Roragabar, NoraAsri (2014) Uji Efektivitas Ekstrak Daun Kenanga (Cananga odorata) sebagai Antimikroba Terhadap Pertumbuhan Vibrio cholerae in Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Vibrio cholerae adalah salah satu patogen penyebab diare pada anak maupun dewasa. Vibrio cholerae cepat menjadi resisten terhadap banyak obat antimikroba sehingga menimbulkan masalah terapi yang sulit. Salah satu alternatif terapi adalah dengan bahan alami, yaitu ekstrak daun kenanga (Cananga odorata). Kandungan aktif daun kenanga yang diduga bermanfaat sebagai antimikroba adalah saponin, flavonoid and polifenol. Penelitian ini bertujuan untuk mengetahui potensi antimikroba ekstrak daun kenanga (Cananga odorata) terhadap Vibrio cholerae. Penelitian ini merupakan penelitian eksperimental laboratoris murni dengan post test only control group design, menggunakan metode dilusi tabung. Sampel yang digunakan dalam penelitian ini adalah Vibrio cholerae yang diperoleh dari Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya, Malang. Konsentrasi ekstrak daun kenanga yang digunakan yaitu 20%, 21%, 22%, 23%, dan 24% dengan lima kali pengulangan. Hasil uji statistik One Way Anova menunjukkan terdapat perbedaan yang signifikan pada perubahan konsentrasi esktrak daun kenanga (Cananga odorata) terhadap pertumbuhan Vibrio cholerae (p<0,05). Post Hoc test (Tukey’s Test) menunjukkan bahwa terdapat penurunan jumlah koloni Vibrio cholerae yang signifikan seiring dengan peningkatan dosis ekstrak daun kenanga (Cananga odorata). Uji korelasi Spearman menunjukkan adanya hubungan yang erat antara konsentrasi ekstrak dengan pertumbuhan bakteri (Korelasi, r = -0,983; p<0,05). Kesimpulan penelitian ini yaitu ekstrak daun kenanga (Cananga odorata) mempunyai pengaruh sebagai antimikroba terhadap Vibrio cholerae dengan kadar hambat minimum (KHM) 21% dan kadar bunuh minimum (KBM) 24%.
English Abstract
Vibrio cholerae is one of pathogens caused diarrhoea in children and adults. Vibrio cholerae is quickly becoming resistant to many antimicrobial drugs and lead to a difficult theraupetic problems. One natural alternative therapy that can be used is cananga (Cananga odorata) leaf extract. The active compositions of cananga leaf (Cananga odorata) which allegedly useful as antimicrobial are saponin, flavonoid and polyphenol. This study aims to determine the antimicrobial potency of cananga (Cananga odorata) leaf against Vibrio cholerae. This study is an experimental research laboratory using post test only control group design, done by tube dilution method. Samples used in this study were Vibrio cholerae derived from Microbiology Laboratory of Medical Faculty Brawijaya University, Malang. Consentration of the cananga leaf extract used is 20%, 21%, 22%, 23%, and 24% with five repetitions. One Way Anova test results showed statistically significant difference in changes in the concentration of cananga (Cananga odorata) leaf in extracts on the growth of Vibrio cholerae (p<0.05). Post Hoc test (Tukey’s Test) showed that there was significant decreasing amount of Vibrio cholerae colonies together with the increasing dose of extract cananga (Cananga odorata). Spearman correlation test showed a close relationship between the concentration of the extract with bacterial growth (correlation, r = -0.983, p<0.05). Conclusion of the research is extract of cananga (Cananga odorata) leaf has effect as an antimicrobial against Vibrio cholerae which the level of Minimun Inhibitory Concentration (MIC) is 21% and Minimum Bactericidal Concentration (MBC) is 24%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/391/051403365 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 20 Jun 2014 09:55 |
| Last Modified: | 21 Oct 2021 13:53 |
| URI: | http://repository.ub.ac.id/id/eprint/124380 |
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