Rosandra, Almira Sophia Ega (2017) Pengaruh Induksi DMBA (7,12-dimethylbenz (α) anthracene) Terhadap kadar MDA (Malondialdehyde) Pada Serum dan Ekspresi IL-1β (Interleukin-1 beta) Pada Hepar Tikus (Rattus norvegicus) Model Kanker Mammae. Sarjana thesis, Universitas Brawijaya.
Abstract
Kanker mammae merupakan ketidaknormalan sel pada duktus dan jaringan mammae serta menginvasi jaringan disekitar mammae. Senyawa DMBA (Dymethylbenz(α) Anthracene) diketahui merupakan senyawa karsinogenik yang mampu menyebabkan terbentuknya kanker. Induksi DMBA secara subkutan pada mammae menghasilkan produk samping radikal bebas atau ROS. Radikal bebas mampu menyebabkan stress oksidatif yang menyerang organ salah satunya organ hepar. Stress oksidatif sistemik bereaksi dengan lipid menyebabkan terjadinya peroksidasi lipid yang menghasilkan produk akhir berupa malondialdehyde (MDA) dan pada organ hepar memicu menginduksi sitokin proinflamatori salah satunya adalah IL-1β. Penelitian ini bertujuan untuk mengetahui pengaruh DMBA terhadap perubahan kadar Malondialdehyde (MDA) serum dan ekspresi IL-1β di organ hepar. Penelitian ini dilakukan dengan menggunakan hewan coba tikus putih (Rattus norvegicus) strain Sprague dawley betina umur 10-12 minggu dengan berat rata-rata 150-200 gram. Hewan tikus dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol negatif. Induksi DMBA diberikan Multiple Low Dose (MLD) sebesar 10 mg/kg BB secara subkutan pada mammae setiap dua hari sekali sebanyak 10x induksi. Induksi estrogen diberikan dosis 20.000 IU/kg BB secara intramuskular setiap 4 hari sekali sebanyak 5 kali. Parameter yang diamati adalah MDA pada serum dan ekspresi IL-1β pada organ hepar. Kadar MDA dalam serum diamati dengan menggunakan uji Thiobarbituric Acid (TBA) dan ekspresi IL-1β diamati dengan metode imunohistokimia. Analisa data dilakukan secara kuantitatif dengan uji T tidak berpasangan. Hasil penelitian menunjukkan bahwa induksi DMBA memberikan pengaruh signifikan terhadap peningkatan kadar MDA serum sebesar 14x (7,27±1,90) dan ekspresi IL-1β sebesar 19x (0,217±0,028) pada hepar tikus dengan α=0,05. Kesimpulan penelitian ini adalah induksi DMBA dapat meningkatkan kadar MDA serum dan ekspresi IL-1β pada hepar tikus.
English Abstract
Mammary cancer is an abnormal cell in the ductus and mammary tissues and invades the tissues around the mammary. DMBA compounds (Dymethylbenz (α) Anthracene) is carcinogenic compounds capable of causing to formation of cancer. Subcutaneous DMBA induction in mammary produce free radicals or ROS (Reactive Oxygen Species). Free radicals are capable of causing oxidative stress that attacks the organs such as liver. Systemic oxidative stress reacts with lipids to cause lipid peroxidation resulting in malondialdehyde (MDA) and triggering proinflammatory cytokines IL-1β in liver. This study was aimed to determine the effect of DMBA induction on serum MDA levels and IL-1β expression in liver. Animal studies of the model of mammary cancer were performed using a white rats (Rattus norvegicus) strain Sprague dawley female aged 10-12 weeks with an average weight of 150-200 grams. The rats were divided into 2 groups: the treatment group and the control group. Induction of DMBA by 10 mg/kg BW of subcutaneous mammary on the mammary every two days at 10x induction. Induction of estrogen dose 20,000 IU/kg intramuscularly every 4 days for 5 times. The parameters observed were serum MDA and IL-1β expression in liver. Serum MDA levels were observed using the Thiobarbituric Acid (TBA) test and IL-1β expression was observed by immunohistochemical methods. The data were analyzed quantitatively using an unpaired T test. The results showed that the induction of DMBA affected significantly to increase MDA levels by 14x (7.27 ± 1.90) in serum and expression of IL-1β by 19x (0.217 ± 0.028) in rat liver with α = 0,05. The conclusion of this study was induction of DMBA can increase the levels of serum MDA and IL-1β expression in rats liver.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2017/206/051800737 |
| Uncontrolled Keywords: | Kanker Mammae, DMBA, MDA, IL-1β, Serum, Hepar, Mammary Cancer, DMBA, MDA, IL-1β, Serum, Hepar |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.99 Tumors and miscellaneous communicable diseases > 616.994 Cancers > 616.994 49 Breast cancer |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 23 Jan 2018 01:19 |
| Last Modified: | 26 Oct 2021 03:07 |
| URI: | http://repository.ub.ac.id/id/eprint/8302 |
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