Kusumaningrum, Ratih (2014) Uji Efek Antibiotik Ekstrak Etanol Tanaman Putri Malu (Mimosa pudica) Terhadap Bakteri Salmonella Typhi Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Salmonella Typhi merupakan penyebab demam tifoid yang memiliki resistensi terhadap antibiotik. Oleh karena itu diperlukan obat alternatif. Tanaman putri malu memiliki kandungan alkaloid, flavonoid, dan tanin. Penelitian ini bertujuan untuk mengetahui ekstrak tanamanan putri malu sebagai antibiotik terhadap S. Typhi secara in vitro. Desain penelitian yang digunakan adalah penelitian eksperimental laboratorik dengan post test control group design only. Metode yang digunakan adalah difusi cakram yang digunakan untuk menentukan Kadar Hambat Minimum (KHM) dan metode dilusi tabung untuk menentukan Kadar Bunuh Minimum (KBM). Ekstrak diperoleh melalui metode maserasi dengan pelarut etanol 96% Konsentrasi ekstrak yang dipakai yaitu 5%, 7%, 9%, 11% dan 13%. Hasil statistik Kruskal Wallis menunjukkan terdapat perbedaan pada perubahan konsentrasi ekstrak etanol tanaman putri malu terhadap jumlah koloni S. Typhi (p<0,05). Uji korelasi menunjukkan adanya hubungan antara konsentrasi ekstrak dengan jumlah koloni, dimana semakin meningkat konsentrasi ekstrak etanol tanaman putri malu pertumbuhan S.Typhi semakin menurun. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol tanaman putri malu mempunyai efek antibiotik terhadap S. Typhi secara in vitro dengan Kadar Hambat Minimum (KHM) 5% dan Kadar Bunuh Minimum (KBM) adalah 13%.
English Abstract
Salmonella Typhi is known as causes of typhoid fever that possess resistance against antibiotics. Therefore we need an alternative medicine. Sensitive plant which contains alkaloids, flavonoids and tannins. This study aims to determine sensitive plant extractas an antibiotical against S. Typhi in vitro. Using experimental laboratorium with post test control group design only. The method used is the disk diffusion consisting of phase determination Minimum Inhibitory Concentration (MIC) and tube dilution method to determine Minimum Bactericidal Concentration (MBC). Sensitive plant extract is made by Maceration extraction using ethanol 96%. The concentration used were 5%, 7%, 9%, 11%, and 13%. By Kruskal Willis method shows differences in concentrations changes of extract of sensitive plant to the number of S. Typhi colonies (p<0.05). Correlation test shows the relationship between the concentration of the extract with the number of colonies, which is the higher concentrations of ethanol extract of sensitive plant reduce the growth of S. Typhi. Based onthe result, it can be concluded that the ethanol extract of sensitive plant has an antibiotical effect against S. Typhi in vitro with the Minimum Inhibitory Concentration (MIC) is 5% and Minimum Bactricidal Concentration (MBC) is 13%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/392/051403393 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 24 Jun 2014 08:41 |
| Last Modified: | 21 Oct 2021 14:03 |
| URI: | http://repository.ub.ac.id/id/eprint/124381 |
Preview |
Text
COVER.pdf Download (1MB) | Preview |
Preview |
Text
DAFTAR_PUSTAKA.pdf Download (1MB) | Preview |
Preview |
Text
LAMPIRAN_2.pdf Download (1MB) | Preview |
Preview |
Text
LAMPIRAN_3.pdf Download (1MB) | Preview |
Preview |
Text
ABSTRAK.pdf Download (1MB) | Preview |
Preview |
Text
LAMPIRAN_1.pdf Download (1MB) | Preview |
Preview |
Text
BAB_1.pdf Download (1MB) | Preview |
Preview |
Text
BAB_2.pdf Download (2MB) | Preview |
Preview |
Text
BAB_3.pdf Download (1MB) | Preview |
Preview |
Text
BAB_4.pdf Download (2MB) | Preview |
Preview |
Text
BAB_5.pdf Download (2MB) | Preview |
Preview |
Text
BAB_6.pdf Download (1MB) | Preview |
Preview |
Text
BAB_7.pdf Download (1MB) | Preview |
Actions (login required)
 |
View Item |