Damayanti, Rosalina (2017) Pengaruh Pemberian Vaksin Outer Membrane Protein (Omp) Porphyromonas Gingivalis Terhadap Kadar Angiotensin Ii Tikus Model Hipertensi. Sarjana thesis, Universitas Brawijaya.
Abstract
Hipertensi merupakan kondisi peningkatan tekanan darah di atas level normal. Salah satu penyebabnya adalah keterlibatan dari angiotensin II, efektor utama dari Renin Angiotensin Aldosterone System (RAAS). Outer Membrane Protein (OMP) Porphyromonas gingivalis memiliki arg-gingipain (Rgp) yang diketahui memiliki mimikri molekuler dengan Malondialdehyde-Modified Low-Density Lipoprotein (MDA-LDL). Penelitian ini bertujuan untuk menganalisis pengaruh pemberian vaksin OMP P. gingivalis terhadap kadar angiotensin II tikus model hipertensi yang diinduksi DOCA-salt. Penelitian ini menggunakan true experimental design secara in vivo di laboratorium menggunakan metode randomized post test only controlled group design. Hewan coba 30 tikus wistar jantan dibagi menjadi 5 kelompok yaitu KN (tikus normal), KP (DOCA), P1 (DOCA+OMP 100μl), P2 (DOCA+OMP 100μl+Alum 100μl), dan P3 (DOCA+Alum 100μl). Perlakuan diawali dengan pemberian OMP P.gingivalis secara intraperitoneal pada 3 kelompok tikus dengan komposisi yang telah ditentukan. Setelah 10 hari pertama dari waktu pemberian OMP, dilakukan pemberian DOCA secara subkutan dengan dosis 10 mg/kgBB sebanyak 2 kali dalam seminggu selama 6 minggu yang diikuti oleh penambahan NaCl 1% pada air minum seluruh kelompok tikus (kecuali tikus KN). Pemberian OMP sebagai vaksin ulangan (booster) diberikan sebanyak 4 kali dengan jarak 10 hari dari waktu pemberian dosis vaksin sebelumnya. Setelah pemberian vaksin dan DOCA-salt, dilakukan pengukuran tekanan darah darah tikus dan dilanjutkan dengan pengukuran kadar angiotensin II. Hasil analisis statistik dengan uji one way ANOVA diperoleh nilai signifikansi sebesar 0,644 (p>0,05). Dari hasil penelitian ini dapat disimpulkan pemberian vaksin OMP P. gingivalis tidak berpengaruh terhadap kadar angiotensin II tikus model hipertensi yang diinduksi DOCA-salt.
English Abstract
Hypertension is a condition of high blood pressure above normal levels. One of the causes is due to of Angiotensin II, the main effector of Renin Angiotensin Aldosterone System (RAAS). Outer Membrane Protein (OMP) of Porphyromonas gingivalis has arg-gingipain (Rgp) that is known has molecular mimicry with Malondialdehyde-Modified Low-Density Lipoprotein (MDA-LDL). This study aims to analyze the influence of P. gingivalis OMP vaccine on hypertension rat model’s angiotensin II level that induced by DOCA-salt. This study pure experimental research in the laboratory using in vivo methods with post test only randomized controlled group design. Animals, 30 wistar rats male divided into 5 groups: KN (normal), KP (DOCA), P1 (DOCA+OMP 100 μl), P2 (DOCA+OMP 100 μl+Alum 100 μl), and P3 (DOCA+Alum 100 μl). Treatment was initiated by administering OMP and Alum intraperitoneally in accordance with the group of rats and compositions. After first 10 days administration of OMP and Alum, DOCA was given subcutaneously with dose 10 mg/kgBW twice a week for 6 weeks followed by NaCl 1% addition in drinking water throughout the mouse group (excluding KN). Administration of OMP was repeated 4 times in every 10 days intraperitoneally after previous administration. After all treatment is completed, the blood pressure and angiotensin II level will be measured. The results of the analysis with the one-way ANOVA test was obtained significantly by 0,644 (p>0,05). So, it can be concluded that the administration of P. gingivalis OMP vaccine does not affect on hypertension rat model’s angiotensin II level that induced by DOCA-salt.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2017/506/051800707 |
| Uncontrolled Keywords: | Angiotensin II, Renin Angiotensin Aldosterone System (RAAS), Outer Membrane Protein (OMP), Porphyromonas gingivalis (P.gingivalis), Deoxycorticosterone Acetat (DOCA), Angiotensin II, Renin Angiotensin Aldosterone System (RAAS), Outer Membrane Protein (OMP), Porphyromonas gingivalis (P.gingivalis), Deoxycorticosterone Acetat (DOCA) |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.37 Immunologic drugs and immune serums > 615.372 Vaccines |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 23 Jan 2018 03:52 |
| Last Modified: | 25 Oct 2021 03:37 |
| URI: | http://repository.ub.ac.id/id/eprint/8326 |
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