Pengaruh Terapi Ekstrak Kulit Manggis (Garcinia mangostana L.) terhadap Aktivitas Protease Serum Darah dan Ekspresi Malondialdehida (MDA) pada Otak Tikus (Rattus norvegicus) Model Traumatic Brain Injury

Ratnasari, Nur Indah (2017) Pengaruh Terapi Ekstrak Kulit Manggis (Garcinia mangostana L.) terhadap Aktivitas Protease Serum Darah dan Ekspresi Malondialdehida (MDA) pada Otak Tikus (Rattus norvegicus) Model Traumatic Brain Injury. Sarjana thesis, Universitas Brawijaya.

Abstract

Cedera otak traumatis merupakan penyebab gangguan fungsi kognitif dan fisik yang sifatnya menetap atau sementara dan disertai hilangnya atau berubahnya tingkat kesadaran dan menimbulkan kerusakan maupun kematian sel pada otak. Kematian sel atau apoptosis ini dapat menyebabkan timbulnya inflamasi maupun stres oksidatif dan menghasilkan reactive oxygen species (ROS). Penggunaan ekstrak kulit manggis berfungsi untuk mencegah terjadi timbulnya inflamasi dan stres oksidatif. Penelitian ini bertujuan untuk mengetahui peranan terapi ekstrak kulit manggis pada ekspresi organ otak dan aktivitas protease pada serum darah tikus. Selain itu digunakan minocycline sebagai standart uji dan perbandingan dari ekstrak kulit manggis. Hasil penelitian ini membentuk 4 kelompok perlakuan yaitu kelompok negatif, kelompok positif model TBI, kelompok TBI dengan terapi ekstrak kulit manggis dosis 0,5 mL/hari selama 5 hari dan kelompok TBI dengan terapi minocycline dosis 0,5 mL/hari selama 5 hari. Hasil penelitian menunjukkan bahwa terapi ekstrak kulit manggis pasca cedera otak dapat menurunkan ekspresi malondialdehida (MDA) sebesar 32,29% dan aktivitas protease dalam serum darah tikus sebesar 47,62%. Hasil analisa statistik ekspesi MDA dan aktivitas protease menunjukkan terdapat perbedaan yang sangat nyata antar kelompok perlakuan (p<0,01). Penelitian ini dapat disimpulkan bahwa terapi ekstrak kulit manggis dapat digunakan saat terjadinya cedera otak traumatis.

English Abstract

Traumatic brain injury is a cause of persistent or temporary cognitive and physical dysfunction and accompanied by loss or altered levels of consciousness and cause damage and cell death in the brain. This cell death or apoptosis can cause both inflammation and oxidative stress and produce reactive oxygen species (ROS). The use of mangosteen skin extract serves to prevent the occurrence of inflammation and oxidative stress. This study aims to determine the role of mangosteen skin extract therapy on brain organ expression and protease activity in rat blood serum. Also used minocycline as a standard test and a comparison of mangosteen skin extract. The results of this study formed 4 groups of treatment that is negative group, positive group of TBI model, TBI group with mangosteen skin extract therapy dose 0.5 mL / day for 5 days and TBI group with minocycline therapy dose 0.5 mL / day for 5 days. The results showed that mangosteen skin extract therapy after brain injury can decrease expression of malondialdehyde (MDA) of 32,29% and protease activity in rat blood serum equal to 47,62%. Results of statistical analysis of MDA expression and protease activity showed a very significant difference between treatment groups (p <0.01). This study can be concluded that mangosteen skin extract therapy can be used when the occurrence of traumatic brain injury

Item Type: Thesis (Sarjana)
Identification Number: SKR/FMIPA/2017/306/051709646
Uncontrolled Keywords: Garcinia mangostana L., malondialdehida (MDA), aktivitas protease, inflamasi, stres oksidatif, traumatic brain injury
Subjects: 500 Natural sciences and mathematics > 543 Analytical chemistry > 543.599 3 Analytical chemistry (Mammals-Mouse)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Nur Cholis
Date Deposited: 20 Oct 2017 01:31
Last Modified: 23 Nov 2021 07:43
URI: http://repository.ub.ac.id/id/eprint/4062
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