Nisa, Masayu Nessya Khoirun (2017) Regenerasi Kedelai (Glycine Max L. Merr) Varietas Grobogan, Wilis Dan Dering 1 Hasil Iradiasi Sinar Gamma. Sarjana thesis, Universitas Brawijaya.
Abstract
Produksi kedelai masih belum mencukupi kebutuhan masyarakat di Indonesia. Salah satu upaya meningkatkan produksi kedelai adalah dengan penggunaan varietas unggul dan ekstensifikasi pada lahan kering. Pemuliaan mutasi in vitro merupakan salah satu cara mendapatkan varietas unggul melalui perbaikan sifat yang diinginkan. Penelitian bertujuan untuk mengetahui media terbaik guna meregenerasi planlet kedelai hasil iradiasi sinar Gamma 4. Penelitian dilaksanakan pada bulan Februari hingga Agustus 2017 di laboratorium biologi sel dan jaringan dan rumah kaca Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Bogor. Penelitian menggunakan Rancangan Acak Lengkap dengan perlakuan iradiasi sinar gamma 4 Gray. Dari hasil analisis data kombinasi media MS+BA 0,5 mg/l+Kin 0,1 mg/l merupakan media regenerasi terbaik. Terdapat pengaruh dari perlakuan iradiasi sinar Gamma 4 Gray terhadap penurunan pertumbuhan kedelai varietas Grobogan, Wilis dan Dering 1. Serta didapatkan 14 planlet kedelai yang berhasil diaklimatisasi.
English Abstract
Soybean production is still insufficient for the needs of the people in Indonesia. One of the efforts to increase soybean production is by using superior varieties. Breeding method by in vitro mutations is one way of obtaining superior varieties by improving the desired properties. The aim of this research is to know the best media to regeneratethe soybean plantation from Gamma irradiation. The study was conducted from February to August 2017 at theCell Biology LaboratoryandGreenhouse of Indonesian Biotechnology and Genetic Resources Research and Development Center, Bogor.The study used a Completely Randomized Design with 4 Gray Gamma irradiation treatment. The result of the experiment showed tha combination media MS+BA 0.5 mg/l+Kin 0.1 mg/l was the best regenerationmedia. Theeffect of Gamma 4 Gray irradiation treatment decreased of soybean growth of varieties Grobogan, Wilis and Dering 1. There were 14 soybean plantlets that acclimatized successfully.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2017/1037/051800731 |
| Uncontrolled Keywords: | Iradiasi, Mutasi, Sinar Gamma,Tanaman Kedelai, Gamma Ray, Irradiation, Mutation, Soybean. |
| Subjects: | 600 Technology (Applied sciences) > 633 Field and plantation crops > 633.3 Legumes, forage crops other than grasses and legumes > 633.34 Soybeans > 633.342 3 Soybean (Development of new varieties) > 633.342 33 Soybean (Agricultural genetics) |
| Divisions: | Fakultas Pertanian > Budidaya Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 30 Jan 2018 03:38 |
| Last Modified: | 02 Oct 2020 04:31 |
| URI: | http://repository.ub.ac.id/id/eprint/8585 |
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