Ismail, Arif (2013) Pengaruh Induksi Cathepsin K terhadap Pembentukan Immunoglobuline G (IgG)Anti-Cathepsin K, Jumlah Osteosit dan Kadar Alkaline Phosphatase (ALP) pada Tikus Rattus Norvegicus Wistar Betina Post-Ovariekt. Sarjana thesis, Universitas Brawijaya.
Abstract
Osteoporosis adalah suatu "silent disease" yang dapat melemahkan tulang dan menyebabkan fraktur. Dua dari lima penduduk Indonesia berisiko terkena osteoporosis dan diperkirakan pada tahun 2025 angka tersebut meningkat tiga kali lipat. Saat ini telah ditemukan obat-obatan yang berfungsi sebagai inhibitor cathepsin K yang menunjukkan potensi besar dalam menurunkan tingkat osteoporosis. Cathepsin K berperan penting dalam destruksi jaringan, remodelling , dan perusakan kartilago tulang. Penelitian ini ditujukan untuk membuktikan pengaruh pemberian kandidat vaksin berbahan dasar cathepsin K terhadap penurunan kecepatan resorbsi tulang pada tikus Wistar ( Rattus norvegicus ) yang diovariektomi. Tikus putih Wistar betina berusia 10-12 minggu dikelompokkan menjadi 5 kelompok: kontrol (-), kontrol (+) yang diovariektomi, kelompok perlakuan yang diovariektomi dan diberikan cathepsin K 50 ng/200 μL, 100 ng /200 μL, dan 200 ng/200 μL. Pembedahan dilakukan pada hari ke-30 kemudian dilakukan pengukuran titer IgG anti- cathepsin K dan penghitungan jumlah osteosit. Uji ANOVA menunjukkan bahwa pemberian kandidat vaksin cathepsin K yang ditambahkan dengan CFA-IFA secara bermakna meningkatkan titer IgG anti- cathepsin K dalam serum (p=0,00). Pemberian cathepsin K dosis 50 ng/200 μL, 100 ng /200 μL, dan 200 ng/200 μL tidak mengurangi jumlah osteosit secara bermakna. Pemberian cathepsin K dosis 50 ng/200 μL, 100 ng /200 μL, dan 200 ng/200 μL tidak meningkatkan ALP serum (p>0,05) secara bermakna. Kesimpulan penelitian adalah bahwa pemberian kandidat vaksin cathepsin K dapat meningkatkan titer IgG anti- cathepsin K pada tikus putih ( Rattus norvegicus ) galur Wistar yang di ovariektomi, tetapi tidak berpengaruh secara bermakna terhadap jumlah osteosit dan kadar ALP serum. Oleh karena itu, kandidat vaksin osteoporosis dengan bahan dasar cathepsin K masih perlu diteliti dan dikembangkan lebih lanjut lagi.
English Abstract
Osteoporosis is a "silent disease" that can weaken bones and cause fractures. Two from five of Indonesias population at risk of osteoporosis and it is estimated in 2025 that number will become tripled. It has been found a kind of drug, that serves as cathepsin K inhibitors, that shows great potential in reducing osteoporosis. Cathepsin K has an important role in tissue destruction, bone remodeling, and cartilage destruction. This study is aimed to verify the effect of the cathepsin K vaccine candidate to the decrease of bone resorbtion in Rattus norvegicus strain wistar post-ovariectomy. Female Rattus norvegicus Strain Wistar (age: 10-12 weeks) are divided into 5 groups: control (-), control (+) with ovariectomy, the treatment groups are ovariectomized and given cathepsin K 50 ng/200 μL, 100 ng /200 μL, and 200 ng/200 μL. Surgery perform on the 30th day and then IgG titers of anti-cathepsin K and ALP serum level are measured and the number of osteocytes is counted. ANOVA test shows that the administration of the vaccine candidate, cathepsin K that are added with CFA-IFA, is significantly increase the titers of IgG anti-cathepsin K in the serum (p = 0.00). The administration of cathepsin K 50 ng/200 μL, 100 ng /200 μL, and 200 ng/200 μL do not significantly reduce the number of osteocytes. The administration of cathepsin K 50 ng/200 mL, 100 ng / 200 mL, and 200 ng/200 mL do not increase the ALP serum levels (p> 0.05) significantly. The conclusion of the study is that the administration of the cathepsin K vaccine candidate can increase the IgG anti-cathepsin K titers in Female Rattus norvegicus Strain Wistar Rats Post-ovariectomy , but do not significantly affect the number of osteocytes and ALP serum levels. Therefore, the osteoporosis vaccine candidate with cathepsin K as a basic material still need to be researched and developed further.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2013/33/051301944 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 05 Mar 2013 16:28 |
| Last Modified: | 31 Dec 2021 03:37 |
| URI: | http://repository.ub.ac.id/id/eprint/123610 |
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