Taqwa, Rahmanda (2014) Efek Ekstrak Daun Jambu Biji (Psidii folium) sebagai Penghambat Pembentukan Biofilm Pada Staphylococcus aureus secara in vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Staphylococcus aureus saat ini masih menjadi patogen utama infeksi nosokomial dengan angka kejadian lebih dari 50%. Beberapa strain dari bakteri ini mampu membentuk biofilm pada alat-alat medis seperti kateter urin, sendi prostetik, dan endotracheal tube. Bakteri pembentuk biofilm bersifat resisten terhadap tekanan lingkungan dan terapi antibiotika. Oleh karena itu, perlu dilakukan penelitian untuk mencari senyawa yang dapat menghambat pembentukan biofilm. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daun jambu biji yang mengandung flavonoid, saponin, minyakatsiri, tannin dalam menghambat pembentukan biofilm S. aureus. Desain penelitian ini adalah true experimentalpost-test only group design. Konsentrasi ekstrak daun jambu biji yang digunakan adalah 0% (kontrol), 0.125%, 0.25%, 0.5%, 1%, dan 1.5% kemudian dilakukan pengulangan 4 kali. Pembentukan biofilm diamati dengan metode tabung kemudian diukur mean gray valuenya dengan program Adobe Photoshop CS3. Hasil statistik Uji One Way ANOVA menunjukkan adanya perbedaan yang signifikan pada perubahan konsentrasi ekstrak daun jambu biji terhadap mean gray value (p < 0,05). Uji Korelasi Pearson menunjukkan adanya hubungan yang sangat kuat antara konsentrasi ekstrak dengan mean gray value (r = 0,885). Dari hasil penelitian ini disimpulkan bahwa ekstrak daun jambu biji dapat menghambat pembentukan biofilm dengan Minimum Biofilm Inhibitory Concentration (MBIC) sebesar 1.5%.
English Abstract
Nowadays Staphylococcus aureus still become the main pathogen of nosocomial infection with prevalence more than 50%.Some of these strains could form biofilm in medical devices such as urinary catheter, prosthetic joint, and endotracheal tube.With biofilm, bactery has a resistant charactheristics towards the environment and antibiotics therapy. Therefore, it is necessary to find a compound that can inhibit biofilm formation. This study was conducted to prove the effect of guava leaves extract that contain flavonoid, saponin, volatile oil, tannin as S. aureus biofilm formation inhibitor. This research is using post-test only group design. The concentrations of guava leaves extract used were 0% (control), 0.125%, 0.25%, 0.5%, 1%, and 1.5% then we repeated the experiment until 4 times. The biofilm formation was observed with tube method, mean gray value was measured with Adobe Photoshop CS3 program. The result of One Way ANOVA showed that there was a significant difference in various extract’s concentration given to S. aureus (p < 0,05). The result of Pearson test showed that there was a very strong association between concentration of the extract and mean gray value (r = 0,885). The conclusion is the guava leaves extract could inhibit S. aureus biofilm formation with the Minimum Biofilm Inhibitory Concentration (MBIC) at 1.5%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/615/051406750 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 21 Oct 2014 10:39 |
| Last Modified: | 22 Oct 2021 03:33 |
| URI: | http://repository.ub.ac.id/id/eprint/124628 |
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