Auli, MeutiaTamimi (2014) Efek Pemberian Ekstrak Biji Jintan Hitam (Nigella sativa) Terhadap Konsentrasi GLUT-4 pada Tikus Putih (Rattus norvegicus) Strain Wistar Model Diabetes Melitus Tipe 2. Sarjana thesis, Universitas Brawijaya.
Abstract
Diabetes Melitus (DM) merupakan masalah yang banyak terjadi di negara berkembang, salah satunya adalah di Indonesia.DM tipe 2 ditandai dengan keadaan resistensi insulin yang dapatterjadi karena penurunan sintesisdan translokasi GLUT-4 ke permukaan sel. Penelitian ini bertujuan untuk mengetahui efek ekstrak biji jintan hitam terhadap konsentrasi GLUT-4 di jaringanotot . Desainpenelitianiniadalah true experimental dengansampel 25 ekortikusputihjantan strain Wistar model DM tipe 2 yang dibagikedalam 5 kelompokyaitu kontrol negatif ( 10% tween 80 ), kontrol positif (metformin 75 mg/kgBB ), 3 kelompok mendapat ekstrak biji jintan hitam dengan dosis yang berbeda (24 mg/kgBB, 48 mg/kgBB, dan 96 mg/kgBB). Induksi DM tipe 2 dilakukan selama 2 bulandenganmenggunakanpakantinggikalori yang mengandung 10% lemakbabidan 20% sukrosasertainjeksi STZ 30 mg/kgBB, laludilakukanterapiselama 1 bulan. Konsentrasi GLUT-4 diukurdenganmenggunakan ELISA, hasilnyatidakterdapatperbedaankonsentrasi GLUT-4 yang signifikanantarkelompok (ANOVA, p > 0,05).
English Abstract
Diabetes Mellitus (DM) is a common problem in developing countries, including in Indonesia. Type 2 diabetes is characterized by insulin resistance that occur sbecause of GLUT-4 synthesis and translocation to cell surface reduction . The aim of this study was to determine the effects of black seed extract to the concentration of GLUT-4 in muscle tissue. T rue experimental design was used in this study , 25 male Wistar rats model type 2 DM were divided into 5 groups: negative control (10% tween 80), positive control (metformin 75 mg/kg), 3 groups received black seed extract in different do sages (24 mg/kg, 48 mg/kg, and 96 mg/kg). Induction of type 2 diabetes was conducted for 2 months using high energy diet containing 10% lard and 20% sucrose combined with STZ injection 30 mg/kg, followed by 1 month therapy . GLUT-4 concentration was measured by ELISA method , the result of this study indicate that there was nostatistical ly different concentration of GLUT-4 between each group (A NOVA , p >0,05) .
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/499/051403768 |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) |
| Divisions: | Fakultas Kedokteran > Farmasi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 14 Jul 2014 09:40 |
| Last Modified: | 22 Oct 2021 01:44 |
| URI: | http://repository.ub.ac.id/id/eprint/124499 |
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