Pengaruh Terapi Ekstrak Kulit Manggis (Garcinia mangostana L.) Terhadap Profil Protein Plasma Darah dan Ekspresi Interferon Gamma (IFN-γ) pada Otak Tikus (Rattus novergicus) Model Traumatic Brain Injury

Damayanti, Vanila (2017) Pengaruh Terapi Ekstrak Kulit Manggis (Garcinia mangostana L.) Terhadap Profil Protein Plasma Darah dan Ekspresi Interferon Gamma (IFN-γ) pada Otak Tikus (Rattus novergicus) Model Traumatic Brain Injury. Sarjana thesis, Universitas Brawijaya.

Abstract

Traumatic Brain Injury (TBI) merupakan suatu gangguan atau kerusakan pada kepala yang disebabkan oleh benturan fisik dari luar yang menyebabkan peningkatan tekanan intrakarnial dan timbulnya radikal bebas. Hal ini dapat mengurangi atau mengubah kesadaran yang dapat mengakibatkan rusaknya kemampuan kognitif dan fungsi fisik tubuh. Ekstrak kulit manggis (Garcinia mangostana L.) diketahui memiliki aktivitas farmakologis sebagai anti oksidan yaitu pada senyawa xanthon dan berfungsi sebagai anti apoptosis. Penelitian ini dilakukan untuk mengetahui profil protein dan ekspresi IFN-γ pada tikus putih (Rattus norvegicus) model TBI setelah diterapi dengan ekstrak kulit manggis. Penelitian dilakukan dengan menyusun 4 kelompok perlakuan yakni kelompok kontrol negatif (tikus sehat), kelompok kontrol positif model TBI, kelompok kontrol model TBI yang diterapi ekstrak kulit manggis dan kelompok kontrol model TBI yang diterapi Minocycline. Ekstrak kulit manggis diberikan dengan dosis 0,5 mL/hari selama 5 hari. Profil protein dianalisa menggunakan metode elektroforesis SDS-PAGE dan Ekspresi IFN-γ dianalisa dengan metode Immunohistokimia. Hasil penelitian menunjukkan bahwa terapi ekstrak kulit manggis mampu memperbaiki kerusakan dengan terbentuknya protein pada berat molekul 86,322 kDa dan menurunkan ekspresi IFN-γ pada jaringan otak tikus sebesar 14%. Hasil analisa statistik ekspresi menunjukkan bahwa terdapat perbedaan yang signifikan antar kelompok perlakuan (p<0,01). Kesimpulan dari penelitian ini adalah ekstrak kulit manggis dapat digunakan sebagai alternatif untuk terapi pada cedera otak.

English Abstract

Traumatic Brain Injury (TBI) is a disorder or damage to the head caused by a physical impact from the outside which causes increased intracarnial pressure and the onset of free radicals. This can reduce or alter the awareness that can cause damage to cognitive abilities and physical functioning of the body. Mangosteen extract (Garcinia mangostana L.) is known to have pharmacological activity as an antioxidant that is in xanthon compounds and serves as anti apoptosis. This study was conducted to determine the protein profile and IFN-γ expression in white rats (Rattus norvegicus) model of TBI after treatment with mangosteen extract. This study used the experimental animals grouped into 4 groups treatment: negative group, positive group(TBI), TBI group with mangosteen pericarp extract therapy dose 0,5 mL/day for 5 days and TBI group with minocycline therapy dose 0,5 mL/day for 5 days. Protein profiles were analyzed using SDS-PAGE electrophoresis method and IFN-γ expression was analyzed by Immunohistochemical method. The results showed that mangosteen extract therapy was able to repair the damage by forming new proteins 86,322 kDa and decreasing IFN-γ expression up to 14%. The result of statistical analysis of protein profile and SOD expression showed that there were significantly differences between treatment groups (p<0,01). The conclusion of this study is the extract of mangosteen can be used as an alternative to therapy on brain injury.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FMIPA/2017/307/051709647
Uncontrolled Keywords: Traumatic brain injury, ekstrak kulit manggis, profil protein, IFN-γ
Subjects: 500 Natural sciences and mathematics > 543 Analytical chemistry > 543.599 3 Analytical chemistry (Mammals-Mouse)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Nur Cholis
Date Deposited: 20 Oct 2017 01:40
Last Modified: 23 Nov 2021 07:41
URI: http://repository.ub.ac.id/id/eprint/4064
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (70kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (28kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (7kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (2kB)
[thumbnail of BAGIAN DEPAN.pdf] Text
BAGIAN DEPAN.pdf

Download (354kB)
[thumbnail of BAB V.pdf] Text
BAB V.pdf

Download (181kB)
[thumbnail of LAMPIRAN.pdf] Text
LAMPIRAN.pdf

Download (1MB)
[thumbnail of BAB IV.pdf] Text
BAB IV.pdf

Download (605kB)
[thumbnail of DAFTAR PUSTAKA.pdf] Text
DAFTAR PUSTAKA.pdf

Download (204kB)
[thumbnail of BAB II.pdf] Text
BAB II.pdf

Download (425kB)
[thumbnail of BAB III.pdf] Text
BAB III.pdf

Download (339kB)
[thumbnail of BAB I.pdf] Text
BAB I.pdf

Download (208kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (39kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (5kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (37kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (5kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (38kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (5kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (18kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (7kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (3kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (38kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (5kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (35kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (5kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (16kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (7kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (19kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (7kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (5kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)

Actions (login required)

View Item View Item