Pradana, EndyWira (2014) Uji Efektivitas Ekstrak Etanol Daun Ceplukan (Physalis minima L.) Terhadap Streptococcus mutans secara in vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Streptococcus mutans merupakan bakteri Gram positif dalam rongga mulut yang metabolismenya menghasilkan zat asam yang menjadi penyebab umum karies (gigi berlubang). Untuk menghambat atau membunuh Streptococcus mutans dapat digunakan antibakteri. Salah satu tanaman herbal yang diduga memiliki aktivitas antibakteri adalah daun ceplukan (Physalis minima L.). Daun ceplukan (Physalis minima) memiliki kandungan seperti alkaloid, flavonoid, tanin dan terpenoid. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak etanol daun ceplukan (Physalis minima L) memiliki efek antibakteri terhadap Streptococcus mutans. Penelitian ini adalah suatu penelitian eksperimental dengan metode dilusi tabung. Kelompok perlakuan yaitu kelompok bakteri yang diberi ekstrak daun ceplukan (Physalis minima L.) dengan konsentrasi 5%, 7,5%, 10%, 12,5%, dan 15%. Kelompok kontrol terdiri dari kontrol bakteri sebagai kontrol positif dan kontrol ekstrak daun ceplukan (Physalis minima L.) sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa Kadar Hambat Minimum (KHM) diperoleh pada 10%, dan Kadar Bunuh Minimum (KBM) diperoleh pada 12,5%. Hasil statistik one-way ANOVA menunjukkan terdapat perbedaan signifikan antara perubahan konsentrasi ekstrak daun ceplukan terhadap Streptococcus mutans (nilai signifikansi 0,000 ; p < 0,05). Uji korelasi menunjukkan adanya hubungan kuat antara konsentrasi ekstrak dengan jumlah koloni (korelasi, r = -0,732 ; p < 0,05). Kesimpulan dari penelitian ini adalah ekstrak etanol daun ceplukan (Physalis minima L.) memiliki efek antibakteri terhadap Streptococcus mutans secara in vitro, KHM diperoleh pada konsentrasi 10% dan KBM pada konsentrasi 12,5%. Semakin tinggi konsentrasi ekstrak etanol daun ceplukan (Physalis minima L.) maka semakin rendah pertumbuhan koloni bakteri Streptococcus mutans.
English Abstract
Streptococcus mutans is a gram-positive bacteria in oral cavity which metabolism produces acid as the common cause of dental caries (tooth decay). In order to prevent the growth or kill Streptococcus mutans, antibacterial agent is required. One of the herb that suppose to have an antibacterial activity is wild gooseberry leaves (Physalis minima L.). Wild gooseberry leaves (Physalis minima L.) have contain substances such as alkaloids, flavonoids, tannins and terpenoids. This research aims to prove that the ethanolic extract of wild gooseberry leaves have antibacterial effects against Streptococcus mutans. This research is an experimental study with the tube dilution method. The treated groups are bacteria which are given wild gooseberry leaves extract with a range of concentration from 5%; 7,5%; 10%; 12,5%; 15%. The control group consisted of bacteria as a positive control and wild gooseberry leaves extract (Physalis minima L.) as a negative control. Result of this research indicated that Minimum Inhibitory Concentration (MIC) is found at concentration 10% and the Minimum Bactericidal Concentration (MBC) is found at concentration 12,5%. Results of statistical test One-way Anova showed that there was a significant difference between the ethanolic extract of wild gooseberry leaves (Physalis minima L.) concentration and colony of Streptococcus mutans (Significancy value 0,000 ; p<0,05). Correlation test showed that there was a close relation between the extract concentration and colony of Streptococcus mutans (Correlation value, r= -0,732 ; p<0,05). The conclusion of this research is ethanolic extract of wild gooseberry leaves extract (Physalis minima L.) has antibacterial effects against Streptococcus mutans in vitro, with MIC at concentration 10% and MBC at concentration 12,5%. The higher concentration of wild gooseberry leaves extract (Physalis minima L.), the less number of the colony growth.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/190/051402311 |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 14 Apr 2014 11:29 |
| Last Modified: | 21 Oct 2021 08:04 |
| URI: | http://repository.ub.ac.id/id/eprint/124162 |
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