Isolasi Dan Identifikasi Bakteri Endofit Mangrove Sonneratia Alba Penghasil Enzim L-Asparaginase

Lestari, Puspita Dwi (2017) Isolasi Dan Identifikasi Bakteri Endofit Mangrove Sonneratia Alba Penghasil Enzim L-Asparaginase. Sarjana thesis, Universitas Brawijaya.

Abstract

Enzim L-asparaginase (L-asparaginase aminohidrolase; E.C 3.5.1.1) merupakan enzim yang mengkatalisis hidrolisis gugus amida dari L-asparagin menjadi L-aspartat dan amonia. Aplikasi dalam dunia medis L-asparaginase adalah sebagai agen kemoterapi pengobatan kanker dan dalam pangan digunakan untuk mencegah terbentuknya akrilamid L-asparaginase dapat ditemukan pada jaringan hewan, mikroba, dan tumbuhan. Salah satu tumbuhan yang berpotensi untuk menghasilkan enzim L-asparaginase adalah endofit mangrove Sonneratia alba.Tujuan dari penelitian ini adalah menemukan bakteri penghasil enzim L-asparaginase dari endofit mangrove Sonneratia alba. Metode penelitian yang digunakan adalah deskriptif eksploratif. Pada penelitian ini sampel mangrove Sonneratia alba diambil dari Pantai Bajulmati, Malang. Bagian mangrove yang digunakan adalah akar, batang, daun dan buah, kemudian diisolasi pada media LB agar dan diskrining dengan menggunakan media selektif M-9 L-asparaginase dan didapatkan hasil bakteri penghasil L-asparaginase. Bakteri yang memiliki aktivitas enzim paling besar akan dilakukan identifikasi dengan pewarnaan Gram dan uji microbact system. Dari 8 isolat, yang menghasilkan enzim L-asparaginase adalah DSA2, DSA4, DSA5 dan BSA4. Aktivitas produksi enzim paling baik adalah DSA4 adalah bakteri Gram positif yang memiliki bentuk sel basil, menghasilkan spora, berdiameter koloni 3,82 mm, dan menurut uji microbact system yang dilakukan bakteri tersebut adalah Bacillus megaterium.

English Abstract

The enzyme L-asparaginase (L-asparaginase aminohidrolase; E.C 3.5.1.1) is an enzyme that catalyze of amide groups from L-asparagin to Laspartate and ammonia.Application L-asparaginase enzyme is have been used in clinical world for cancer therapy and food industry was used to prevent the formation of acrylamide. L-asparaginase is found in animal tissues, microbes, and plants. One of the plants that have the potential to produce L-asparaginase enzyme is mangrove endophyte Sonneratia alba. The purpose of this study was to find L-asparaginase enzyme-producing bacteria from endophytic mangrove Sonneratia alba. The research used descriptive explorative method.The samples Sonneratia alba mangroves taken from Bajulmati Beach, Malang. Part of the mangroves which used in this research are the root, stems, leaves and fruit, then samples were planted in LB Agar and screened using selective media M-9 for L-asparaginase and obtained L-asparaginase bacteria. Bacteria that had the greatest enzyme activity will be identified with Gram staining and microbact system test. From 8 isolates, bacteria which produce L-asparaginase were DSA2, DSA4, DSA5 and BSA4. The best enzyme L-asparaginase activity was DSA4, Gram-positive,basil, producing spore, 3.82 mm colony diameter, and according to microbact system test the bacteriawas Bacillus megaterium.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FPR/2017/853/051711405
Uncontrolled Keywords: L-asparaginase, bakteri endofit, Sonneratia alba, Microbact,Bacillus megaterium, L-asparaginase, endophytes bacteria, Sonneratia alba, microbact, Bacillus megaterium
Subjects: 500 Natural sciences and mathematics > 572 Biochemistry > 572.7 Enzymes
Divisions: Fakultas Perikanan dan Ilmu Kelautan > Manajemen Sumberdaya Perairan
Depositing User: Nur Cholis
Date Deposited: 20 Dec 2017 02:23
Last Modified: 26 Nov 2020 13:21
URI: http://repository.ub.ac.id/id/eprint/7263
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (71kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (26kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (7kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (2kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (82kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (30kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (8kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (2kB)

Actions (login required)

View Item View Item