Nsurwakhid, Della Wachdatul Angela (2017) Optimalisasi Peningkatan Purifikasi Protein Rekombinan Ag38 Kda Mycobacterium Tuberculosis Melalui Metode Imac. Sarjana thesis, Universitas Brawijaya.
Abstract
Mycobacterium tuberculosis memiliki antigen 38 kDa yang merupakan lipoglikoprotein dengan phosphate transport protein (PSTS-1). PSTS-1 ini sebagai reseptor awal transport aktif protein. Antigen 38 kDa juga dapat digunakan sebagai pembeda antara infeksi murni Mycobacterium tuberculosis dengan spesies Mycobacterium yang lain. Oleh karena itu, Ag38 kDa merupakan kandidat potensial sebagai agen serodiagnostik. Di Laboratorium Biomedik Fakultas Kedokteran Universitas Brawijaya telah dilakukan cloning dan ekspresi gen pab pengode Ag38 kDa Mycobacterium tuberculosis. Sebelumnya telah dilakukan purifikasi dengan metode IMAC(Immobilized Metal Affinity Chromatography) kit Protino. Namun hasil purifikasi kurang memuaskan karena masih banyak protein-protein kontaminan. Pada studi ini, kami melakukan purifikasi protein rekombinan Ag38 kDa menggunakan metode IMAC kit Thermo Hispur Ni-NTA. Profil protein menggunakan metode SDS PAGE menunjukkan sedikitnya protein rekombinan Ag38 kDa yang terekspresi dalam bentuk solubel. Proses pemurnian protein memperlihatkan protein dengan kemurnian yang lebih baik walaupun hasilnya rendah.
English Abstract
Mycobacterium tuberculosis with its 38 kDa antigen is lipoglycoprotein combined with phosphate transport protein (PSTS-1). This PSTS-1 is as initial receptor for active protein transport. 38 kDa antigen can be used to distinguish between pure infection from Mycobacterium tuberculosis with other species of Mycobacterium. Thus, 38 kDa antigen is a potential candidate as serodiagnostic agent. Biomedical Laboratory of Medical Faculty of Brawijaya University has cloned and expressed Ag38 kDa Mycobacterium tuberculosis pab gene. In previous study, Mycobacterium tuberculosis Ag38 kDa purification was conducted using IMAC (Immobilized Metal Affinity Chromatography) method with Protino kit. However, copurified protein that was found ini elution as a result of purification still involved. In this study, we purified protein recombinant Ag38 kDa using IMAC method with Thermo Hispur Ni-NTA kit. Profil protein using SDS PAGE method showed soluble protein with better level of purity although less ammount produced.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2017/424/051712048 |
| Uncontrolled Keywords: | IMAC, Mycobacterium tuberculosis, protein rekombinan Ag38 kDa,IMAC, Mycobacterium tuberculosis, recombinant protein Ag38 kDa |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics > 613.28 Specific nutritive elements > 613.282 Proteins |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 18 Jan 2018 03:12 |
| Last Modified: | 17 Oct 2020 04:45 |
| URI: | http://repository.ub.ac.id/id/eprint/8106 |
Actions (login required)
 |
View Item |