental with the dilution agar method that is used to determine Minimum Inhibitory Concentration (MIC). The stem etanol extract of native gooseberry concetration used are 0%, 15%, 17,5%, 20%, 22,5%, and 25%, while the Candida albicans concetration is 104CFU/ml. The Kruskal wallis statistical result p=0,000, subsequently the test was continued with Mann-Whitney and the spearman test, There is a relation between the increase of stem etanol extract of native gooseberry and the reduction of colony amount of Candida albicans (R=-0,927). Based on this research, it can be concluded that the stem etanol extract of native gooseberry has an antifungi effect towards Candida albicans with a Minimum Inhibitory Concentration (MIC) of 25%. Further research on the effectiveness of stem etanol extract of native gooseberry in obstucting the growth of Candida albicans in vivo manner needs to be done.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2015/152/051501128 |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.2 Obstetrics |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Samsul Arifin |
| Date Deposited: | 17 Feb 2015 11:09 |
| Last Modified: | 22 Oct 2021 06:00 |
| URI: | http://repository.ub.ac.id/id/eprint/124895 |
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