Hariyanto, Muhamad Beny (2013) Pengaruh Pemberian Buah Melon (Cucumis melo L.) Personde Terhadap Gambaran Histopatologi Zona Sentrilobular Hepar Tikus Putih (Rattus norvegicus) Galur Wistar yang Diinduksi CCl4 (Karbon Tetraklorida). Sarjana thesis, Universitas Brawijaya.
Abstract
Kerusakan organ hati merupakan salah satu penyebab kematian terbanyak di dunia disebabkan karena peroksidasi lipid dan kerusakan oksidatif, yang berkaitan erat dengan radikal bebas. Stress oksidatif menyebabkan penurunan antioksidan endogen. CCl4 (karbon tetraklorida) menyebabkan kerusakan hepar dengan mekanisme pembentukan radikal bebas dan peroksidasi lipid. Buah Melon (Cucumis melo L.) mengandung antioksidan seperti vitamin C dan β-karoten diduga mampu menangkal radikal bebas. Tujuan penelitian adalah mengetahui pengaruh jus buah melon terhadap gambaran histopatologi zona sentrilobular pada hepar tikus yang diinduksi CCl4. Studi eksperimental menggunakan Post Test Only Control Group dilakukan pada 25 hewan coba tikus putih wistar jantan (Rattus Novergicus) yang dibagi menjadi lima kelompok terdiri dari kontrol negatif (normal), kontrol positif (diinduksi CCl4) dan tiga kelompok perlakuan (diinduksi CCl4 dan dilanjutkan pemberian jus melon masing-masing 2ml/hari, 4ml/hari, 6 ml/hari). Variabel yang diukur adalah nekrosis sel hepar (metode pewarna Hematoxylin Eosin). Hasil penelitian didapatkan sel hepar mengalami nekrosis signifikan akibat induksi CCl4. Semua dosis jus melon dapat menurunkan nekrosis sel hepartikus, terutama dosis 6ml/hari yang mampu menurunkan nekrosis sel hepar secara signifikan (p=0.000). Kesimpulan penelitian adalah pemberian jus buah melon (Cucumis melo L.) dapat menurunkan jumlah nekrosis sel hepar pada zona sentrilobular tikus putih galur wistar yang diinduksi CCl4 dan dosis efektifnya adalah 6 ml/hari.
English Abstract
Liver damage is one of the most frequent death cause in the world,that iscaused by lipid peroxidation and oxidative damage which is related strongly tofree radicals. Stress oxidative decrease of antioxidant endogenous activity. CCl4 (Carbon tetrachloride) can induce liver damage through formation of free radicals and lipid peroxidation mechanism. Melon fruit (Cucumis melo L.) contains antioxidant such as vitamin C and β-carotene which are presumed for having free radicals scavenging action. This study aims to determine the effect of melon fruit juice on superoxide dismutase (SOD) enzyme activity on liver rats induced CCl4. Experimental study using Post Test Only Control Group design divided 25 male rats wistar strain (Rattus novergicus) into five groups, that were negative control (normal), positive control (induced CCl4) and three groups treatment (induced CCl4 and treated with melon juice 2ml/day, 4 ml/day, 6ml/day for every group). The variable measured was hepatic cell necrosis (Hematoxylin eosin staining). This study show that CCL4induce necrosis in liver cell significantly and all doses of melon juice can lower rats hepatic cell necrosis, especially on dose 6ml/day which can reduce hepatic necrosis significantly (p=0.000). The conclusion is giving fruit juice melon (Cucumis melo L.) can reduce centrilobular zone hepatic cell necrosis in the wistar rats induced by CCl4 and effective dose is 6 ml/day.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2013/425/051305829 |
| Uncontrolled Keywords: | buah melon,karbon tetraklorida, zona sentrilobular hati; melon fruit, Carbon tetrachloride,centilobular zone of the liver |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.3 Diseases of digestive system > 616.36 Diseases of biliary tract and liver > 616.362 Diseases of liver /Liver--Diseases |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Hasbi |
| Date Deposited: | 30 Jul 2013 09:36 |
| Last Modified: | 02 Dec 2021 04:52 |
| URI: | http://repository.ub.ac.id/id/eprint/123708 |
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