Lutfiana, Nihayatul (2017) Potensi Bacillus Thuringiensis Isolat Bareng Tenes, Malang Sebagai Agen Pengendali Larva Aedes Aegypti Dan Identifikasi Berdasarkan Sekuen 16s Rdna. Sarjana thesis, Universitas Brawijaya.
Abstract
Demam Berdarah Dengue merupakan penyakit yang disebabkan oleh virus Dengue dan nyamuk Aedes sp. betina sebagai vektornya. Jumlah kasus penyakit ini mengalami peningkatan di berbagai daerah termasuk Indonesia, salah satunya di Bareng Tenes, Malang, Jawa Timur. Penelitian ini bertujuan untuk mengidentifikasi potensi isolat B. thuringiensis dari Bareng Tenes, Malang sebagai agen pengendali larva Ae. aegypti instar ketiga dan identifikasi berdasarkan sekuen 16S rDNA. Nyamuk Ae. aegypti dipelihara di Laboratorium Ekologi dan Diversitas Hewan, sedangkan sampel dianalisis di Laboratorium Mikrobiologi Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya. Sampel air dan tanah diambil di 12 titik di area Bareng Tenes. Bakteri diisolasi menggunakan media selektif B. thuringiensis. Isolat yang mirip B. thuringiensis diuji toksisitasnya terhadap larva Ae. aegypti instar ketiga. Nilai LC50-96 jam ditentukan dengan analisis probit. Uji beda hasil penelitian menggunakan ANOVA jika terdapat perbedaan nyata dilanjutkan dengan Tukey HSD (α : 0,05). Isolat yang paling unggul diidentifikasi berdasarkan sekuen 16S rDNA. Urutan sekuen DNA yang diperoleh disejajarkan dengan bakteri acuan menggunakan program BLAST, kemudian dibuat pohon filogeni menggunakan program MEGA ver. 6.06. Berdasarkan penelitian diperoleh dua isolat yang berpotensi sebagai agen pengendali larva Ae. aegypti instar ketiga yaitu BA02b dan BA03a. Isolat BA02b merupakan isolat yang paling efektif (LC50-96 jam = 2,75 x 107 sel/mL). Sekuen 16S rDNA isolat BA02b teridentifikasi sebagai Bacillus thuringiensis var. israelensis BGSC4Q2 (similaritas sebesar 99 %).
English Abstract
Dengue is mosquito-borne viral disease that rapidly spread in all regions in recent years. Dengue virus is transmitted by female Aedes species.The number of dengue fever cases increased in many geographic regions including Indonesia and one of them occurred in Bareng Tenes, Malang, East Java. The objectives of this research were to identify the potency of B. thuringeinsis from Bareng Tenes, Malang as biological agent to control third instar of Ae. aegypti larvae and to identify the potential B. thuringiensis isolate based on 16S rDNA sequence. B. thuringiensis was isolated from water and soil which carried out from 12 sites in Bareng Tenes area. Bacterial isolation was performed using B. thuringiensis selective media. Some isolates had similar phenotypic characters with B. thuringiensis were continued to toxicity test against third instar of Ae. aegypti larvae. The value of LC50-96h determined using probit regression. The mean difference of B. thuringiensis was analyzed by ANOVA, then it was continued using Tukey HSD (α : 0,05). The most effective isolate was identified based on 16S rDNA sequence, then these local isolate aligned to reference isolate using BLAST program. Phylogeny tree was constructed using MEGA ver 6.06 program. Based on this research, BA02b and BA03a isolates were potencial agent to to control Ae. aegypti larvae. BA02b isolate was the most effective B. thuringirnsis (LC50-96h = 2,75 x 107 cell/mL). Based on 16S rDNA sequence, BA02b already identified as Bacillus thuringiensis var. israelensis BGSC4Q2 (99 % similarities).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/288/051706149 |
| Uncontrolled Keywords: | Aedes aegypti, Bacillus thuringiensis, Bareng Tenes, uji toksisitas, 16S rDNA |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.3 Prokaryotes > > 579.362 Bacillus |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 20 Oct 2017 08:27 |
| Last Modified: | 24 Nov 2021 02:02 |
| URI: | http://repository.ub.ac.id/id/eprint/4111 |
![[thumbnail of BAGIAN DEPAN.pdf]](https://repository.ub.ac.id/style/images/fileicons/text.png) |
Text
BAGIAN DEPAN.pdf Download (230kB) |
Preview |
Text
BAB V.pdf Download (85kB) | Preview |
Preview |
Text
LAMPIRAN.pdf Download (674kB) | Preview |
Preview |
Text
DAFTAR PUSTAKA.pdf Download (180kB) | Preview |
Preview |
Text
BAB I.pdf Download (237kB) | Preview |
Preview |
Text
BAB IV.pdf Download (298kB) | Preview |
Preview |
Text
BAB II.pdf Download (691kB) | Preview |
Preview |
Text
BAB III.pdf Download (397kB) | Preview |
Actions (login required)
 |
View Item |