Sari, Syintia Indah Puspita (2018) Perbanyakan Bibit Bawang Dayak (Eleutherine Palmifolia L.) Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Bawang dayak (Eleutherine palmifolia L.) merupakan jenis tanaman yang dikategorikan dalam kelompok tanaman obat atau herbal. Tanaman bawang dayak telah lama dikenal masyarakat lokal sebagai obat berbagai jenis penyakit. Umbi bawang dayak mempunyai kandungan senyawa fitokimia seperti alkaloid, glikosida, flavonoid, fenolik, steroid dan tannin yang dimanfaatkan sebagai obat herbal seperti kanker payudara, obat penurun darah tinggi (hipertensi), penyakit kencing manis (diabetes melitus), menurunkan kolesterol, obat bisul, kanker usus dan mencegah stroke. Tanaman bawang dayak memiliki potensi besar untuk dikembangkan dalam skala industri. Namun dalam skala besar perbanyakan bawang dayak masih terbatas dalam penyediaan bibit. Oleh karena itu dibutuhkan upaya dalam perbanyakan bawang dayak dalam skala besar yaitu melalui teknik kultur jaringan. Zat pengatur tumbuh yang dapat digunakan untuk keberlangsungan kultur jaringan yakni auksin (NAA) dan sitokinin (BAP). Tujuan dari penelitian ini adalah mempelajari dan mendapatkan kombinasi konsentrasi NAA dan BAP yang tepat untuk perbanyakan eksplan bawang dayak secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya. Penelitian dilaksanakan dari bulan Agustus – November 2017. Alat yang digunakan dalam penelitian adalah timbangan analitik, pH meter, magnetik stirer, pengaduk, autoclave, shaker, gelas ukur, petridish, scalpel, pinset, pipet tetes, pipet hisab, spatula, gelas erlenmeyer, gunting, lampu Burnsen, Laminar Air Flow Cabinet (LAFC), microwave, cawan petri, rak kultur, beaker glass, handsprayer, penggaris, botol kultur, oven, karet gelang, plastik, tisu, kompor, alat tulis dan kamera. Bahan yang digunakan meliputi eksplan tunas bawang dayak, komposisi media Murashige dan Skoog (MS), casein 0,08 g/L, zat pengatur tumbuh paclobutrazol, NAA dan BAP, tisu, alkohol 70 % dan 96 %, HCL 1 N, NaOH 1 N, clorox 15 %, agar 6,3 g, sukrosa 30 g, label, aquades, fungisida (blanlate) 5 g/l, bakterisida (streptomycin) 5 g/l , detergen, dan spiritus.Penelitian menggunakan Rancangan Acak Lengkap (RAL) sederhana dengan 4 ulangan. Faktor yang digunakan adalah kontrol; NAA 0,1 + BAP 0,5; NAA 0,2 + BAP 0,1; NAA 0,3 + BAP 1,5; NAA 0,4 + BAP 2 ; NAA 0,5 + BAP 2,5; sehingga terdapat 6 perlakuan. Setiap perlakuan terdiri dari 6 botol kultur dengan jumlah 1 eksplan dan sampel diambil sebanyak 4 botol per perlakuan. Pengamatan yang diamati meliputi saat muncul tunas, saat muncul akar, jumlah tunas, jumlah akar, tinggi eksplan, jumlah daun dan presentase eksplan hidup. Data jumlah akar dan jumlah daun di transformasi menggunakan transformasi akar [(x+0,5)]. Data pengamatan yang diperoleh dianalisis menggunakan analisis ragam (uji F) pada taraf 5 %. Apabila hasil uji diperoleh pengaruh perlakuan yang nyata maka dilanjutkan dengan uji BNT pada taraf 5%. ii Hasil penelitian dapat diketahui bahwa perlakuan dengan dengan konsentrasi auksin (NAA 0,1 ppm) dan sitokinin (BAP 0,5 ppm) mampu menunujukkan waktu muncul tunas paling cepat yaitu 21,57 hari setelah inokulasi. Sedangkan pada induksi tunas adalah media dengan perlakuan NAA 0,3 ppm dan BAP 1,5 ppm mampu menghasilkan jumlah tunas sebanyak 1,40
English Abstract
