Zafirah, Yasmin (2017) Studi Reaksi Hidrolisis Enzimatis terhadap Senyawa l-Mentil Asetat dengan Enzim Lipase dari Candida antarctica Recombined Aspergillus oryzae secara In Vitro dan In Silico. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian mengenai hidrolisis l-mentil asetat terkatalisis enzim lipase dari Candida antarctica Recombined Aspergillus oryzae telah diamati secara in vitro dan in silico. Reaksi secara in vitro dilakukan dengan variasi pelarut dan waktu. Hasil yang diperoleh yaitu enzim lipase dari Candida antarctica recombined Aspergillus oryzae dapat mengkatalisis reaksi hidrolisis l-mentil asetat menghasilkan l-mentol dengan hasil terbaik pada jam ke-16 dengan pelarut etanol dengan % konversi sebesar 13,76%. Setelah dilakukan uji sceara in silico dengan metode docking, telah diamati mekanisme antara residu asam amino terhadap makromlekul. Serta pengaruh pelarut terhadap interaksi tersebut. Residu yang terlibat adalah Glu, Thr, Asp, Gln, Ser, His. Pelarut etanol menunjukkan interaksi yang baik dengan makromolekul dengan membentuk ikatan hidrogen.
English Abstract
Research about the hydrolysis of l-mentil acetate catalyzed by lipase enzyme from Candida antarctica recombined Aspergillus oryzae has been observed by in vitro and in silico. The reaction by in vitro method performed with variations of solvent and time. The result obtained that lipase enzyme from Candida antarctica recombined Aspergillus oryzae is being able to catalyze the hydrolysis reaction of l-menthyl acetate to produce l-menthol. The best result is observed at 16 hours with ethanol solvent, which give % conversion about 13,76%. The research followed by in silico using the docking method, we observed the mechanism between amino acid residues to macromolecules as well as the influence of solvents on such interactions. The residues that involved in the reaction are Glu, Thr, Asp, Gln, Ser, His. The ethanol solvent shows a good interaction with macromolecules by forming hydrogen bonds.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/396/051709736 |
| Uncontrolled Keywords: | l-mentil asetat, l-mentol, enzim lipase dari Candida antarctica recombined Aspergillus oryzae, docking |
| Subjects: | 500 Natural sciences and mathematics > 541 Physical chemistry > 541.3 Miscellaneous topics in physical chemistry > 541.39 Hydrolysis |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Oct 2017 08:10 |
| Last Modified: | 22 Nov 2021 04:10 |
| URI: | http://repository.ub.ac.id/id/eprint/4058 |
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