Wahjuningsih, Sri and Djati, M. Sasmito (2007) Perubahan Struktur Sitoskeleton Berbasis Mikrotubulus dan Ultrastruktur Oosit Pasca Kriopreservasi dengan Metode Vitrifikasi. Project Report. Fakultas Peternakan Universitas Brawijaya, Malang. (Unpublished)
Abstract
Tujuan penelitian adalah mengkaji ultrastruktur oosit pasca vitrifikasi menggunakan konsentrasi EG 30 % dan lama paparan 3 menit menggunakan TEM (Transmisi Elektron Mikroskop). Analisa data secara deskriptif. Hasil penelitian menunjukkan oosit hasil vitrifikasi mempunyai zona pelusida yang abnormal (fraktur), membran plasma lapis ganda mengalami lisis, beberapa butir korteks mengalami degenerasi dengan derajat yang terlihat dan perpindahan butir korteks di ruang perivitelin. Kesimpulan dari penelitian ini adalah terdapat perubahan ultrastruktur pada oosit setelah vitrifikasi menggunakan EG 30 % dengan lama paparan 3 menit.
English Abstract
The aim of this research for the second year was to analilyzed ultrastructural of cryopreserved oocyte using vitrification method by Transmission Electrone Microscope (TEM). The results of the research showed that cryopreserved oocyte using vitrification had ultrastructural change : zona fracture, lysis of membrane plasm, change of cortical granule position to per vitelin space and degeneration of cortical granule. The conclusion of this research was that vitrification use 30% EG and the time of exposure during 3 minutes caused ultrastructural change in goat oocyte.
| Item Type: | Monograph (Project Report) |
|---|---|
| Identification Number: | PEN/571.654/WAH/p/2007/020800495 |
| Uncontrolled Keywords: | vertfication,ultrastructural,goat oocyte |
| Subjects: | 500 Natural sciences and mathematics > 571 Physiology and related subjects > 571.6 Cell biology > 571.65 Cytoplasm > 571.654 Cytoskeleton |
| Divisions: | Fakultas Peternakan > Peternakan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 13 Sep 2018 07:15 |
| Last Modified: | 16 Jun 2022 07:50 |
| URI: | http://repository.ub.ac.id/id/eprint/11974 |
![[thumbnail of 020800495-ok.pdf]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text
020800495-ok.pdf Restricted to Registered users only Download (3MB) |
Actions (login required)
 |
View Item |