Puspitasari, Asthervina Widyastami (2017) Studies On The Effect Of Cinnamaldehyde On Innate Immune Response Against Nervous Necrosis Virus In Medaka Olhe-13. Magister thesis, Universitas Brawijaya.
Abstract
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English Abstract
Nervous necrosis virus (NNV) is a bipartite single-stranded positivesense RNA molecule virus, which caused severe nervous necrosis disease in many kinds of grouper species. In the present study medaka liver cell line OLHE-131 and medaka fish were used as models to study the effect of cinnamaldehyde on the antiviral activity against RGNNV infection. Cytotoxicity assay showed that 5 µg/mL and 10 g/mL were the appropriate concentration for treating OLHE-131, and the mRNA expressions level of immune-related genes such as IFN-α, IFN-d, MX, ISG15, ISG56, GIG1-α were increased in the presence of 5 µg/mL and 10 g/mL of cinnamaldehyde. Based on the morphology assay, 5 µg/mL of cinnamaldehyde can prevent NNVinduced CPE suggesting cinnamaldehyde potential exhibit antiviral activity against NNV infection. Dietary supplementation with cinnamaldehyde (60 mg/kg) in adult medaka for one month revealed the immune-related genes suchiii as IFN-α, IFN-d, MX, ISG15, ISG56, GIG1-α were significantly increased suggesting cinnamaldehyde possess immunomodulatory functions and potential is an effective immunostimulatory against NNV virus in aquaculture
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/547.036/PUS/s/2017/041802706 |
| Uncontrolled Keywords: | ALDEHYDES, NECROSIS, DISEASES - ANIMAL MODELS, FISH AS LABORATORY ANIMALS |
| Subjects: | 500 Natural sciences and mathematics > 547 Organic chemistry > 547.03 Oxy and hydroxy compounds > 547.036 Aldehydes and ketones |
| Divisions: | S2/S3 > Doktor Ilmu Perikanan dan Kelautan, Fakultas Perikanan dan Ilmu Kelautan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 21 Dec 2018 01:35 |
| Last Modified: | 22 Oct 2021 03:39 |
| URI: | http://repository.ub.ac.id/id/eprint/162248 |
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