Faridah, Amirah (2015) Efek Antimikroba Ekstrak Etanol Cabai Rawit (Capsicum frutescens L.) terhadap Salmonella Typhi secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Bakteri Salmonella Typhi merupakan patogen penyebab demam tifoid di Indonesia. Ditemukannya MDR (Multi Drug Resistant) Salmonella Typhi mengakibatkan pemilihan alternatif pengobatan menjadi faktor utama yang harus diperhatikan. Alternatif pengobatan yang dikembangkan salah satunya melalui ekstrak tanaman. Salah satu tanaman yang diduga mempunyai efek penghambatan terhadap Salmonella Typhi adalah cabai rawit karena kandungan kapsaisin, alkaloid, flavonoid, dan saponin di dalamnya. Penelitian ini bertujuan untuk mengetahui potensi antimikroba ekstrak etanol cabai rawit (Capsicum frutescens L.) terhadap Salmonella Typhi. Penelitian ini merupakan penelitian eksperimental laboratoris dengan post test only control group design, menggunakan metode dilusi tabung untuk menentukan KHM dan metode penggoresan (streaking) pada medium NAP untuk menentukan KBM. Sampel yang digunakan dalam penelitian ini adalah Salmonella Typhi yang berasal dari Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya, Malang. Konsentrasi ekstrak etanol cabai rawit yang digunakan yaitu 33%, 34%, 35%, 36%, 37%, dan 38% dengan empat kali pengulangan. Hasil penelitian dengan menggunakan metode dilusi tabung menunjukkan KHM pada konsentrasi 34%, dan penggoresan (streaking) pada medium NAP menunjukkan konsentrasi 38% sebagai KBM. Hasil uji statistik One Way ANOVA didapatkan perbedaan signifikan pada perubahan konsentrasi ekstrak terhadap koloni Salmonella Typhi (p < 0,05). Hasil Uji Korelasi-Regresi menunjukkan adanya hubungan yang erat antara konsentrasi ekstrak dengan pertumbuhan bakteri (Korelasi, r = -0,983). Kesimpulan dari penelitian ini adalah bahwa ekstrak etanol cabai rawit (Capsicum frutescens L.) mempunyai pengaruh sebagai antimikroba terhadap Salmonella Typhi secara in vitro dengan kadar hambat minimum (KHM) pada konsentrasi 34% dan kadar bunuh minimum (KBM) pada konsentrasi 38%.
English Abstract
Salmonella Typhi is a pathogenic cause of typhoid fever in Indonesia. The discovery of MDR (Multi Drug Resistant) Salmonella Typhi has made a selection of alternative treatment became a major factor that must be considered. Alternative treatment developed, one of them through the plant extract. One of the plants is considered to have inhibitory effects against Salmonella Typhi is cabai rawit for their content of capsaicin, alkaloids, flavonoids, and saponin. This study aims to determine the antimicrobial potential of the ethanol extract of cabai rawit (Capsicum frutescens L.) against Salmonella Typhi. This study was a laboratory experimental research with post test only control group design, using the tube dilution method to determine the MIC and streaking method on NAP medium to determine MBC. The sample used in this study were derived from Salmonella Typhi Laboratory of Microbiology, Faculty of Medicine, University of Brawijaya, Malang. The concentration of ethanol extract of cabai rawit used were 33%, 34%, 35%, 36%, 37%, and 38% with four repetitions. The results using the tube dilution method showed MIC at a concentration of 34%, and streaking on medium NAP showed a concentration of 38% as MBC. Results of One Way ANOVA statistical test found significant differences in the concentration of the ethanol extract of cabai rawit (Capsicum frutescens L.) on the growth of Salmonella Typhi (p <0.05). The result of the correlation-regression test indicates a close relationship between the concentration of the extract with bacterial growth (correlation, r = -0.983). The conclusion from this study is that the ethanol extract of cabai rawit (Capsicum frutescens L.) has an antimicrobial effect against Salmonella Typhi in vitro with level of minimum inhibitory concentration (MIC) of 34% and minimum bactericidal concentration (MBC) of 38%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/755/051501146 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 13 Feb 2015 14:21 |
| Last Modified: | 22 Oct 2021 06:39 |
| URI: | http://repository.ub.ac.id/id/eprint/124781 |
Preview |
Text
BAB_5.pdf Download (4MB) | Preview |
Preview |
Text
BAB_6.pdf Download (1MB) | Preview |
Preview |
Text
BAB_7.pdf Download (1MB) | Preview |
Preview |
Text
Lampiran.pdf Download (2MB) | Preview |
Preview |
Text
Cover_+_Daftar_Isi.pdf Download (1MB) | Preview |
Preview |
Text
Daftar_Pustaka.pdf Download (1MB) | Preview |
Preview |
Text
Lembar_Pengesahan.pdf Download (1MB) | Preview |
Preview |
Text
Abstrak_Indonesia_+_Inggris.pdf Download (1MB) | Preview |
Preview |
Text
BAB_2.pdf Download (2MB) | Preview |
Preview |
Text
BAB_1.pdf Download (1MB) | Preview |
Preview |
Text
BAB_3.pdf Download (1MB) | Preview |
Preview |
Text
BAB_4.pdf Download (1MB) | Preview |
Actions (login required)
 |
View Item |