Utomo, JeffriPrasetyo (2013) Uji Efek Antimikroba Ekstrak Etanol Daun Marjoram (Origanum majorana) terhadap Methicillin Resistant Staphylococcus aureus (MRSA) secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Methicilin Resistant Staphylococcus aureus (MRSA) merupakan salah satu kasus resistensi bakteri terhadap obat antibiotik yang patut diperhatikan. Insiden infeksi bakteri MRSA ini makin meningkat pesat dengan penyebaran MRSA yang tidak hanya terbatas di rumah sakit, tetapi juga di komunitas. Sedikitnya pilihan terapi untuk mengobati infeksi Methicillin Resistant Staphylococcus aureus membuka peluang penelitian terapi MRSA menggunakan bahan alami. Daun marjoram mengandung senyawa carvacrol, linalool dan terpinenes serta berbagai komponen aktif yang dapat berperan sebagai antibakteri terhadap MRSA. Penelitian ini bertujuan untuk mengetahui efek antimikroba ekstrak etanol daun marjoram terhadap MRSA secara in vitro. Desain penelitian ini adalah desain eksperimental laboratorium. Ekstrak etanol menggunakan metode maserasi dengan pelarut etanol 96%. Metode dilusi tabung digunakan untuk mengetahui Kadar Hambat Minimal (KHM) dan Kadar Bunuh Minimal (KBM). Konsentrasi akhir ekstrak yang digunakan yaitu 0.5%, 0.625%, 0.75%, 0.875%, 1%, dan 1.125%. Pada penelitian ini KHM tidak dapat ditentukan dan KBM berada pada konsentrasi 1%. Hasil penelitian menunjukkan secara signifikan bahwa peningkatan konsentrasi ekstrak daun marjoram menyebabkan penurunan tingkat pertumbuhan MRSA. Kesimpulan dari penelitian ini adalah ekstrak etanol daun marjoram memiliki efek antimikroba terhadap MRSA secara in vitro.
English Abstract
Methicilin Resistant Staphylococcus aureus (MRSA) is one of many case of bacterial resistance which should be noted. Incidence of MRSA infection is greatly increased with its spread not only in hospital but also in community. Few of therapeutic options to treat Methicillin Resistant Staphylococcus aureus infection gives opportunities to study MRSA treatment using natural ingredients. Marjoram leaf contains compound like carvacrol, linalool and terpinenes also many other active compounds which can act as antibacterial to MRSA. This study is aimed to know antimicrobial effect of ethanol extract of marjoram leaf towards MRSA in vitro. This study is using laboratory experimental design. Ethanol extract is acquired from maceration method with 96% ethanol as solvent. Tube dilution method is used to observe Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Final extract concentration that is used in this research is 0.5%, 0.625%, 0.75%, 0.875%, 1%, and 1.125%. In this study MIC cannot be determined and MBC on extract concentration 1%. This study result shows significantly that increase of extract concentration of marjoram leaf cause decrease of MRSA growth. Conclusion of this study is that ethanol extract of marjoram leaf has antimicrobial effect towards MRSA in vitro.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2013/708/051401703 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 06 Mar 2014 10:22 |
| Last Modified: | 21 Oct 2021 04:37 |
| URI: | http://repository.ub.ac.id/id/eprint/124000 |
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