Ichtivani, Mia (2014) Efek Ekstrak Biji Pare (Momordica charantia) dalam Menghambat Peningkatan Kadar IL-6 Serum pada Tikus (Rattus norvegicus) Wistar dengan Diet Aterogenik. Sarjana thesis, Universitas Brawijaya.
Abstract
Aterosklerosis adalah penebalan dinding arteri karena respon inflamasi kronis terhadap cedera endotel yang melibatkan faktor peradangan IL-6. Biji pare (Momordica charantia) mengandung saponin yang terbukti mampu menurunkan kadar IL-6 pada proses aterosklerosis. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak biji pare yang mengandung saponin dapat menghambat peningkatan kadar IL-6 serum. Studi true experimental dengan metode randomized posttest only controlled group design. Total 25 ekor tikus Wistar jantan dibagi menjadi 5 kelompok: kelompok diet aterogenik sebagai kontrol(+), kelompok diet aterogenik dengan ekstrak biji pare dosis 150 μg/gBB, kelompok diet aterogenik dengan esktrak biji pare dosis 300 μg/gBB, kelompok diet aterogenik dengan ekstrak biji pare dosis 500 μg/gBB, serta kelompok diet normal sebagai kontrol(-), masing-masing selama 12 minggu. Ekstrak biji pare diberikan mulai minggu 8. Setelah 12 minggu dilakukan ELISA dengan sampel serum. Selanjutnya dilakukan pengukuran kadar IL-6 serum dengan metode ELISA. Uji One-way ANOVA menunjukkan ada perbedaan bermakna kadar IL-6 serum antar kelompok (p=0,000), sementara uji Tukey menunjukkan bahwa kadar IL-6 kelompok dosis 300 μg/gBB dan kelompok dosis 500 μg/gBB lebih rendah signifikan dibanding kelompok diet aterogenik (p<0,05), dan tidak memiliki perbedaan dibanding kontrol(-) (p>0,05). Hal ini menunjukkan bahwa dosis 300 dan 500 μg/gBB potensial dalam menghambat peningkatan kadar IL-6 serum. Uji korelasi antar kelompok perlakuan diperoleh nilai R=-0,753 (p=0,000). Kesimpulan penelitian ini adalah pemberian ekstrak biji pare (Momordica charantia) terbukti dapat menghambat peningkatan kadar IL-6 serum tikus Wistar dengan diet aterogenik.
English Abstract
Atherosclerosis is artery wall thickening due to chronic inflammation to endothelial injury which involves inflammatory factor IL-6. Meanwhile, bitter melon (Momordica charantia) seed contains saponin, which had been proven reducing IL-6 in atherosclerosis process. This study is aimed to prove that bitter melon seed extract which is thought to contain saponin can inhibit serum IL-6 level. This true experimental study uses randomized posttest only controlled group design. Total 25 rats were devided in 5 groups: atherogenic diet group as control (+), atherogenic diet group with bitter melon seed extract dose of 150 μg/gBB, atherogenic diet group with the seeds of bitter melon extracts dose of μg/gBB, ath-erogenic diet group with bitter melon seed extract dose of 500 μg/gBB, as well as group normal diet as control (-), respectively for 12 weeks. Bitter melon seed extract given from week 8. After 12 weeks, ELISA performed with serum sample. Next step, serum IL-6 level was measured by ELISA method. One-way ANOVA test showed that there was significant difference serum IL-6 level between all groups (p=0,000), while Tukey test showed that IL-6 level in 300 μg/gBB doses group and 500 μg/gBB doses group significantly lower than the atherogenic diet group (p <0.05), and no differences compared to normal diet group (p> 0.05). This suggests that the dose of 300 and 500 μg/gBB potent in inhibiting the elevated levels of serum IL-6. Correlation test between treatment groups showed the value of R=-0,753 (p=0,000). Conclusion of this study is bitter melon (Momordica charantia) seed extract administration was proven inhibiting serum IL-6 level in Wistar rat with atherogenic diet.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/450/051403572 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 30 Jun 2014 10:44 |
| Last Modified: | 21 Oct 2021 17:39 |
| URI: | http://repository.ub.ac.id/id/eprint/124446 |
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