Beberapa Parameter Motilitas Spermatozoa Semen Cair Pada Tiga Bangsa Sapi Lokal Dengan Tiga Pengencer Berbeda Selama Simpan Dingin

Ratnawati, Dian (2017) Beberapa Parameter Motilitas Spermatozoa Semen Cair Pada Tiga Bangsa Sapi Lokal Dengan Tiga Pengencer Berbeda Selama Simpan Dingin. Magister thesis, Universitas Brawijaya.

Abstract

Motilitas spermatozoa merupakan salah satu parameter yang menentukan kualitas semen sapi jantan. Tujuan dari penelitian ini adalah: (1). Mengkaji berbagai parameter motilitas spermatozoa selama penyimpanan 3-5°C dengan menggunakan pengencer berbeda pada bangsa sapi lokal yang sama. (2). Mengkaji berbagai parameter motilitas spermatozoa selama penyimpanan 3-5°C pada bangsa sapi lokal yang berbeda dengan pengencer yang sama. Penelitian ini dilaksanakan di kandang Loka Penelitian Sapi Potong selama 3 bulan. Materi yang digunakan sapi jantan PO, Bali dan Madura masing-masing 2 ekor. Penampungan semen segar sapi jantan menggunakan vagina buatan sampai dengan 10 ulangan. Dilakukan analisa semen, semen dengan motilitas progresif >70% diproses menjadi semen cair. Terdapat 3 pengencer yang digunakan yaitu tris aminomethane, CEP-2 dan susu skim. Konsentrasi semen cair dibuat sampai dengan 100 juta/ml. Semen cair disimpan selama 5 hari dalam refirgerator suhu 3-5°C dan dilakukan observasi motilitas semen cair harian (H0-H5) dengan menggunakan SCA. Parameter yang diukur pada semen segar (volume, pH, warna, konsistensi, konsentrasi, gerak massa, viabilitas, motilitas, total spermatozoa motil, abnormalitas spermatozoa) dan semen cair (motilitas, motilitas progresif, spermatozoa hiperaktif, VCL, VSL, VAP, LIN, STR, WOB , BCF dan ALH). Rancangan percobaan menggunakan RAK tersarang. Analisis data menggunakan Minitab 17. Parameter motilitas yang dapat dipertahankan dengan baik sampai dengan hari ke 5 pada sapi PO adalah: motilitas progresif (62,1 ± 6,5 %), VSL (21,3 ± 3,7 μm/s), VAP (25,6 ± 3,1 μm/s), LIN (52,0 ± 10,7 %), STR (82,7 ± 6,6 %), WOB (62,2 ± 8,4 %), ALH (1,8 ± 0,3 μm) dan BCF (12,3 ± 1,0 Hz). Parameter motilitas yang dapat dipertahankan dengan baik sampai dengan hari ke 5 pada sapi Bali adalah: motilitas progresif (61,4 ± 10,6 %), hiperaktif spermatozoa (8,4 ± 3,9 %), VSL (20,6± 3,2 μm/s), VAP (25,7 ± 4,0 μm/s), LIN (49,1 ± 3,2 %), WOB (61,2 ± 3,4 %), ALH (2,1 ± 0,2 μm) dan BCF (11,4 ± 1,2 Hz). Parameter motilitas yang dapat dipertahankan dengan baik sampai dengan hari ke 5 pada sapi Madura adalah: motilitas (75,3 ± 12,2 %), motilitas progresif (51,1 ± 10,4 %), LIN (51,4 ± 6,0 %), STR (78,7 ± 2,6 %), WOB (65,2 ± 6,8 %), ALH (1,7 ± 0,1 μm) dan BCF (14,1 ± 3,5 Hz). Pengencer terbaik dalam menjaga motilitas spermatozoa semen cair selama simpan dingin adalah CEP-2. Pengencer CEP-2 pada sapi PO, Bali dan Madura dapat mempertahankan motilitas progresif tetap tinggi (62,1 ± 6,5 %; 61,4 ± 10,6 %; 51,1 ± 10,4 %) dan hiperaktif spermatozoa tetap rendah 8,4 ± 3,9 % (sapi Bali). Bangsa sapi Madura mempunyai motilitas spermatozoa semen cair terbaik selama simpan dingin, mempunyai nilai hiperaktif spermatozoa yang rendah yaitu 5,1 ± 2,3 (tris aminomethane); 2,0 ± 1,1 %( CEP-2) serta nilai VSL dan VAP tetap tinggi yaitu 22,2 ± 4,4 μm/s; 27,8 ± 6,3 μm/s (susu skim).

