Febrianto, Wahyu (2014) Ekstrak Daun Kemangi (Ocimum sanctum) Menghambat Peningkatan Kadar Malondialdehid Jaringan Paru pada Tikus (Rattus norvegicus) Strain Wistar yang Dipapar Asap Rokok Kretek Sub Akut. Sarjana thesis, Universitas Brawijaya.
Abstract
Menghirup asap rokok dapat menyebabkan penyakit seperti COPD, asma, bahkan kanker. Kerusakan akibat oksidan yang ada dalam asap rokok terjadi melalui mekanisme lipid peroxidation (LPO) yang mendegradasi PUFA sehingga menghasilkan produk turunan seperti malondialdehyde (MDA) yang bisa memicu kerusakan lebih lanjut dan produk turunan lain yang bersifat karsinogen seperti 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Be-berapa studi melaporkan bahwa Ocimum sanctum diketahui memiliki potensi se-bagai analgesik, antikanker, antiasma, antiemetik, dan antidiabetik. Salah satu zat yang paling dominan jumlahnya dan memiliki potensi antioksidan adalah eugenol yang bisa menjadi scavenger ROS yang memiliki satu elektron bebas dan berperan pada proses LPO. Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak daun kemangi ( Ocimum sanctum ) terhadap kadar MDA paru-paru tikus ( Rattus norvegicus ) strain Wistar dengan paparan asap rokok kretek sub akut. Tikus di bagi kedalam 5 kelompok, dimana 1 kelompok sebagai kontrol negatif dan 4 kelompok diantaranya di papar asap rokok selama 6 minggu dan 3 kelompok diantaranya diberi ekstrak daun kemangi dengan variasi dosis 100, 200, dan 400 mg/kgBB/hari. Setelah minggu ke 6, tikus dibedah untuk diambil organ paru-parunya. Kemudian jaringan paru di proses dengan metode Thiobarbituric Acid (TBA) dan kemudian dibaca dengan spektrofotometer. Hasil penelitian ini didapatkan kadar MDA yang berbeda nyata antara kelompok perla-kuan dengan kelompok kontrol positif (p<0,05). Dari uji korelasi didapatkan bahwa ada hubungan yang kuat atara pemberian ekstrak daun kemangi dengan penurunan kadar MDA jaringan paru (R=-0,545). Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak daun kemangi ( Ocimum sanctum ) mampu menghambat peningkatan kadar MDA paru-paru tikus ( Rattus norvegicus ) strain Wistar dengan paparan asap rokok kretek sub akut.
English Abstract
Inhaled cigarette smoke can suffered diseases like COPD, asthma, even a cancer. Damage because of oxidant in cigarette smoke was occured by lipid peroxidation (LPO) mechanism which can degrade Poly-Unsaturated Fatty Acid (PUFA) then produce derivate product like malondialdehyde (MDA) which can triggered further damage and other carsinogen derivate product like 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Several studies reported that Ocimum sanctum has potential as analgesic, anticancer, antiasthma, antiemetic, and antidiabetic. Eugenol that a most dominant component in tulsi has a antioxidant potential and can be as ROS scavenger that have single free electron and have a role in the process of LPO. The purspose of this study is to knowing the effect of tulsi leaf extract on lung tissue’s MDA of strain wistar rat were exposed by sub acute clove cigarette smoke. Rats divided into 5 groups where 1 group as negative control group, 4 groups were exposed by cigarette smoke during 6 weeks and 3 groups of them were given tulsi leaf extract with dosage variance was 100, 200, and 400 mg/kgBW/day. After 6 weeks, rats lungs was taken to be processed by Thiobarbituric Acid (TBA) method and then read the result by spectrophotometer. The results of this study found that MDA levels were significantly different between the groups treated with the positive control group (p <0.05). The correlation test showed that there is a strong relationship between administration of tulsi leaf extract and decreased of MDA levels in lung tissue (R = -0.545). From these results can be concluded that the tulsi ( Ocimum sanctum ) leaf extract can Inhibits the increased of strain wistar rats ( Rattus norvegicus ) lung tissue’s MDA levels with sub acute exposure to cigarette smoke.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/15/051400584 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 06 Feb 2014 10:09 |
| Last Modified: | 06 Feb 2014 10:09 |
| URI: | http://repository.ub.ac.id/id/eprint/124118 |
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