Duwiri, Sirapegi Ockhtacia Winurai Latip (2019) Pengaruh Pemberian Single Dose Indometasin Dengan Perbedaan Waktu Terhadap Ekspresi Tnfα Dan Gambaran Histopatologi Kolon Tikus Putih (Rattus Norvegicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Inflammatory Bowel Disease (IBD) adalah spektrum dari kondisi radang idiopatik kronis yang menyebabkan gejala-gejala gastrointestinal yang signifikan termasuk diare, sakit perut, pendarahan, anemia dan penurunan berat badan. Dalam percobaan eksperimental, Indometasin sering digunakan sebagai inisiator IBD pada hewan coba. Indometasin bekerja dengan menghambat cyclooxygenase 1 (COX-1) yang berperan dalam pembentukan prostaglandin pada saluran cerna. Indometasin menghasilkan metabolit imunokuinon yang sangat reaktif yang menyebabkan stress oksidatif yang mengikis mukosa gaster dan menyebabkan pelepasan epitel, erosi, ulserasi sampai pendarahan pada gaster. Tujuan penelitian ini untuk mengetahui hasil dari pemberian indometasin dengan perbedaan waktu terhadap ekspresi TNFα dan gambaran histopatologi organ kolon tikus putih. Penelitian ini menggunakan tikus putih berumur 8 minggu dengan berat 150 gram sebanyak 20 ekor, yang dibagi menjadi empat kelompok, yaitu kelompok kontrol negatif, perlakuan 1, perlakuan 2, dan perlakuan 3. Dosis Indometasin sebanyak 3 mg/kg BB dengan sonde oral selama 33 jam, 48 jam, dan 57 jam. Metodee penelitian menggunakan Postest Only Control Design dengan Rancangan Acak Lengkap. Ekspresi TNF α diukur dengan Imunohistokimia (IHK) dan gambaran histologi kolon dengan pewarnaan Hematoksilin Eosin (HE). Analisa data ekspresi TNFα menggunakan One Way ANOVA (a=0,005) serta gambaran histologi kolon secara deskriptif. Hasil penelitian menunjukan bahwa pada pemberian Indometasin dengan perbedaan waktu secara signifikan (p<0,05) meningkatkan ekspresi TNFα dan memperparah kerusakan jaringan kolon, berupa nekrosis dan inflamasi berdasarkan gambaran histopatologi sesuai dengan peningkatan variasi waktu pemberian indometasin. Kesimpulan pemberian single dose Indometasin meningkatkan ekspresi TNFα dan memperparah kerusakan jaringan kolon berdasarkan lamanya waktu pengamatan
English Abstract
Inflammatory Bowel Disease (IBD) is a spectrum of chronic idiopathic inflammatory conditions that cause significant gastrointestinal symptoms including diarrhea, abdominal pain, bleeding, anemia and weight loss. In experimental experiments, Indomethacin is often used as an initiator of IBD in experimental animals. Indomethacin works by inhibiting cyclooxygenase 1 (COX-1) which plays a role in the formation of prostaglandins in the gastrointestinal tract. Indomethacin produces highly reactive immunocuinone metabolites which cause oxidative stress that erodes the gastric mucosa and causes epithelial release, erosion, ulceration and gastric bleeding. The purposes of this study were to determine the results of indomethacin administration with time differences in TNFα expression and histopathology of white Rat’s colon. This study used 8 weeks old white rats weighed 150 grams as many as 20 rats, which were divided into four groups, namely negative control group, treatment 1, treatment 2, and treatment 3. Indomethacin dose of 3 mg / kg Body Weight with oral sonde for 33 hours, 48 hours and 57 hours. The method of research uses Postest Only Control Design with Completely Randomized Design. TNF α expression was measured by immunohistochemistry (IHC) and histology of colon with hematoxylin eosin (HE) staining. Data analysis of TNFα expression using One Way ANOVA (a = 0.005) and descriptive histology of colon. The results showed that the administration of Indomethacin with a significant time difference (p <0.05) increased the expression of TNFα and made more severe of colon’s tissue damage, show with necrosis and Inflamation base on histopathological features in accordance with increased variation of the time of indometasin administration. The conclusion were that administration of single dose Indometachin with increased administration time could increased the expression of TNFα and the damage to colon tissue according elevation of observation time.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/125/051909055 |
| Uncontrolled Keywords: | Indometasin, TNFα, histopatologi kolon, Indomethacin, TNFα, colon histopathology |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.085 Feeds and applied nutrition > 636.085 2 Applied nutrition |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 05:54 |
| Last Modified: | 21 Oct 2021 04:22 |
| URI: | http://repository.ub.ac.id/id/eprint/176762 |
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