English Abstract

Sperms motility is one of parameters which is determine semen quality of bull. The purpose of this study are: (1). Assess sperm motility parameters during 3-5 ° C storage by using different diluents in the same local bull. (2). Assess sperm motility parameters during 3-5 ° C storage in different local bulls with the same diluent. This research was conducted at Beef Cattle Research Institute stall for 3 months. The material used by PO, Bali and Madura bulls (2 heads respectively). Collecting fresh semen by using artificial vagina up to 10 replications. Fresh semen quality with motility >70% and continue to be prosessed into liquid semen. There are 3 diluents used i.e. tris aminomethane, CEP-2 and skim milk. The concentration of liquid semen was made up to 100 million/ml. The liquid semen was stored for 5 days in refrigerator and observed daily motility of liquid semen (H0-H5) by using SCA. Parameters measured on fresh semen (volume, pH, color, consistency, concentration, mass motion, viability, motility, totally motile sperms, sperm abnormalities) and liquid semen (motility, progressive motility, hyperactive sperms, VCL, VSL, VAP, LIN , STR, WOB, BCF and ALH). Experimental design using nested RAK. Data analysis using Minitab 17. The best motility parameters maintained up to 5 days in PO bull were: progressive motility (62,1 ± 6,5%), VSL (21,3 ± 3,7 μm / s), VAP (25,6 ± 3,1 μm / s), LIN (52,0 ± 10,7%), STR (82,7 ± 6,6%), WOB (62,2 ± 8,4%), ALH (1,8 ± 0,3 μm) and BCF (12,3 ± 1,0 Hz). The best motility parameters maintained up to 5 days in Bali bull were: progressive motility (61,4 ± 10,6%), sperms hyperactive (8,4 ± 3,9%), VSL (20,6 ± 3,2 μm / s), VAP (25,7 ± 4,0 μm / s), LIN (49,1 ± 3,2%), WOB (61,2 ± 3,4%), ALH (2,1 ± 0,2 μm) and BCF (11,4 ± 1,2 Hz). The best motility parameters maintained up to 5 days of Madura bull were: motility (75,3 ± 12,2%), progressive motility (51,1 ± 10,4%), LIN (51,4 ± 6,0%), STR (78,7 ± 2,6%), WOB (65,2 ± 6,8%), ALH (1,7 ± 0,1 μm) and BCF (14,1 ± 3,5 Hz). The best diluent which can maintain sperms motility of liquid semen at cool storage is CEP-2. Diluent of CEP-2 on PO, Bali, Madura bull can maintain high progressive motility (62,1 ± 6,5 %; 61,4 ± 10,6 %; 51,1 ± 10,4 %) and low sperms hyperactive 8,4 ± 3,9 % (Bali bull). Madura bull have the best sperms motility at cool storage, which have a low sperms hyperactive and high of VSL (22,2 ± 4,4 μm/s), VAP (27,8 ± 6,3 μm/s) in skim milk.

Item Type: Thesis (Magister)
Identification Number: TES/636.083 4/RAT/b/2017/041705847
Uncontrolled Keywords: SEMEN, CATTLE - BREEDING, DISTUTION, FROZEN SEMEN
Subjects: 600 Technology (Applied sciences) > 636 Animal husbandry > 636.2 Cattle and related animals > 636.23 German, Dutch, Danish, Swiss breeds of cattle > 636.234 Dairy breeds
Divisions: S2/S3 > Magister Ilmu Ternak, Fakultas Peternakan
Depositing User: Nur Cholis
Date Deposited: 02 Aug 2017 07:10
Last Modified: 31 Dec 2020 02:45
URI: http://repository.ub.ac.id/id/eprint/963
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