DerisnaCS, AnakAgung (2014) Pengaruh Vaksin Heat Killed Salmonella Typhimurium Terhadap Kadar Tumor Necrosis Factor Alpha (TNF-α) Serum pada Tikus Wistar Jantan yang Diberi Diet Tinggi Lemak. Sarjana thesis, Universitas Brawijaya.
Abstract
Salmonella Typhimurium yang menyintesa phosphorylcholine (PC) mampu menginduksi respon antibodi anti-PC yang bereaksi silang dengan PC pada oxidized lipoprotein (Ox-LDL). Tujuan dari penelitian ini untuk mengetahui efek pemberian vaksin heat killed bakteri Salmonella Typhimurium dalam menurunkan kadar TNF-α serum pada tikus yang diberi diet tinggi lemak. Penelitian ini merupakan penelitian eksperimental laboratorik. Vaksin dibuat dengan cara memanaskan 108 bakteri S. Typhimurium pada suhu 1000 C selama 45 menit di atas waterbath. Dua puluh lima ekor tikus jantan galur Wistar sebagai hewan coba, dibagi ke dalam satu kelompok kontrol negatif (diet normal) dan empat kelompok perlakuan diet tinggi lemak terdiri dari kelompok kontrol positif (tanpa vaksinasi), kelompok A (vaksin 100 μl ditambah ajuvan CFA-IFA 100 μL), kelompok B (vaksin 100 μL), dan kelompok C (ajuvan CFA-IFA 100 μL). Vaksin diinjeksikan dua minggu sekali sebanyak lima kali, dan terdiri atas injeksi primer dan empat injeksi booster. Hasil penelitian menunjukkan bahwa pemberian vaksin heat killed S. Typhimurium ditambah CFA-IFA dapat menurunkan jumlah kadar TNF-α serum secara signifikan dibanding kelompok kontrol positif (p < 0,05). Kesimpulan penelitian adalah pemberian vaksin heat killed Salmonella Typhimurium dengan ajuvan CFA-IFA terbukti menurunkan kadar TNF-α serum sebagai sitokin proinflamasi. TNF-α adalah sitokin proinflamasi yang mempunyai peranan dalam progesivitas sindroma metabolik. Perlu dilakukan penelitian lebih lanjut mengenai potensi heat killed S. Typhimurium untuk mencegah progesivitas sindroma metabolik.
English Abstract
Salmonella Typhimurium that synthesize phosphorylcholine (PC) was able to induce anti-PC antibody responses that cross-react with the PC on oxidized lipoprotein (Ox-LDL) .The purpose of this study was to determine the effect of heat killed bacteria vaccine Salmonella Typhimurium in lowering levels of serum TNF-α in rat fed by high fat diet. This study was an experimental research laboratory. The vaccine was made by heating 108 S. Typhimurium bacteria at temperature of 100°C for 45 minutes on the waterbath. Twenty five male Wistar rats as experimental animals, divided into the negative control group (normal diet) and four treatment groups high fat diet consisting of the positive control group (without vaccination), group A (vaccine 100 μl plus CFA-IFA adjuvant 100 μL), group B (vaccine 100 μL), and group C (CFA-IFA adjuvant 100 μL). The vaccine was injected five times every two weeks, where the vaccine consist of primary injection and four booster injection. The results showed that administration of heat killed S. Typhimurium vaccine plus CFA-IFA can lower the amount of serum levels of TNF-α significantly to the positive control group (p < 0,05). The conclusion is the research proved that heat killed Salmonella Typhimurium vaccine with CFA-IFA adjuvant decreases TNF-α serum which are proinflammantory cytokine. TNF-α is proinflammantory cytokine which has a role in the progression of metabolic syndrome. Further researches about the potential of heat killed S. Typhimurium towards the prevention of the progression of metabolc syndrome are needed to be conducted.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/398/051403431 |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 25 Jun 2014 10:10 |
| Last Modified: | 21 Oct 2021 14:50 |
| URI: | http://repository.ub.ac.id/id/eprint/124387 |
Preview |
Text
BAB_4.pdf Download (2MB) | Preview |
Preview |
Text
BAB_5.pdf Download (1MB) | Preview |
Preview |
Text
BAB_6.pdf Download (1MB) | Preview |
Preview |
Text
BAB_7.pdf Download (1MB) | Preview |
Preview |
Text
DAFTAR_PUSTAKA.pdf Download (1MB) | Preview |
Preview |
Text
COVER_DAN_DAFTAR_ISI.pdf Download (1MB) | Preview |
Preview |
Text
LAMPIRAN.pdf Download (3MB) | Preview |
Preview |
Text
ABSTRAK_GABUNGAN_(INDONESIA_DAN_INGGRIS).pdf Download (1MB) | Preview |
Preview |
Text
BAB_1.pdf Download (1MB) | Preview |
Preview |
Text
BAB_2_.pdf Download (2MB) | Preview |
Preview |
Text
BAB_3.pdf Download (1MB) | Preview |
Actions (login required)
 |
View Item |