Dayak onions (Eleutherine palmifolia L.) is a type of plant that is categorized in a group of medicinal plants or herbs. Dayak onion plants have long been known to the local community as a cure for various types of diseases. Dayak bulbs contain phytochemical compounds such as alkaloids, glycosides, flavonoids, phenolics, steroids and tannins used as herbal remedies such as breast cancer, high blood pressure (hypertension), diabetes (diabetes mellitus), lowering cholesterol, colon cancer and prevent stroke. Dayak onion plants have great potential to be developed on an industrial scale. However, in large-scale dayak onions propagation is still limited in the supply of seeds. Therefore it takes effort in propagation of dayak onions on a large scale through tissue culture techniques. Growth regulator substances that can be used for the continuity of tissue culture namely auksin (NAA) and cytokinin (BAP). The purpose of this study is to learn about and get the combination of the concentration of NAA and BAP for proper reproduction eksplan dayak onion in vitro. The research was carried out in the Culture Tissue Laboratory of the Department of Agriculture Cultivation Faculty of Agriculture Universitas Brawijaya. The research was carried out from August to November 2017. Tools used in the study was analytical scales, pH meters, magnetic stirers, stirrers, autoclaves, shakers, measuring cups, petridish, scalpel, tweezers, dropper pipettes, pipettes, spatulas, erlenmeyer glasses, scissors, Burnsen lamps, Laminar Air Flow Cabinet (LAFC), microwave, petri dish, culture rack, beaker glass, handsprayer, ruler, culture bottle, oven, rubber band, plastic, tissue, stove, stationery and camera. The ingredients used included the explants of Dayak onion shoots, Murashige and Skoog (MS) media compositions, 0.08 g / L casein, paclobutrazole growth agent, NAA and BAP, tissue, 70% and 96%, HCL 1N, NaOH 1 N, clorox 15%, agar 6.3 g, sucrose 30 g, label, aquades, fungicide (blanlate) 5 g/l, bactericide (streptomycin) 5 g/l, detergent, and spiritus. Research using Randomized Complete Design (RAL) with 4 replicates. Factor used is control; NAA 0,1 + BAP 0.5; NAA 0,2 + BAP 0,1; NAA 0,3 + BAP 1,5; NAA 0,4 + BAP 2; NAA 0.5 + BAP 2.5; so there are 6 treatments. Each treatment consisted of 4 culture bottles with 1 explant amount and 4 bottles taken each treatment. Observations observed include when shoots appear, when the roots appear, the number of shoots, the number of roots, explant height, number of leaves and live explant percentage. The data on the number of roots and the number of leaves in the transformation using the root transformation [(x+0,5)]. The observed data were analyzed using variance analysis (F test) at 5% level. If the test results obtained the effect of real treatment then followed by BNT test at 5% level. The result showed that treatment with auksin concentration (NAA 0,1 ppm) and cytokinin (BAP 0,5 ppm) was able to show the fastest buding time that was 21,57 days after inoculation. iv While on shoot induction is a medium with NAA treatment of 0.3 ppm and 1.5 ppm BAP able to produce the number of shoots as much as 1.40.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/127/051802602 |
| Uncontrolled Keywords: | - |
| Subjects: | 600 Technology (Applied sciences) > 635 Garden crops (Horticulture) > 635.2 Edible tubers and bulbs > 635.25 Onions |
| Divisions: | Fakultas Pertanian > Budidaya Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 04 Jun 2018 02:58 |
| Last Modified: | 20 Oct 2021 04:12 |
| URI: | http://repository.ub.ac.id/id/eprint/11330 |
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E. BAB IV.pdf Download (264kB) | Preview |
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F. BAB V.pdf Download (85kB) | Preview |
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G. DAFTAR PUSTAKA.pdf Download (183kB) | Preview |
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H. LAMPIRAN.pdf Download (613kB) | Preview |
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A. BAGIAN DEPANN.pdf Download (394kB) | Preview |
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B. BAB I.pdf Download (90kB) | Preview |
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D. BAB III.pdf Download (206kB) | Preview |
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C. BAB II.pdf Download (415kB) | Preview |
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