Widyani, Elsa Larissa (2020) Konjugasi Molekul Adhesi Pili Vibrio Cholerae Bm 37,8 Kda Dengan Cholerae Toxin B (CTB) Berefek Protektif Terhadap Infeksi Shigella flexneri. Magister thesis, Universitas Brawijaya.
Abstract
Diare adalah penyakit endemis di Indonesia yang memiliki angka prevalensi sebesar 8% pada Rikesdas 2018. Penyebab terbanyak diare adalah Vibrio cholerae dan Shigella flexneri sehingga dibutuhkan intervensi yang dapat menurunkan penyakit shigella dan kolera berupa vaksinasi. Vaksinasi yang telah ada hanya memberikan efek proteksi sebesar 2% pada anak usia <5 tahun terhadap kolera saja. Vaksin yang ada untuk mengatasi kolera dan shigella belum ada. Diketahui bahwa vaksin molekul adhesi Vibrio cholerae pada subunit pili dengan berat molekul 37,8 kDa yang dikonjugasikan dengan cholerae toxin subunit B (CTB) dapat menghambat proses attachment dan diare pada kolera. Molekul adhesi memiliki sifat yaitu saling bereaksi silang. Terdapat informasi bahwa terjadi reaksi silang antigenik antara molekul adhesi Vibrio cholerae dan Shigella flexneri. Sehingga dibuatlah penelitian untuk mengevaluasi apakah vaksinasi konjugasi molekul adhesi pili Vibrio cholerae BM 37,8 kDa dengan CTB dapat memberikan efek protektif terhadap infeksi Shigella flexneri. Penelitian dilakukan secara in vivo pada mencit balb/c yang diberikan vaksinasi oral dengan dosis 250μg pada hari ke-7, 14, 21, dan 28. Selanjutnya mencit dibedah dan discrapping mukosa ususnya untuk dilakukan pengukuran kadar protein β-defensin menggunakan metode ELISA. Didapatkan hasil kadar β-defensin meningkat secara signifikan pada mencit yang divaksinasi. Penelitian dilanjutkan dengan mengevaluasi progresifitas diare menggunakan metode mice ligated ileal loop (MLIL) secara ex vivo untuk selanjutnya dilakukan pengukuran massa usus. Didapatkan hasil bahwa mencit yang tidak divaksinasi namun diinfeksikan oleh Shigella flexneri atau Vibrio cholerae, terdapat peningkatan massa usus hingga menit ke-60 tanpa ada penurunan massa usus. Sedangkan mencit yang divaksinasi lalu diinfeksikan dengan Shigella flexneri atau Vibrio cholerae, massa usus meningkat namun dengan cepat kembali turun ke massa awal. Hasil ini menunjukkan adanya hambatan progresifitas diare selama infeksi Vibrio cholerae dan Shigella flexneri. Kesimpulannya, vaksinasi molekul adhesi Vibrio cholerae pada subunit pili dengan berat molekul 37,8 kDa yang dikonjugasikan dengan CTB memberikan efek protektif terhadap infeksi Vibrio cholerae dan Shigella flexneri.
English Abstract
Diarrhea is an endemic disease in Indonesia which has prevalence 8% in Rikesdas 2018. The most common causes of diarrhea is Vibrio cholerae and Shigella flexneri, which needs intervention in the form of vaccination. Existing vaccination only provides 2% protective effect against cholerae in children <5 years old. There is no vaccine available to treat both cholerae and shigellae. It is known that the Vibrio cholerae‘s adhesion molecule in pili subunit with molecular weight of 37.8 kDa conjugated with cholerae toxin subunit B (CTB) can inhibit the process of attachment and diarrhea in cholera. Adhesion molecules have the property of cross-reacting with each other. There is information that antigenic cross-reactivity occurs between the adhesion molecule of Vibrio cholerae and Shigella flexneri. So this study was conducted to evaluate whether the conjugated vaccine of Vibrio cholerae‘s adhesion molecule MW 37.8 kDa with CTB can have a protective effect against Shigella flexneri’s infection. The study was conducted in vivo on balb/c mice given vaccination orally 250 μg on day 7, 14, 21, and 28. Furthermore, mice were sacrificed and their intestinal mucosa was scrapped for measurement of β-defensin protein levels using the ELISA method. The results showed that β-defensin levels increased significantly in vaccinated mice. The study was continued by evaluating the progression of diarrhea using the mice ligated ileal loop (MLIL) method ex vivo for subsequent measurement of intestinal mass. The results showed that mice that were not vaccinated but were infected with Shigella flexneri or Vibrio cholerae, had an increase in intestinal mass up to the 60th minute without further decrease in intestinal mass. Whereas the vaccinated mice that were infected with Shigella flexneri or Vibrio cholerae, the intestinal mass increased but quickly returned to the initial mass. These results indicate the inhibition of diarrhea’s progression during infection of Vibrio cholerae and Shigella flexneri. In conclusion, vaccination of Vibrio cholerae’s adhesion molecule in pili subunit with molecular weight 37.8 kDa conjugated with CTB has a protective effect against infections of Vibrio cholerae and Shigella flexneri.
Other obstract
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| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.932/WID/k/2019/042002719 |
| Uncontrolled Keywords: | VIBRIO CHOLERA, SHIGELLA FLEXNERI |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.93 Clostridium infections, diphtheria, cholera, dysenteries, protozoan infections > 616.932 Cholera |
| Divisions: | S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 05 Mar 2020 03:34 |
| Last Modified: | 25 Sep 2024 02:13 |
| URI: | http://repository.ub.ac.id/id/eprint/179947 |
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