Hidayatullah, Furqan (2014) Efek Antibodi Telomerase Pada Sel Kanker Leher Rahim : Pendekatan Secara Biomolekular. Sarjana thesis, Universitas Brawijaya.
Abstract
Kanker leher rahim merupakan salah satu kanker dengan angka kematian tertinggi di Indonesia. Hingga saat ini belum ditemukan pengobatan yang adekuat pada kanker leher rahim. Telomerase merupakan molekul yang terdapat pada kanker leher rahim. Ditemukan aktifitas telomerase lebih dari 95% pada kanker leher rahim. Telomerase tidak ditemukan pada sel normal, telomerase berperan sangat aktif dalam pembelahan sel kanker. Pengobatan kanker leher rahim melalui penghambatan protein telomerase diharapkan dapat menjadi suatu terapi yang spesifik dan memberikan efek samping yang minimal pada sel normal. Tujuan penelitian ini adalah menghasilkan antibodi poliklonal dan mengujicobakanya pada sel HeLa. Produksi antibodi menggunakan vektor kelinci, antibodi yang dihasilkan kelinci dipurifikasi menggunakan metode SAS. Antibodi selanjutnya dideteksi menggunakan metode Western Blotting dan ELISA. Antibodi diujicobakan pada sel HeLa dan diamati menggunakan immunositokimia dan aktifitas proliferasinya diamati menggunakan MTT assay. Hasilnya terdapat pita pada western blotting pada berat molekul 58 Kda dan juga hasil ELISA menunjukan adanya kesamaan titer antibodi dengan konsep immunologi. Immunositokimia menunjukan adanya peningkatan ikatan antigen antibodi setiap kali dosis antibodi ditingkatkan. Hasil MTT assay menunjukan terdapat penurunan aktifitas proliferasi sel HeLa pada dosis optimum 2 µL dengan p=0.032. kesimpulan dari penelitian ini adalah antibodi telomerase berpotensial untuk dikembangkan sebagai suatu pengobatan kanker leher rahim.
English Abstract
Cervical cancer is one of the most deadly cancer in Indonesia, it has the highest mortality rate among others cancers. Cervical cancer therapy has not been found. Telomerase is known as one of the most important molecule in cervical cancer, it is found more than 95% in cervical cancer cell. However,telomerase is not found in normal human cell. Telomerase is known as a molecule that has some critical rules in cell proliferation. Cervical cancer treatment based on telomerase inhibition is believed to be one of the best solution to treat cervical cancer beside of that telomerase is not found in normal human cell therefore it can minimize the side effect of cancer treatment. This research are aims to produce polyclonal telomerase antibody and to see if there is any effect of telomerase antibody in Hela cell culture. Antibody was produce using two rabbits, rabbit’s serum was collected and purified using SAS (Saturrated ammonium sulfate). Antibody was detected using western blotting and ELISA. Telomerase antibody effect in hela cell line was observed using immunocytochemistry and MTT assay method. The result are there is a band shows in 58 kda using western blotting, ELISA shows same graphic of telomerase antibody titer and immunology titer. Immunocytochemistry shows there is an increasases binding site oftelomerase and telomerase antibody in hela cell as many as telomerase antibody added. MTT assay shows there is a decreases number of hela cell proliferation as many as antibody doses increases. MTT assay suggest that hela cell’s proliferation is significantly decreased at 2µl of antibody dose(p= 0.032). This research suggest that telomerase antibody has a potention to be developed as human cervical cancer treatment.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2010/411/051402191 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Hasbi |
| Date Deposited: | 01 Apr 2014 11:01 |
| Last Modified: | 22 Oct 2021 07:56 |
| URI: | http://repository.ub.ac.id/id/eprint/123226 |
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