Modulation Of The Tgf-β Signaling Pathway And Induction Of Apoptosis In Colorectal Cancer Cells By Caulerpa Racemosa Extract: An In Vitro Investigation.

Bdira, Sarra Ben and dr. Happy Kurnia Permatasari, Ph.D and dr. Hikmawan Wahyu Sulistomo, PhD (2024) Modulation Of The Tgf-β Signaling Pathway And Induction Of Apoptosis In Colorectal Cancer Cells By Caulerpa Racemosa Extract: An In Vitro Investigation. Magister thesis, Universitas Brawijaya.

English Abstract

Colorectal cancer (CRC) poses a significant global health burden, presenting significant morbidity and mortality rates worldwide. Despite advancements in treatment modalities, the need for innovative therapeutic strategies remains paramount to improve patient outcomes and combat this devastating disease. In this context, natural compounds have garnered attention for their potential anticancer properties, offering a promising avenue for novel treatment approaches. The focus of this study is to assess Caulerpa racemosa extract's, as a natural compound, possible therapeutic valuein modulating TGF-β signaling pathway and inducing apoptosis in CRC cells. HT-29 cells, widely recognized for their relevance in CRC research, were exposed to an n-hexane extract of C. racemosa. Immunofluorescence analysis was employed to evaluate TGF-β1 expression levels, providing insights into the extract's ability to modulate the TGF-β signaling pathway. Additionally, apoptosis induction was quantified using annexin V and propidium iodide staining via flow cytometry, enabling a detailed characterization of the extract's pro-apoptotic effects. The study revealed compelling findings regarding the therapeutic potential of C. racemosa extract in CRC management. Notably, the extract exhibited a significant downregulation of TGF-β1 expression compared to the control group, suggesting its potential as a CRC tumorigenesis and progression inhibitor. The extract displayed robust pro-apoptotic activity, with the 800 µg/mL dose significantly increasing early apoptotic cells compared to the 1200 µg/mL dose. Other dose comparisons showed no significant differences, indicating an optimal dosage range for early apoptosis induction at 800 µg/mL. The 1200 µg/mL dose also induced a notable increase in necrotic/late apoptotic cells compared to the control, triggering a more potent apoptotic response in advanced stages. The observed dose-dependent modulation of the TGF-β signaling pathway and induction of apoptosis by C. racemosa extract hold profound implications for CRC therapy. By targeting key molecular pathways implicated in CRC pathogenesis, such as TGF-β signaling, the extract demonstrates promising anticancer properties. Furthermore, the distinct apoptotic response elicited by varying concentrations of the extract highlights the importance of dosage optimization in maximizing therapeutic efficacy while minimizing adverse effects. The observed dose-dependent effects on early and late apoptotic cells suggest potential applications as a preventive or therapeutic agent in CRC treatment.X By targeting the TGF-β signaling pathway and inducing apoptosis in CRC cells, the extract holds promise as a valuable addition to the armamentarium against this devastating disease. To completely understand the underlying molecular pathways driving the anticancer actions of C. racemosa extract, additional research is required. Furthermore, exploring its efficacy in in vivo models and investigating potential synergistic interactions with existing treatment modalities could further enhance its clinical utility. Additionally, assessing its efficacy in vivo and exploring potential synergistic effects with existing treatment modalities could enhance its clinical utility.

Item Type:	Thesis (Magister)
Identification Number:	0424060029
Divisions:	S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran
Depositing User:	Unnamed user with username nova
Date Deposited:	10 Jul 2024 04:42
Last Modified:	10 Jul 2024 04:42
URI:	http://repository.ub.ac.id/id/eprint/221180

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