Wardhani, Untari Prawita (2018) Efektivitas Rhizobium Endogen Pada Tanah Bekas Penanaman Kedelai Terhadap Pertumbuhan Enam Varietas Kedelai (Glycine Max L.). Sarjana thesis, Universitas Brawijaya.
Abstract
Rhizobium adalah bakteri yang dapat mengikat nitrogen dari alam dan menyediakan nitrogen bagi tanaman. Akan tetapi populasi Rhizobium dalam tanah dapat terdiri dari bakteri yang tidak efektif hingga sangat efektif. Penelitian ini bertujuan untuk mengetahui pengaruh sifat kimia dari tanah bekas penanaman kedelai terhadap populasi Rhizobium endogen dari tanah bekas penanaman kedelai dan pengaruh Rhizobium endogen dari tanah bekas penanaman kedelai terhadap nodulasi akar dan pertumbuhan dari enam varietas tanaman kedelai. Penelitian ini dilaksanakan pada bulan Juni hingga Oktober 2017. Penanaman dilaksanakan di rumah kaca Balai Penelitian Tanaman Aneka Kacang dan Umbi (Balitkabi). Analisis variabel pengamatan dilaksanakan di Laboratorium Kimia Tanah, Fakultas Pertanian, Universitas Brawijaya dan Laboratorium Mikrobiologi Tanah Balitkabi. Rancangan penelitian menggunakan Rancangan Acak Kelompok (RAK) sederhana yang terdiri dari 12 kombinasi perlakuan dan tiga ulangan sehingga terdapat 36 unit perlakuan. Kombinasi perlakuan yaitu terdiri dari, T1V1 (Tanah non steril + varietas Argomulyo), T1V2 (Tanah non steril + varietas Burangrang), T1V3 (Tanah non steril + varietas Grobogan), T1V4 (Tanah non steril + varietas Dering I), T1V5 (Tanah non steril + varietas Devon I), T1V6 (Tanah non steril + varietas Dega I), T2V1 (Tanah steril + varietas Argomulyo), T2V2 (Tanah steril + varietas Burangrang), T2V3 (Tanah steril + varietas Grobogan), T2V4 (Tanah steril + varietas Dering I), T2V5 (Tanah steril + varietas Devon I), T2V6 (Tanah steril + varietas Dega I). Data dianalisis dengan menggunakan sidik ragam (ANOVA) dengan taraf 5% dan apabila berpengaruh nyata dilakukan uji lanjut menggunakan uji Duncan Multiple Range Test (DMRT) dengan taraf 5% dan dilakukan uji korelasi untuk mengetahui hubungan antar variabel. Perlakuan tanah non steril dengan varietas Dering I (T1V4) menunjukkan hasil yang terbaik dengan nilai C-organik sebesar 1,67%, pH tanah sebesar 5,89, N-total tanah sebesar 0,172% dan populasi Rhizobium sebesar 89,00 x 109 cfu ml-1. Perlakuan tanah non steril dengan varietas Dering I (T1V4) menunjukkan hasil yang terbaik terhadap infektivitas dengan jumlah bintil sebanyak 80 butir tan-1 dan bobot bintil sebesar 0,73 g tan-1 serta efektivitas dengan serapan N tanaman sebesar 14,23 g tan-1, tinggi tanaman (42 HST) 39,83 cm dan kadar klorofil (42 HST) sebesar 41,43 unit. Populasi Rhizobium menunjukkan hubungan yang sangat kuat dengan jumlah bintil (r=0,86), serapan N tanaman (r=0,87), tinggi tanaman (r=0,96), kadar klorofil (r=0,91), C-organik (r=0,97), pH (r=0,89) dan N-total (r=0,88). Populasi Rhizobium menunjukkan hubungan yang kuat dengan bobot bintil (r=0,75).
English Abstract
Rhizobium is bacterium that can fixed nitrogen from nature and supplied nitrogen for plants. However, population of Rhizobium in the soil may consist of ineffective strains to very effectively. This study purpose to understand the effected of soil chemical properties on endogenous Rhizobium populations from former soil of soybean planting and the endogenous Rhizobium from former soil of soybean planting of soybean effected on root nodulation and growth of six varieties of soybean plants. This study conducted on June to October 2017. Cultivation held in the green house of Balai Penelitian Tanaman Aneka Kacang dan Umbi (Balitkabi). The observation variables analysist held in Laboratory of Chemistry at Faculty of Agriculture, Brawijaya University and Laboratory of Soil Microbiology at Balitkabi. This study was using simple Completely Randomize Block Design consists of 12 treatment combinations with 3 replication that occur in 36 treatments. The treatment combinations consist of T1V1 (Non sterile soil + Argomulyo varieties), T1V2 (Non sterile soil + Burangrang varieties), T1V3 (Non sterile soil + Grobogan varieties), T1V4 (Non sterile soil + Dering I varieties), T1V5 (Non sterile soil + Devon I varieties), T1V6 (Non sterile soil + Dega I varieties), T2V1 (Sterile soil + Argomulyo varieties), T2V2 (Sterile soil + Burangrang varieties), T2V3 (Sterile soil + Grobogan varieties), T2V4 (Sterile soil + Dering I varieties), T2V5 (Sterile soil + Devon I varieties), T2V6 (Sterile soil + Dega I varieties). The data analysis was using analysist of variance at 5% significant and Duncan Multiple Range Test (DMRT) and conducted a correlation test to determine the relationship between variables. The non steril soil with Dering I varieties treatment (T1V4) showed the best result of C-organic is 1,67%, soil pH is 5,89, soil N-total is 0,172% and Rhizobium population is 89,00 x 109 cfu ml-1. The non steril soil with Dering varieties (T1V4) showed the best result of infectivity with the nodule number is 80 g plant-1 and nodule weight is 0,73 g plant-1 and effectivity with N plant uptake is 14,23 g plant-1, plant height (42 DAP) 39,83 cm and chlorophyll content (42 DAP) is 41,43 unit. Population of Rhizobium showed a very strong association with the number of nodules (r=0,86), N plant uptake (r=0,87), height of plant (r=0,96), chlorophyll content (r=0,91), C-organic (r=0,97), pH (r=0,89) and N-total (r=0,88). Population of Rhizobium showed a strong association with the weight of nodules (r=0,75).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/59/051802145 |
| Uncontrolled Keywords: | - |
| Subjects: | 600 Technology (Applied sciences) > 633 Field and plantation crops > 633.3 Legumes, forage crops other than grasses and legumes > 633.34 Soybeans |
| Divisions: | Fakultas Pertanian > Ilmu Tanah |
| Depositing User: | Nur Cholis |
| Date Deposited: | 21 May 2018 03:50 |
| Last Modified: | 20 Oct 2021 02:13 |
| URI: | http://repository.ub.ac.id/id/eprint/10745 |
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