Rada, AnnisaA (2012) Efek Ekstrak Etanol Umbi Rumput Teki (Cyperus rotundus) terhadap Jumlah Foam Cell pada Tikus Wistar Jantan yang Diberi Diet Aterogenik. Sarjana thesis, Universitas Brawijaya.
Abstract
Berdasarkan penelitian WHO diperoleh data bahwa 20% kasus kematian di seluruh dunia diakibatkan oleh penyakit yang didasari oleh aterosklerosis. Terbentuknya foam cell merupakan awal dari timbulnya aterosklerosis. Antioksidan seperti polifenol menjadi salah satu metode mencegah aterosklerosis. Salah satu tanaman yang mengandung kandungan tinggi polifenol adalah umbi rumput teki ( Cyperus rotundus ). Penelitian ini bertujuan untuk mengetahui bahwa pemberian ekstrak umbi rumput teki berpengaruh terhadap jumlah foam cell di subendotel aorta tikus Wistar yang diberi diet aterogenik. Studi ini menggunakan metode randomized post test only controlled group design . Sampel dipilih secara acak kemudian dibagi menjadi 5 kelompok yakni kelompok kontrol negatif (diet normal), kelompok kontrol positif (diet aterogenik), dan tiga kelompok yang diberikan diet aterogenik ditambah ekstrak umbi rumput teki dengan dosis 6.14 mg/hari, 12.28 mg/hari, dan 24.56 mg/hari selama 60 hari. Pemberian diet dan ekstrak dilakukan selama 60 hari. Variabel yang diukur adalah jumlah foam cell pada subendotel aorta. Hasil yang diperoleh pada kelompok perlakuan 3 (24.56 mg/hari) menunjukkan jumlah foam cell paling minimal dibanding kelompok perlakuan lain. Hasil penelitian menunjukkan rerata jumlah foam cell kelompok kontrol negatif sebesar 1,4 ±1,516575, kelompok kontrol positif sebesar 16 ± 3,937004, kelompok perlakuan 1 (6.14 mg/hari) sebesar 4,4 ± 0,547723, kelompok perlakuan 2 (12.28 mg/hari) sebesar 2,8 ±3,271085, dan kelompok perlakuan 3 (24.56 mg/hari) sebesar 1,2 ±2,167948. Hasil uji One Way ANOVA menunjukkan adanya perbedaan signifikan antar kelompok (p<0.05). Kesimpulan dari penelitian ini adalah pemberian ekstrak umbi rumput teki mampu menghambat proses pembentukan foam cell di subendotel aorta pada tikus Wistar yang diberi diet aterogenik secara signifikan, dengan dosis 24.56 mg/hari.
English Abstract
According to the data derived from the studies by WHO, it was shown that 20% of deaths worldwide result from deseases caused by atherosclerosis.The formation of foam cell is the beginning of the onset of atherosclerosis. Antioxidant such as polyphenol is one of atherosclerosis prevention methods. One of the plants that contain high level of polyphenol are nut grass ( Cyperus rotundus ) rhizome. The purpose of this study was to prove whether nut grass rhizome extract could inhibit the foam cell formation process in wistar rats fed with atherogenic diet. This experimental study used randomized post test only controlled group design . The subjects were randomly selected and divided into five groups. The groups were negative control (normal diet), positive control (atherogenic diet), and three test groups that were given atherogenic diet added with 6.14; 12.28; and 24.56 mg/day nut grass rhizome extract for 60 days. The measured variable was number of foam cells in subendothel aorta. The results of this study indicate that the number of foam cells of dose group 3 (24.56 mg/day) had the lowest number of foam cells compared to the other dose groups. The obtained mean of number of foam cells of negative control group was 1,4 ±1,516575; positive control group was 16 ± 3,937004; dose group 1 (6.14 mg/day) was 4,4 ± 0,547723; dose group 2 (12.28 mg/day) was 2,8 ±3,271085 and; dose group 3 (24.56 mg/day) was 1,2 ±2,167948. One Way ANOVA test showed a significant differences among all groups (p<0,05). It is concluded that nut grass ( Cyperus rotundus) rhizome extract can significantly inhibit foam cell formation process on wistar rats fed with atherogenic diet, at the dose of 24.56 mg/day.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2012/417/051301939 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 11 Mar 2013 15:37 |
| Last Modified: | 19 Oct 2021 03:27 |
| URI: | http://repository.ub.ac.id/id/eprint/123342 |
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